Will depend on whether pair (1 female to 1 male), trio (2 female to 1 male) or harem mating is used.
N equals number of pseudopregnant females to receive manipulated embryos. Estimate as follows:
number of viable embryos = number of blastocysts injected × expected % of blastocysts viable after injection
N equals estimated ratio of nontransgenic to transgenic animals to be weaned from original number of blastocysts. Estimate as follows:
N equals number of animals to be mated to transgenic animals. Estimate as follows:
N = desired number of transgenic founder × number of animals to be mated to each transgenic animal to reach breeding age
The desired number of potential founders may be predetermined by the investigator. A common number is 6-10 potential founders for a single cDNA construct.
N equals number of offspring to be genotyped. Estimate as follows:
N = number of offspring per transgenic animal × expected number of transgenic animals actually bred
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