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Dietary Supplements: A Framework for Evaluating Safety (2005)

Chapter: 6 Categories of Scientific Evidence--Information About Related Substances

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Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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6
Categories of Scientific Evidence—Information About Related Substances

It is scientifically acceptable and appropriate to use information about safety concerns of related substances to inform a decision about the risk associated with a dietary supplement ingredient, especially in the absence of information about the activity of the ingredient in question in humans, animals, or in vitro experiments.1 Information about substances related to the dietary supplement ingredient of interest may be helpful when predicting risk in one of the following ways:

  • Chemical relatedness—similarity to known toxic chemicals or presence of constituents similar in structure to known toxicophores. Chemical structures associated with potential adverse effects;

  • Taxonomic relatedness—similarity to known toxic plant species, genus, or family; and

  • Functional relatedness—the dietary supplement ingredient of interest is related to another substance because they share a common biological target or mechanism of action that is clearly tied to a toxic effect. This includes endogenous substances and mimetics of endogenous substances when the effect of increasing the amount of an endogenous substance is linked to an adverse health effect.

1  

Note that this chapter describes the application of information on risk and safety concerns of related substances to the dietary supplement ingredient in question, not the converse (using information suggesting safety to mitigate concerns about the dietary supplement ingredient).

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

The value and utility of these types of information, taken together, to predict risk depends on the type of dietary supplement ingredient that is being considered. Cause for concern with a botanical dietary supplement may be derived from information about risk associated with known chemical constituents, as well as information about risk associated with related toxic plants. Similarly, information about the potential risk of dietary supplements that are pure single chemical compounds may be derived by reviewing a list of known risk-associated chemical compounds and chemical moieties (toxicophores) that raise concern of safety. However, for information about what might occur following ingestion of substances that are normally present in the human body (endogenous substances), it is helpful to understand what the substances do in the body at normal concentrations and to understand their mechanisms of action well enough to shed light on what might occur if the normal concentrations are exceeded. Certainly, for particular dietary supplement ingredients, such information could be more useful than reviewing a list of unrelated toxic chemical structures or substances that are not endogenous. Finally, especially when dietary supplements have undefined chemical composition2 but information about biological activity is available, it may be helpful and it is appropriate to consider whether the exhibited biological activity is the basis for safety concerns of other substances that are considered potentially harmful. Provided below are guiding principles and further descriptions of the different types of “relatedness” information, including discussion of when and why it is appropriate to use this type of information and specific questions that may help in extrapolating the most useful information.

CONSIDERING INFORMATION ABOUT CHEMICAL COMPONENTS AND RELATED BOTANICALS AS SIGNALS OF POTENTIAL RISK OF BOTANICAL DIETARY SUPPLEMENT INGREDIENTS

GUIDING PRINCIPLE: Consumption of any botanical ingredient carries a certain degree of inherent risk to at least some segments of the human population, even for those plants used as foods or with a history of use for medicinal purposes. In the absence of comprehensive human trials establishing safety, scientific evidence for risk can be obtained by considering whether the plant constituents are

2  

An example of a nonbotanical dietary supplement with undefined chemical composition might be a preparation from a living organism or otherwise complex substance—shark cartilage is an example.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

compounds with established toxicity or closely related in structure to compounds with established toxicity, or whether the plant source of the botanical dietary supplement is itself a toxic plant or is taxonomically related to a known toxic plant.

It is well known that plants produce secondary metabolites with biological activities in mammals, and that plant toxicities are due to chemical constituents in plants. Indeed, the rationale for the use of botanical dietary supplements is that they are likely to affect human function. The challenge in assessing risk in the use of dietary supplements is to establish whether the plant compounds present a hazard to humans and, if so, whether the conditions of use suggest risk.

Risk is always considered a function of two factors: hazard and exposure. In the case of botanical ingredients, hazard relates to the presence of biologically active metabolites produced naturally by biosynthetic processes within the plant. In contrast, exposure may be a consequence of the amount of any particular substance produced by the plant, its concentration or dilution during manufacture, and user intake level and bioavailability (see Chapter 3). Thus consumption of a botanical containing a high level of potentially dangerous bioactive substances, consumed at high dosages or for prolonged periods, will significantly increase risk.

It is possible to make educated estimates of the potential hazard of any given botanical through consideration of the types of biologically active compounds that may be present in the plant (constituents of concern) and the nature of the plant (taxonomic relationships). The goal is to consider two likely scenarios that could provide some guidance regarding the possible toxicity of a botanical dietary supplement ingredient (1) where a known constituent of the plant is, or is structurally similar to, a known toxic compound; and (2) where a plant genus or species is, or is closely related to, a plant known to be toxic. When there is evidence that a botanical is taxonomically related to known poisonous plants and that particular constituents are established as having deleterious effects, the convergence of these factors compels detailed consideration of the potential risks associated with the use of the ingredient.

Information about the potential biological activity of a plant-derived dietary supplement ingredient is obtained by reviewing information about the plant’s individual chemical components to determine if any of the constituents raise concerns. Given that related plants have related chemical composition, with more closely related plants generally having more similar chemical constituents, it is therefore also appropriate to consider the activity of other plants in the same plant family or genus to predict composition and potential toxicity.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

This approach of considering the taxonomic relatives of the dietary supplement ingredient has its limitations, however. That is, not all genera of a given family will contain similar toxic components. Furthermore, the concentration of potentially dangerous compounds in the final product will be affected by the plant part being utilized and the manner of preparation, processing, and formulation, as well as by growth conditions that can produce variation in chemical constituents (e.g., climate, season, soil conditions).

Chemical Constituents of Concern

Secondary metabolites of plants are generally low-molecular-weight compounds (~ < 1000 Daltons), originally thought to be biosynthesized by the plant primarily for purposes other than basic nutritional and metabolic requirements for normal growth and reproduction (Harborne, 1993). When produced, these metabolites confer “fitness” on the plant, enabling it to respond to and counteract external influences, such as competition for resources, environmental stresses, herbivory, and microbiological or viral attack. The biosynthetic mechanisms by which certain of these compounds (phytoalexins) are produced may essentially shut down unless there is an external stimulus that triggers their production for defensive purposes (Fong, 2002; Harborne, 1993). Other compounds may always be present because evolutionary pressures have established their necessity.

Known Constituents of Concern

Known chemicals and classes of chemicals that are botanical constituents and warrant concern for safety are listed in Box 6-1, a list generated largely from consideration of plant genera of concern identified in the next section of this chapter. Some of the mechanisms of these compounds, as well as information about plants containing them, are described in the discussion of plant families in Appendix C. (Other compounds or classes of compounds act through mechanisms that are only theoretical or are not understood.) Appendix C describes how some of these compounds are ingested in conventional foods where the amounts ingested are limited or are in different forms due to processing (e.g., cooking). This list is not intended to be all inclusive, but rather to highlight some of the compounds that may result in adverse effects from ingesting plants. Some of these compounds cause more serious deleterious effects than others and some compounds are more potent than others. It is suggested that this list be taken as a general guideline helpful to the Food and Drug Administration (FDA) in determining which botanical substances may warrant higher priority attention. Evidence that one or more of these chemical constituents is

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

present in a botanical dietary supplement should be considered as an indicator of increased concern for potential toxicity of the specific botanical product, except when consumed as constituents of conventional foods, unless additional information mitigates concern. Further investigation may result in mitigated concern if it is found that circulating concentrations of constituents resulting in adverse effects are substantially lower than circulating concentrations reached with dietary supplement ingestion or if quality animal toxicity studies suggest that the effects are unlikely to occur from the amounts or preparations ingested as dietary supplements. As indicated, some of the substances listed are classes of compounds rather than individual chemical constituents. In this case, some members of a given class may be of less or no concern (see also Appendix C), as will be uncovered by a search of the available literature. For example, a literature search may reveal conclusive evidence that specific structural features required for toxicity are not present for some members of a given class.

Of all classes of botanical toxic compounds, those classified as alkaloids predominate in causing concern because a large proportion have been associated with biological activities and/or toxic effects in mammals (Harborne, 1993; Seawright et al., 1985). Thus particular attention is warranted for dietary supplement ingredients containing alkaloids. Although most chemists recognize and agree on whether a particular compound is an alkaloid, there has been considerable discussion as to how to define such compounds simply because they do not conform to a single structural type. The most workable definition is probably that of Pelletier (1983), which states that “an alkaloid is a cyclic organic compound containing nitrogen in a negative oxidation state which is of limited distribution among living organisms.”3 This definition excludes simple amines, amino acids, peptides, proteins, nucleic acids, and nucleotides, which are ubiquitous, as well as nitro compounds such as aristolochic acid, in which the nitrogen is not in a negative oxidation state. It is particularly noteworthy that the definition does not carry a requirement for pharmacological activity. This is appropriate because many newly isolated alkaloids may not have been tested, and even those of long standing will not have been evaluated for each and every type of activity. Nevertheless, alkaloid-containing plants should always be suspected of being capable of pharmacological activity and should be considered as risk factors.

3  

Many other definitions often include a statement that alkaloids usually are biologically active (Cordell et al., 2001).

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

BOX 6-1
Specific Botanical Chemical Constituents of Concern and General Classes of Constituents of Concern

Constituents

Classes of Constituents

3-hydroxy-4(1H)-pyridone

4’-demethylpodophyllotoxin

abrina

aconitine and pseudoaconitine

anemonin

amygdalin

arecoline

atractyloside

australine

beta-phenethylamine

canavanine

carboxyatractyloside

castanospermine

colchicine1

convallatoxin

coriamyrtin

cycasin

cyclopamine

cytosine

dicoumarol

digitoxin

digoxin

fagopyrine

galanthamine

helenalin, hymenovin

hydrogen cyanide

hyoscyamine

hypoglycin A

indospicine

isocupressic acid

isoperoxisomicine A-1

lantadene A and B

lycoctonine

lyoniatoxin

macrozamin

mandelonitrile

methyllycaconitine

mimosine

monocrotaline

nicotine

β-nitropropionic acid

nordihydroguaiaretic acid

norephedrine (phenylpropanolamine)

α-peltatin

peroxisomicine A-1

agroclavine alkaloidsa,b

alkenyl catechols (urushiols)

amino-butyric and -propionic

acids and nitriles

anthracene dimers

aristolochic acidsa

bufadienolidesa

cardenolides,a

cardiac glycosides

coumarins

cyanogenic glycosides (phaseolunatin) and other cyanogenic compoundsa

cyclopropane amino acids

dibenzofurans

diterpene acids

diterpenoid alkaloidsa

ephedrine and related alkaloids

ergot alkaloidsa,b

furanoeremophilanes

glucosinolatesc

grayanotoxins

indole alkaloidsa

indolizidine alkaloidsa

isoquinoline alkaloidsa

labdane diterpene acids

methylazoxymethanol glycosides

morphinan alkaloids

nicotine alkaloidsa

nitrates, nitrites

nitrophenathrenesa

nitrosaminesa

nojirimycin and derivatives

oxalates

penitrem alkaloidsa,b

phenalenones

phenylpropanolamine

phorbol estersa

piperidine alkaloidsa

podophyllotoxin-type lignans

polyacetylenes

polyhydroxy alkaloids (swainsonine, calystegines)a

polyhydroxy nortropanes

pressor amines

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Constituents

Classes of Constituents

phenylpropanolamine

physostigmine (eserine)

picrotoxin

podophyllotoxin

protoanemonin

N-propyl disulfide

prunasin

ptaquiloside

ranunculin

ricina

safrole

sanguinarine

scopolamine

senecionine

seneciphylline

solanine

solasonine

sparteine

strychnine

tetradymol

tomatine

trichodesmine

tutin

tyramine

zearalenone

zygacine

zygadenine

pyrrolizidine alkaloidsa

quinolizidine alkaloidsa

rotenoids

steroidal alkaloidsa

taxoids

thiooxazolidones

tremetone and derivatives

trichothecenes

tropane alkaloidsa

NOTE: The chemical constituents and general classes of constituents listed in this table were largely derived from the list of botanicals in Table 6-1 by committee members knowledgeable in botanicals and phytochemicals and who consulted the references listed at the end of the chapter as needed. A review of each chemical on this list was not practical within the constraints of this report. The list should thus be considered as a general guideline for determining which chemical constituents and classes of constituents warrant attention, not as an authoritative statement on any chemical constituent or class in particular. When consumed as minor constituents of conventional foods prepared via conventional methods, these constituents are of little concern.

a  

Particularly hazardous, would probably be considered as “A” toxicity as defined in toxicity descriptions in Table 6-1 (A: Reports of adverse effects to the heart, liver, lungs, kidney, immune system, and reproductive system, teratogenicity, carcinogenesis, central nervous system [convulsant], or death in animals or humans).

b  

Depending on dose, alkaloids could be considered as exhibiting A, B, or C level of potential toxicities.

c  

And isothiocyanates, the toxic hydrolytic products of the naturally occurring glucosinolates (glycosides).

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×
Identifying Other Chemical Constituents in Botanicals That May Be of Concern

In addition to known chemicals highlighted in Box 6-1, other structurally related chemical constituents should also be of concern, unless there is convincing information suggesting that particular structural features are required for toxicity and these are not present. It is important to note that if a botanical is known to contain a chemical constituent that is structurally related to a chemical that is regulated (e.g., as a drug), this is a reason for concern and should be investigated. It is not possible to specifically define all the ways that different chemicals may be related, but this concept can be illustrated with an example. Substances with similar chemical structures, such as ephedrine and amphetamine, are structurally related, and substances that stimulate or inhibit activity at the same cellular receptors or other biological targets are “functionally” related (see discussion later in this chapter). Similarity of dietary supplement ingredients to biologically active metabolic intermediates, such as cytokines or hormones, may also be important if the actions of metabolic intermediates provide clues about the activity of a dietary supplement ingredient; this concept is discussed in the “endogenous substances” section.

Taxonomic Relationships and Genera of Concern

GUIDING PRINCIPLE: If a botanical dietary supplement was derived from a plant that belongs to a genus known to contain toxic compounds, it is scientifically reasonable to presume that the same compounds will be present in the dietary supplement and thus may pose a risk from ingestion of the ingredient, unless there is reason to believe that the plant or plant part being used does not cause the effect or contain the toxic substances.

Frequently, information about the chemical constituents or the distribution of chemical constituents throughout a plant used to make a dietary supplement will not be complete. In this case, it will be helpful and appropriate to consider whether a botanical is related to plants that are of concern.4 The system of naming, ranking, and classifying plants and other

4  

It is also appropriate to consider information about related plants even when information about the chemical constituents of the botanical in question is available.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

organisms based on morphology is now being guided by analysis of metabolites and molecular genetics. There is no doubt that, as progression is made to an increasing degree of specialization through the hierarchy sequence of family, genus, species, subspecies/variety/cultivar, and plant organ, there will be a corresponding increase in congruence, not only in physical appearance, but also in the nature of secondary metabolites produced and sequestered by the plant. Therefore, as any group of plant species becomes more closely related, the compounds biosynthesized will become more similar in both structural types and specific constituents. Thus, in summary, evidence that a botanical bears a close taxonomic relationship to known toxic plants should be used to evaluate potential human risk in the absence of scientific information that such data are not relevant. (See Box 6-2 for a summary of questions to be asked and Box 6-3 for notes on botanical nomenclature.)

Plants in the same genera will not necessarily produce compounds with exactly the same structure, but they are likely to produce the same structural classes of compounds. For example, different species of the genus Senecio (Asteraceae), in spite of being widely distributed in many parts of the world and growing under vastly different climatic conditions, are invariably found to contain pyrrolizidine alkaloids on phytochemical examination (Hartmann and Witte, 1995). Since chemical structure and biological activity are intimately related, novel pyrrolizidine alkaloids should be assumed to possess at least some degree of the hepatotoxic activity established for the most common members of this class (Hartmann and Witte, 1995) if information to prove otherwise is not available. (In this case, data suggest that hepatotoxicity of pyrrolizidine alkaloids depends on unsaturated 1,2 bonds in one of the rings [Hartmann and Witte, 1995].) For the purposes of this framework, taxonomic classification helps in identifying plants that are likely to have similar chemical components. Therefore, much information can be gained by reviewing what is known about plants that are taxonomically related to the dietary supplement ingredient under consideration.

The chemical composition of a given plant species can vary depending on the conditions under which it was grown. However, it is rare for a chemical compound to be observed in one specimen of a species, but not in another specimen of the same species, except due to artifactual differences in analysis techniques. It is more likely that differences in the levels of particular compounds will be observed (Fong, 2002; Harborne, 1993). This is because the array of phytochemicals that a given species may contain is under genetic control; thus each plant has the potential to create the same range of phytochemicals. While the environment and growth conditions may impact phytochemicals found in a given plant, plants of a species known to contain harmful phytochemicals under some conditions should

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

BOX 6-2
Questions to Be Asked Regarding Taxonomic Relationships of Botanicals

  • Is the supplement formulated from a plant that is in a known toxic plant family?

  • Is the supplement classified in a genus known to contain toxins or suspected of being unsafe?

  • Is the supplement classified in a species/subspecies/variety/cultivar known to contain toxins?

  • Does the supplement contain a particular plant part known to contain toxins in a dangerous level?

BOX 6-3
Notes About Botanical Nomenclature

When searching for chemical or pharmacological information, it should be kept in mind that plant synonymy is often encountered at both the family and genus level. Although several nomenclature systems have been used over the years and changes by taxonomists are encountered, the dual names are found primarily in a few plant families, namely:

Compositae

=

Asteraceae

Cruciferae

=

Brassicaceae

Graminae

=

Poaceae

Guttiferae

=

Clusiaceae

Labiatae

=

Lamiaceae

Leguminosae

=

Fabaceae

Palmae

=

Arecaceae

Umbelliferae

=

Apiaceae

Nomenclature changes were made in those plant families ending in -ae so that all plant families would have the conventional -aceae ending.

At the genus level nomenclature is constantly being changed or corrected. For example, one can find chemical and pharmacological information in the literature for the plant Catharanthus roseus under the binomials Vinca rosea, Lochnera rosea, and Ammocallis roseus, which are all the same plant. At the beginning of a literature search it is essential to know the specific parameters to be used with regard to botanical terminology. It is suggested that the Index Kewensis (online) can be used, but botanical expertise is necessary to interpret the data (IPNI, 2004). A less complicated reference source to ascertain correct Latin binomials and family classification for a given plant or group of plants is Mabberley (1997). One can obtain the following information from this reference:

Search

Find

a. Common Name

Latin Binomial

b. Genus Name

Possible synonymy and common uses, including (some) toxicity

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

be assumed to have them even if grown under different conditions. Analyses that suggest specimens of a given plant species do not contain a hazardous phytochemical usually associated with the plant should be carefully considered to ensure that the analysis techniques are appropriately sensitive.

The presence of toxic compounds has been traditionally associated with a number of plant genera and families (e.g., Liliaceae are known to contain cardiac glycosides, Euphorbiaceae are known to contain phorbol esters and toxic diterpenes). The ability to anticipate the presence of specific classes of compounds based on plant family and genus knowledge may be helpful in predicting potential toxicity. Table 6-15 highlights some of the plant genera to which FDA may want to give attention. Also important are the nuances of information about each plant family, which are discussed in Appendix C. It is important to note that this table is not intended to provide a complete reference or to be inclusive, but it serves to provide FDA with a starting point of plant genera that warrant concern. The primary difficulty in using information about related plants to infer information about the toxicity of a particular plant arises when the family encompasses both valuable food plants and species capable of producing toxic compounds (see discussion of traditional use as a food plant below and specific examples in Appendix C).

Considerations Regarding Concerns Raised by Taxonomic Relationships or Chemical Constituents of Concern

There are a number of considerations that may mitigate or exacerbate concerns raised by the taxonomic relationship of a dietary supplement ingredient to a hazardous botanical or knowledge that a botanical contains chemical constituents of concern. These are described here, followed by discussion of how these and other factors should impact the use of historical consumption information as a mitigator of concern.

Plant Parts

Chemical compounds are differentially distributed in various parts of plants. When secondary metabolites are biosynthesized for the purpose of

5  

Note that association of these plants with toxic effects may well be a reflection of the degree of phytochemical examination to which they have been subjected, given that there is more reason to investigate plants that are cultivated for specific purposes. Other plant families may be relatively neglected (phytochemically), especially those that are of limited distribution or occur in remote areas.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

TABLE 6-1 Genera of Concern

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Abrus

Fabaceae

Abrin

Aconitum

Ranunculaceae

Diterpenoid alkaloids

Actaea

Ranunculaceae

Quinolizidine alkaloids

Adenostyles

Asteraceae

Pyrrolizidine alkaloids

Agave

Agavaceae

Saponins

Agrostemma

Caryopyhllaceae

Saponins

Aleurites

Euphorbiaceae

Unknown

Alliumb

Liliaceae

N-propyl disulfide

Amsinckia

Boraginaceae

Pyrrolizidine alkaloids

Anamirta

Menispermaceae

Picrotoxin

Anchusa

Boraginaceae

Pyrrolizidine alkaloids

Anemone

Ranunculaceae

Protoanemonin

Apiumb

Apiaceae

Coumarins

Apocynum

Apocynaceae

Cardiac glycosides

Areca

Arecaceae

Arecoline

Argemone

Papaveraceae

Isoquinoline alkaloids

Arisaema

Araceae

Oxalates

Aristolochia

Aristolochiaceae

Aristolochic acids

Armoracia

Brassicaceae

Isothiocyanates, Thiooxazolidones

Arnica

Asteraceae

Sesquiterpenes

Asclepias

Asclepidaceae

Cardiac glycosides

Astragalus

Fabaceae

Polyhydroxy alkaloids (swainsonine)

Nitro-alcohols and -acids,

Selenium accumulation

Atractylis

Asteraceae

Atractyloside and related compounds

Atropa

Solanaceae

Tropane alkaloids

Avenab

Poaceae

Nitrate accumulation

Baccharis

Asteraceae

Trichothecenesc

Baileya

Asteraceae

Oxalates and nitrates

Baptisia

Fabaceae

Quinolizidine alkaloids

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Hemorrhagic

A

Only seeds are toxic

Muscle and respiratory failure, death

A

 

Gastrointestinal, circulatory

B

Fruits and roots are toxic

A. racemosa syn. Cimcifuga racemosa (black cohosh)

Hepatotoxic, carcinogenicity

A

 

Photosensitization, hepatotoxic

B

 

Gastroenteritis, coma, death

A

 

Hemorrhagic

B

 

Gastritis, vomiting, diarrhea

C

 

Hepatotoxic, carcinogenic

A

 

Convulsant

A

 

Hepatotoxic, carcinogenic

A

 

Gastroenteritis, death

B

 

Coagulapathy, photosensitization

C

 

Cardiotoxic, death

A

 

Carcinogenic

A

Usually mixed with other plants

Dropsy, glaucoma, death

A

 

Numbness of mouth and throat

B

Common household plant

Nephrotoxicity, carcinogenic

A

All species

Goitrogenic, death

B

Only in exceptionally large amounts

Gastrointestinal, coma

C

 

Cardiotoxic, coma

A

 

Abortifacient, neurotoxic; teratogenic

Respiratory failure, death

Anorexia, emaciation, neurotoxicity, death

A

Variable toxicity depending on species

Hepatotoxic, nephrotoxic

A

 

Anticholinergic

A

 

Anoxia

A

 

Gastrointestinal

B

Variable toxicity based on species

Hepatotoxic, nephrotoxic

C

Large amounts in livestock

Teratogenic, respiratory failure, death

A

 

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Betab

Polygonaceae

Oxalates

Nitrates

Blighia

Sapindaceae

Cyclopropane amino acids

Borago

Boraginaceae

Pyrrolizidine alkaloids

Brassicab

Brassicaceae

Isothiocyanates

Glucosinolates

Thiooxazolidones

Brugmansia

Solanaceae

Tropane alkaloids

Buxus

Buxaceae

Steroidal alkaloids

Cacalia

Asteraceae

Pyrrolizidine alkaloids, Triterpene

Calystegia

Convolvulaceae

Polyhydroxy nortropane and indolizidine alkaloids

Canavalia

Fabaceae

Canavinine

Hydrogen cyanide

Indospicine

β-nitropropionic acid

Nitrites

Castanospermum

Fabaceae

Polyhydroxy alkaloids (castanospermine)

Castilleja

Scrophulariaceae

Pyrrolizidine alkaloids

Centaurea

Asteraceae

Sesquiterpene lactones

Chelidonium

Papaveraceae

Isoquinoline alkaloids

Cicuta

Apiaceae

Polyacetylenes

Colchicum

Liliaceae

Colchicine

Colocasia

Araceae

Oxalates

Conium

Apiaceae

Piperidine alkaloids

Convallaria

Liliaceae

Cardiac glycosides

Convolvulus

Convolvulaceae

Polyhydroxy nortropane and indolizidine alkaloids (calystegines and swainsonine)

Coriaria

Coriariaceae

Picrotoxin-like terpenes

Corydalis

Papaveraceae

Isoquinoline alkaloids, bicuculline

Crotalaria

Fabaceae

Pyrrolizidine alkaloids

Croton

Euphorbiaceae

Phorbol esters

Cupressus

Cupressaceae

Labdane diterpene acids

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Gastrointestinal, depression, death

C

Only seeds are toxic

Hypoglycemia, convulsions, death

A

Unripe fruits are toxic

Hepatotoxic, carcinogenic

A

 

Irritant to mucus membranes, death

C

Only toxic at very high levels

Anticholinergic

 

Atropine related compounds

Convulsant

A

All plant parts

Hepatotoxic, carcinogenic

A

 

Neurotoxicity

A

 

Nephrotoxic, respiratory failure

A

Only in very large amounts

Neurotoxic, gastroenteritis

A

 

Hepatotoxic, carcinogenic

A

Toxins acquired from other plants through parasitism

Neurotoxicity

A

 

Dropsy, glaucoma, death

A

 

Convulsant, respiratory failure

A

 

Antimitotic

A

 

Numbness of mouth and throat

B

 

Teratogenic, respiratory failure, death

A

 

Cardiotoxic

A

 

Neurotoxic

B

Some species are not toxic

Analeptic

A

 

Convulsant

A

 

Carcinogenic, pulmonary, hypertension

A

 

Gastroenteritis, cocarcinogen

A

 

Abortifacient

A

Mainly in livestock

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Cycas

Cycadaceae

Methylazoxymethanol glycosides

Cynodon

Poaceae

Cyanogenic glycosides

Agroclavine alkaloidsc

Cynoglossum

Boraginaceae

Pyrrolizidine alkaloids

Cytisus

Fabaceae

Quinolizidine alkaloids

Daphne

Thymelaeaceae

Phorbol esters

Datura

Solanaceae

Tropane alkaloids

Daucusb

Apiaceae

Polyacetylenes

Delphinium

Ranunculaceae

Diterpenoid alkaloids

Descurainia

Brassicaceae

Isothiocyanates

Thiooxazolidones

Dicentra

Papaveraeae

Isoquinoline alkaloids, Bicuculline

Dieffenbachia

Araceae

Oxalates

Digitalis

Scrophulariaceae

Cardiac glycosides

Dolichos

Fabaceae

Cyanogenic compounds

Drymaria

Caryophllaceae

Saponins

Duboisia

Solanaceae

Tropane alkaloids

Echium

Boraginaceae

Pyrrolizidine alkaloids

Ephedra

Ephedraceae

Ephedrine, Norephedrine and related alkaloids

Euonymus

Celastraceae

Cardiac glycosides

Eupatorium

Asteraceae

Pyrrolizidine alkaloids

Tremetol/tremetones

Euphorbia

Euphorbiaceae

Phorbol esters

Fagopyrum

Polygonaceae

Fagopyrine

Farfugium

Asteraceae

Pyrrolizidine alkaloids

Ferula

Apiaceae

Coumarins

Festuca

Poaceae

Ergot alkaloidsc

 

 

Penitrem alkaloidsc

Selenium accumulation

Flourensia

Asteraceae

Tremetone derivatives,

Benzofurans, Polyacetylenes

Gloriosa

Liliaceae

Colchicine

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Carcinogenic, neurotoxic, hepatotoxic

A

 

Respiratory failure

Neurotoxic

Ad

 

Hepatotoxic, carcinogenic

A

 

Respiratory failure, coma, death teratogenic

A

 

Cocarcinogen, allergenic

A

 

Anticholinergic

A

Atropine-like compounds

Gastroenteritis

C

 

Muscle and respiratory failure, cardiotoxic, death

A

 

Goitrogenic

B

 

Convulsant

A

All parts are toxic

Numbness of mouth and throat

B

Common household plant

Cardiotoxic

A

 

Cardiotoxic respiratory failure, death

A

Mainly seeds are toxic

Gastroenteritis, coma, death

A

 

Anticholinergic

A

Atropine-like compounds

Hepatotoxic, carcinogenic

A

 

Hypertension, death

B

Many species do not contain alkaloids. Toxicity is dependant on amount

Cardiotoxic, coma, death

A

Fruits and leaves are toxic

Hepatotoxic, carcinogenic

Gastroenteritis

A

 

Vesicant, cocarcinogenic

A

 

Photosensitization, allergenic

C

 

Hepatotoxic, carcinogenic

A

 

Photosensitization, coagulapathy

C

 

Vasoconstriction, gangrene, tremors (See Lolium)

A/Bd

Caused by fungal contamination

Anorexia, emaciation, neurotoxicity, death

 

 

Death

B

 

Antimitotic

A

 

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Gutierrezia

Asteraceae

Diterpene acids (?)

Halogeton

Polygonaceae

Oxalates

Nitrates

Haplopappus

Asteraceae

Tremetol/tremetone derivatives

Hedera

Araliaceae

Saponins

Helenium

Asteraceae

Sesquiterpene lactones (helenalin, hymenovin)

Heliotropium

Boraginaceae

Pyrrolizidine alkaloids

Helleborus

Ranunculaceae

Cardiac glycosides

Hippomane

Euphorbiaceae

Phorbol esters

Hyacinthus

Liliaceae

Unknown

Hymenoxys

Asteraceae

Sesquiterpene lactones (helenalin, hymenovin)

Hyoscyamus

Solanaceae

Tropane alkaloids

Illicium

Illiciaceae

Sesquiterpenes

Indigofera

Fabaceae

Canavanine

Hydrogen cyanide

Indospicine

β-nitropropionic acid

Nitrite

Ipomoeab

Convolvulaceae

Polyhydroxy nortropane and indolizidine alkaloids

Jatropha

Euphorbiaceae

Diterpenes

Juniperus

Cupressaceae

Labdane diterpene acids

Kalmia

Ericaceae

Grayanotoxins

Karwinskia

Rhamnaceae

Quinones, peroxisomicine A-1 (anthracene dimers)

Laburnum

Fabaceae

Quinolizidine alkaloids

Lantana

Verbenaceae

Triterpenes (lantadene A and B)

Larrea

Zygophyllaceae

Nordihydroguaiaretic acid

Lathyrus

Fabaceae

Aminobutyric and aminopropionic acids and nitriles

Leucaena

Fabaceae

Mimosine

Liatris

Asteraceae

Pyrrolizidine alkaloids

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Abortifacient

A

Mainly in livestock

Indigestion

Depression, death

A

Large amounts in livestock

Hepatotoxic, gastrointestinal

A

 

Respiratory failure, coma, death

B

Only one species implicated of many

Gastrointestinal, hepatotoxic, nephrotoxic, death

A

 

Hepatotoxic, carcinogenic

A

 

Convulsant, cardiotoxic

A

 

Cocarcinogen

A

 

Gastrointestinal

B

Mainly bulbs are implicated

Gastrointestinal, hepatotoxic, nephrotoxic

A

 

Anticholinergic

A

Atropine derivatives

Convulsant

A

All parts of plants

Abortifacient, gastroenteritis, nephrotoxic, hepatotoxic

A

See Canavalia

Neurotoxicity

B

Many species are not toxic

Neurotoxicity

B

Mainly seeds

Abortifacient

A

Mainly in livestock

Cardiotoxic

A

 

Neurotoxic, nephrotoxic

A

Mainly fruits are implicated

Respiratory failure, coma, death teratogenicity

A

 

Hepatotoxic, anithrombin, possible anticholinergic

A

 

Hepatotoxic, nephrotoxic

A

 

Neurotoxic, neurolathyrism (skeletal deformity)

A

 

Hair loss, cytotoxic, goitrogenic

A

Mimosine is metabolized to 3-hydroxy-4(1H)-pyridone

Hepatotoxic, carcinogenic

A

 

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Ligustrum

Oleaceae

Unknown

Lobelia

Campanulaceae

Piperidine alkaloids

Lolium

Poaceae

Ergot alkaloidsc

Penitrem alkaloidsc

Selenium accumulation (Selenium is acquired from Seleniferous soils, not funga)

Lupinus

Fabaceae

Quinolizidine alkaloids

Lycopersiconb

Solanaceae

Steroidal alkaloids

Polyhydroxy alkaloids (calystegines)

Lyonia

Ericaceae

Lyoniatoxin

Macrozamia

Zamiaceae

Methyl azoxymethanol glycosides (i.e., macrozamin)

Manihotb

Euphorbiaceae

Cyanogenic glycosides

Melilotusb

Fabaceae

Dicoumarol

Myristicab

Myristicaceae

Safrole

Narcissus

Amaryllidaceae

Isoquinoline alkaloids

Nerium

Apocynaceae

Cardiac glycosides

Nicotiana

Solanaceae

Nicotine alkaloids

Nolina

Liliaceae

Unknown

Ornithogalum

Liliaceae

Colchicine

Oxytropis

Fabaceae

Polyhydroxy alkaloids (swainsonine)

Pachyrhizusb

Fabaceae

Unknown

Packera

Asteraceae

Pyrrolizidine alkaloids

Papaver

Papaveraceae

Isoquinoline alkaloids

Morphinan alkaloids

Pedicularis

Scrophulariaceae

Pyrrolizidine alkaloids

Petasites

Asteraceae

Pyrrolizidine alkaloids

Phaseolusb

Fabaceae

Cyanogenic glycoside (phaseolunatin)

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Gastroenteritis, death

A

Mainly fruits and leaves

Respiratory failure, coma, death

B

Mainly large doses in livestock

Vasoconstriction, gangrene Tremors

A/Bd

Caused by fungal contamination

Anorexia, emaciation, neurotoxicity, death

 

 

Respiratory failure, coma, death teratogenic

B

Many species (“sweet lupines”) are not toxic

Gastroenteritis, teratogenic

Gastroenteritis, neurotoxicity (i.e., same as Solanum)

B

Restricted occurrence of toxic substances

Convulsant

A

All parts of the plant

Hepatotoxic, neurotoxic, carcinogenic

A

 

Respiratory failure, death

A

Cyanogenic toxins are removed by boiling or heating when prepared as food

Hemorrhagic

B

Dicoumarol is formed by microbial transformation (fermentation)

Carcinogenic

A

 

Cardiotoxic, external irritant, emetic death

A

 

Cardiotoxic, gastrointestinal, death

A

 

Cholinergic toxicity

A

 

Hepatoxic, gastroenteritis

A

 

Animitotic

A

 

Neurotoxic, teratogenic, abortifacient, gastroenteritis

A

 

Convulsant

A

Mainly seeds, root edible (jicama)

Hepatotoxic, carcinogenic

A

 

CNS effects

A

 

Hepatotoxic, carcinogenic

A

 

Hepatotoxic, carcinogenic

A

 

Respiratory failure, coma, death

B

Mainly in large amounts in livestock

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Phoradendron

Loranthaceae

Pressor amines

Physostigma

Fabaceae

Physostigmine (eserine)

Phytolacca

Phytolaccaceae

Saponins

Pilocarpus

Rutaceae

Pilocarpine

Pinus

Pinaceae

Labdane diterpene acids

Podophyllum

Berberidaceae

Podophyllotoxin-type lignans

Polygonatum

Liliaceae

Steroidal saponins

Prosopsis

Fabaceae

Indouizidine alkaloids,

β-nitropropionic acid, tyramine

Prunusb

Rosaceae

Cyanogenic glycosides

Psilostrophe

Asteraceae

Unknown

Pteridium

Polypodiaceae

Ptaquiloside

Ranunculus

Ranunculaceae

Protoanemonin

Rheumb

Polygonaceae

Oxalates, nitrates, anthraquinones

Rhododendron

Ericaceae

Grayanotoxins

Rhodomyrtus

Myrtaceae

Dibenzofurans

Ricinus

Euphorbiaceae

Ricin

Robinia

Fabaceae

Abrin

Rudbeckia

Asteraceae

Unknown

Rumex

Polygonaceae

Oxalates, nitrates

Sambucus

Caprifoliaceae

Cyanogenic

Sanguinaria

Papaveraceae

Sanguinarine

Saponaria

Caryophyllyaceae

Unknown

Sarcobatus

Polygonaceae

Oxalates, nitrates

Sarothamnus

Fabaceae

Sparteine

Sassafras

Lauraceae

Safrole

Scilla

Liliaceae

Cardiac glycosides

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Gastritis, vasoconstriction, death

B

Large amounts in livestock

Cholinesterase inhibition, death

A

 

Extreme irritation on inhalation of root powder

B

 

Cholinomimetic

A

 

Abortifacient

B

Mainly in livestock

Gastroenteritis, catharsis, conjunctivitis

A

 

Cardiotoxic

A

 

Gastroenteritis, death

A

 

Cardiac and respiratory failure, death

A

 

Nephrotoxic

A

 

Carcinogenic

A

The fronds (fiddle heads) are processed (salting) and are safely eaten. All toxicities reported in livestock are from unprocessed fronds

Gastroenteritis, death

A

Mainly in large doses in livestock

Indigestion, depression, death

B

Mainly in livestock

Convulsant

A

All parts

Blindness

A

Mainly in livestock

Hemorrhagic, circulatory system

A

Only seeds are toxic

Hemorrhagic, circulatory system

A

 

Incoordination, gastroenteritis

C

Large amounts in livestock

Indigestion, Depression, death

C

Mainly in livestock

Cardiac and respiratory failure, death

A

 

Coma

A

Rhizomes

Gastroenteritis, coma, death

B

 

Indigestion, depression, death

B

Mainly in large amounts in livestock

Hypotension

B

Seeds and leaves

Carcinogenic

A

Bark, essential oils

Cardiotoxic, death

A

 

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Secaleb

Poaceae

Nitrate accumulation, ergot alkaloidsc

Senecio

Asteraceae

Pyrrolizidine alkaloids

Sesbania

Fabaceae

Quinolizidine alkaloids

Solanumb

Solanaceae

Steroidal alkaloids

Polyhydroxyalkaloids (calystegines)

Solidago

Asteraceae

Unknown

Sophora

Fabaceae

Quinolizidine alkaloids

Sorghumb

Poaceae

Cyanogenic glycosides, nitrate

Spartium

Fabaceae

Sparteine

Strelitzia

Musaceae

Phenalenones

Strychnos

Loganiaceae

Strychnine

Symphytum

Boraginaceae

Pyrrolizidine alkaloids

Tanacetum

Asteraceae

Unknown

Taxus

Taxaceae

Taxoids

Tetradymia

Asteraceae

Furanoeremophilanes

Teucrium

Lamiaceae

Diterpenes (?)

Thevetia

Apocynaceae

Cardiac glycosides

Toxicodendron

Anacardiaceae

Urushiols

Trichodesma

Asteraceae

Pyrrolizidine alkaloids

Trifoliumb

Fabaceae

Coumestrol

Isoflavones

Triglochin

Juncaginaceae

Cyanogenic glycosides

Tripterygium

Celastraceae

Diterpenes

Tussilago

Asteraceae

Pyrrolizidine alkaloids

Urginea

Liliaceae

Cardiac glycosides

Veratrum

Liliaceae

Steroidal alkaloids

Vicia

Fabaceae

Aminobutyric and aminopropionic acids and nitriles

Wisteria

Fabaceae

Canavanine

Xanthium

Asteraceae

Carboxyatractyloside

Zamia

Zamiaceae

 

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Anoxia, vasoconstriction, abortifacient, neurotoxic

A/Bd

Contamination with fungi

Hepatotoxic, carcinogenic

A

 

Hemorrhagic, circulatory system, collapse

A

 

Gastroenteritis, teratogenic

Gastroenteritis, neurotoxicity

A

 

Abortifacient, gastroenteritis

A

Large amounts in livestock

Respiratory failure, coma, death teratogenic

A

 

Respiratory failure

B

 

Abortifacient

A

 

Gastroenteritis, vertigo

B

Seeds and pods

Convulsant

A

 

Hepatotoxic, carcinogenic

A

All parts

Abortifacient, gastroenteritis, teratogenic

A

 

Convulsant and coma

A

 

Photosensitization

B

Large amounts in livestock

Nephrotoxic

A

Teucrium chamaedrys of major concern

Cardiotoxic, death

A

 

Dermatitis, gastroenteritis

A

Poison ivy, poison sumac, Poison oak

Hepatotoxic, carcinogenic

A

 

Photosensitization

Estrogenic

C

 

Respiratory depression

B

 

Immune suppressant, antispermatogenic

A

 

Hepatotoxic, carcinogenic

A

 

Cardiotoxic, death

A

 

Cyclopia, holoprosencephaly, limb defects

A

 

Neurotoxicity, teratogenic

A

 

Gastroenteritis

B

 

Heptotoxic, neurotoxic, death

A

 

Carcinogenic, neurotoxic, hepatoxic

A

 

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Primary Genera of Concern

Family

Compounds and Compound

Classes Implicated in Toxicity

Zeab

Poaceae

Zearalenone

Zephyranthes

Amaryllidaceae

Isoquinoline alkaloids

Zygadenus

Liliaceae

Steroidal alkaloids

NOTE: The information in this table was developed by committee members knowledgeable in botanicals and phytochemicals who consulted the references listed at the end of the chapter as needed. A review of each of the genera and families on this list was not practical within the constraints of this report. The list should thus be considered as a general guideline for determining which warrant attention, not as an authoritative statement on any in particular.

a A = reports of adverse effects to the heart, liver, lungs, kidney, immune system, reproductive system, teratogenicity, carcinogenesis, central nervous system (convulsant), or death in animals or humans, or where well-known constituents with adverse effects on these same organs, that is, pyrrolizidine alkaloids (liver), cardiac glycosides (heart), methylazoxymethanol glycosides (cancer) are reported for the genus. B = reports of nonpotentially lethal effects in humans or animals, such as severe irritation, gastric upset, emesis, photosensitization, or allergenicity. C = reports of effects that cannot be explained on the basis of known chemistry of the genus or where exceptional amounts, especially in livestock, were required to elicit the effect.

providing a protective function within the plant, they tend to be concentrated in young, tender leaves, shoots, and roots, or in reproductive structures (e.g., flowers and seeds). For example, livestock poisoning episodes have shown that there is often a bimodal distribution of toxic hazard with very young plants and plants at the reproductive stage being toxic, whereas at other growth stages no problems occur. (For a more complete discussion, see Appendix C and resources listed.) Frequently, compounds are continuously biosynthesized in a particular part of the plant, such as mature leaves where photosynthesis is at a maximum, but then they are transported and accumulated in other organs where the protective function conferred by such substances is required (Harborne, 1993).

Although it is possible for plants to contain completely different chemical entities in different parts, it is generally more likely that they will contain the same compounds or compounds that have undergone relatively minor structural transformations. The situation with respect to structural types of constituents is often under a state of continuous flux in response to environmental conditions and ecological factors. It is therefore appropriate to assume, in the absence of other information to the contrary, that a plant part marketed as a dietary supplement ingredient contains toxins that are found in other parts of the plant. That is, if a toxic chemical is present in one part of the plant, it will generally be present in the other parts of the plants, even if at lower concentrations. Indeed, concentrations of toxins

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

Observed or Predicted Toxicity

Classa

Other Comments

Vulvovaginitis in livestock

C

Caused by fungal contamination

Cholineesterase Inhibition

A

 

Gastritis, vasomotor collapse, coma, death

A

 

b Several species of these genera are common conventional food and/or condiment plants and are of little concern when consumed.

c Produced by endophytic fungi in some, but not all, cases.

d Class A concerns for Festuca stem from concerns about penitrem alkaloids and ergot alkaloids produced by fungal contamination, which can occur fairly frequently depending on weather conditions. Even if fungal contamination is not present, there are concerns when the plants grow in high-selenium soils, warranting at least a class B classification.

may vary and thus be less problematic in some plant parts, but the assumption should be that all parts of a plant containing toxins pose a risk unless there is credible evidence suggesting that dangerous levels of toxins are not present in the part marketed as a dietary supplement. In this case, selection of plant material at a specific growth stage to avoid incorporation of potentially toxic plant parts is desirable.

Cultivation and Other Conditions

In addition to concentration of toxic compounds in particular plant parts, levels of toxins in plants may also be influenced by growth stage, time of collection, environmental stress, herbivory, and a multitude of other factors (Fong, 2002). Blending of plant material from a number of locations will tend to dilute toxin levels that are higher in some plants if other plants are lower in toxin levels. However, in the absence of comprehensive studies, it is not possible to delineate precisely the overall influence of such conditions on constituent levels, although their role must be recognized in evaluating the safety of dietary supplements. When sporadic adverse incidents occur in association with a botanical supplement ingredient with no previous indication of risk, it may well be possible that environmental changes have resulted in an increase in levels of toxic constituents. If a plant’s content of a hazardous phytochemical varies significantly with environ-

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×

mental and growth conditions, then it is appropriate to consider the plant’s use in dietary supplements as a risk unless quality control or other actions are implemented to ensure that levels of compounds associated with risk are not reached in raw materials or finished products.

Preparation Methods

When considering the risk associated with a dietary supplement, it is important to consider whether the method of preparation is likely to concentrate toxic constituents or otherwise increase the consumption or bioavailability of toxic compounds. Toxic plant constituents that are normally present below a given threshold of toxicity can be concentrated by a variety of processing methods. Some methods of preparation may make specific toxins more readily available or even concentrate them (see also discussion of relevance of historical use in Chapter 4). An additional risk is that there may be a tendency to consume more of the plant material in an encapsulated form than if it were consumed in its “native” form.

Some methods of extract preparation can raise levels of toxic constituents to levels of greater concern. Whereas preparation of teas (i.e., aqueous infusions) is a method designed to concentrate specific constituents, many low-molecular-weight phytochemicals6 are not particularly water-soluble,7 and exceptionally high levels are not likely to be attained. Furthermore, hydrolytic changes may occur that can detoxify or reduce the levels of toxic compounds. It is also difficult to consume large volumes of teas. In contrast to aqueous extracts, extraction of plant material with alcohol or aqueous alcohol in which low-molecular-weight compounds are generally very soluble will likely concentrate toxic components many fold. Thus, such extraction of botanicals that may contain hazardous constituents should be a cause for concern unless there is credible evidence to the contrary. Such procedures underlie the process by which natural products chemists isolate specific bioactive substances from plant material for further purification and identification. Given the potential for extraction to impact the constituents consumed, it is not appropriate to assume that safety of an aqueous extract of a botanical indicates that alcoholic extracts (or other organic solvent extracts) of the same botanical are not of concern.

6  

Although most of the toxins are small molecules (< 1,000 Daltons), there are a number of high-molecular-weight proteins, particularly lectins, that are extremely toxic. For example, ricin, occurring in the castor bean (Ricinus communis), is one of the most toxic substances known with a minimal lethal dose intraperitoneal injection of 1 ng/g body weight in mice (O’Neil et al., 2001).

7  

This statement must be tempered by the fact that many alkaloids occur in plants as water-soluble salts and others have high levels of hydroxylation that confer water solubility on them.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
×
Historical/Traditional Use

Certain plant families, while being major sources of food plants, also contain some of the most toxic plant species (see discussion in Appendix C). Species of Fabaceae and Solanaceae that are toxic have been established (probably a reflection of extensive investigations of the secondary metabolite compositions of these families because they are primary food sources), but even some species that contain toxins are used as food plants. For example, plants in the following families are used as foods: Liliaceae (e.g., onion, garlic, asparagus), Apiaceae (e.g., carrots, celery, parsnips), Brassicaceae (e.g., green leafy vegetables), Fabaceae (e.g., peas and beans), and Solanaceae (e.g., potatoes, tomatoes, eggplant). In most cases, the toxin tends to be concentrated in specific plant parts (e.g., potato sprouts) that are not consumed as conventional foods (Drager et al., 1995). Levels in plant parts conventionally eaten as foods are generally known and, in some cases, regulated (e.g., steroidal alkaloids in potatoes); when present, the levels are sufficiently low that they can be metabolized and excreted without adverse effects.

Some general principles apply when considering the relevance of historical or traditional use of a plant. It is important to consider the points outlined in the previous section, particularly the importance of plant part and whether the dietary supplement ingredient preparation will allow excessive amounts to be consumed or will concentrate toxins (in capsules vs. teas, or alcoholic vs. aqueous extracts, for example). A dissimilar amount of ingested toxin could result from changes, such as a switch from cooked to raw consumption, inclusion of plant parts not traditionally consumed, excessive use of one particular vegetable foodstuff, preparation of infusions or extracts, or ingestion of plant preparations historically only applied externally. For example, plant foods are typically consumed after cooking by either dry heat or boiling in water, a process that often destroys toxins because they are thermally labile, hydrolyzed, or extracted.

That particular concern should arise when nontraditional or excessive levels of plant parts are consumed can be illustrated by several examples. Increased consumption of potato skins without the flesh, because nutrients are generally considered more concentrated in this part, may lead to ingestion of high levels of glycoalkaloids and glycosidase-inhibitory calystegines known to be concentrated in the skin, which can seriously affect digestive processes (Asano et al., 1997). Similarly, excessive use of vegetables of the family Brassicaceae can result in toxic effects due to isothiocyanates and other hydrolytic products from glucosinolates, which can cause goiter and a general inhibition of iodine uptake by the thyroid. Internal consumption of plants historically used externally, as with comfrey (Symphytum spp.), a pyrrolizidine alkaloid-containing plant, is especially relevant. Medieval

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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herbalists prescribed the use of comfrey as a poultice, but more recent use as a salad vegetable or tea has resulted in evidence of liver damage (Coulombe, 2003).

In summary, plant foods and spices, when consumed in a conventional manner, can generally be assumed to be safe if the same plant part is consumed, but caution should be applied to restrict higher than conventional ingestion of those known to have some degree of toxicity. Similarly, the assumption of safety may or may not apply to plants prepared differently. Of course, not all foods are safe for all persons. If adverse events are reported, then the level of concern should be higher. Most plant species containing overtly toxic compounds are not generally consumed, and consequently there will be no normal food intake data available. For plants that are not commonly consumed by humans, the best data as to adverse effects will come from observations and/or studies with herbivorous animals (see Chapter 5).

Summary of Chemical Components and Related Botanicals

When considering the safety of a botanical dietary supplement ingredient, information about its chemical constituents may provide important clues as to the potential toxicities of the substance. Similarly, consideration of related plant species, especially those in the genera listed in Table 6-1, can provide information about chemical constituents that may be present in the ingredient, as well as toxicities associated with these chemicals. Some information about the potencies is also provided in Appendix C. The concept that it is important to consider chemical constituents and related plants of possible concern is summarized in the guiding principle at the beginning of this section: it is appropriate to consider risk by considering “… whether the plant source of the botanical dietary supplement is itself a toxic plant or is taxonomically related to a known toxic plant.” Several corollary guiding principles are important to remember as individual supplements are considered:

  • Plant foods and spices consumed in a conventional manner can generally be assumed to be safe if the same plant part is consumed, but caution applies to higher than conventional ingestion of those known to have some degree of toxicity.

  • Alkaloid-containing plants should always be suspected of possibly being pharmacologically active and should be considered as a risk factor.

  • Unless there is evidence to the contrary, the assumption should be that all parts of a plant containing toxins pose a risk unless there is credible evidence suggesting that dangerous levels of toxins are not present in the part marketed as a dietary supplement.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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  • If production of a hazardous phytochemical in a botanical appears to be particularly sensitive to environmental and growth conditions, then it may be appropriate to consider its use in dietary supplements as a risk unless quality control or other actions are implemented to assure that toxic levels of compounds are not reached in raw materials or finished products.

  • Preparation affects toxicity. Materials that are traditionally consumed in cooked form may not have the same safety profile as in uncooked form, compounds that are concentrated or otherwise altered by the method of preparation will present a hazard that is of greater concern than for unprocessed material, and knowledge about the safety of one plant preparation should not be applied prima facie to different preparations of the same plant.

  • Particular concern should arise when nontraditional or excessive levels of plant parts are consumed. There may be a tendency to consume more of the plant material in an encapsulated form than if it were used in its “native” form.

  • Extraction of plant material with alcohol or aqueous alcohol, in which low-molecular-weight compounds are generally very soluble, will likely concentrate toxic components several fold. Thus, for botanicals containing toxic compounds, a shift from aqueous to alcoholic extracts should be a cause for concern unless there is credible evidence to mitigate this concern.

OTHER APPROACHES FOR CONSIDERING STRUCTURAL SIMILARITY TO KNOWN CLASSES OF TOXIC COMPOUNDS

GUIDING PRINCIPLE: Compounds that are similar in structure may have similar biological functions. If the chemical structure of a dietary supplement is known, but additional insight into the biological activity is needed, then it is scientifically appropriate to consider the information about the biological activity of structurally related substances and the general knowledge about adverse effects associated with toxicophores.

The physical–chemical properties and biological effects of a substance are derived from its chemical structure. If the chemical structure of a dietary supplement is known, but additional insight into the biological activity is needed, then it is scientifically appropriate to consider the information about the biological activity of structurally related substances. It is assumed that the biological effects of chemicals, including toxic effects, are implicit

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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in their molecular structures (referred to as toxicophores when they are associated with toxic effects). This concept is most clearly illustrated with the example of ephedra, which is considered by some scientists to have similar physiological actions, although less potent, to the chemically related substance amphetamine, as well as the recently banned pharmaceutical agent phenylpropanolamine (FDA, 2004; Furuya and Watanabe, 1993; Lake and Quirk, 1984).

Along these lines, FDA and other agencies have developed chemical structure classes of concern to describe well-known toxicophores. The structure of regulated chemicals (e.g., pesticides or food additives) is compared with structural classes of concern to predict which may cause adverse effects. For ingredients regulated by premarket approval, structures of higher concern classes lead to a requirement for particular studies to provide additional information about the likelihood of adverse effects occurring in humans. The structural class approach is discussed in Box 6-4 and in the FDA Redbook II (OFAS, 2001). When the structure of a dietary supplement ingredient or its constituents belongs in one of the higher classes of concern identified via the Structure Category Assignment, it should be considered as a potential risk if mitigating information suggesting other-

BOX 6-4
Chemical Relatedness (Structure Category Assignment)

In the United States, the Food and Drug Administration, Office of Food Additive Safety, has detailed the value of considering chemical relatedness in the process of assessing the potential of an unknown agent to cause undesirable adverse effects upon consumption in sufficient dosages (see Redbook II). This document proposes that it is reasonable to expect that the structure and associated physiochemical properties of a compound play an important role in its toxicity. Four variables for consideration in structure activity relationship (SAR) modeling are as follows:

  • Topological parameters

  • Geometric parameters

  • Electronic parameters

  • Physiochemical parameters

Thus chemicals with unknown toxicological properties may be assigned to a chemical structure category based on the presence or absence of chemical groups that have been associated with certain types of toxicity. Moreover, the guidelines provided in Redbook II allow for the assignment of an unknown chemical to an initial Concern Level for potential adverse toxic effects (OFAS, 2001). These SAR indicators should be utilized when evaluating dietary supplement ingredients.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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wise does not exist. The rationale behind this recommendation is that the dietary supplements with structures of concern are no less likely to produce adverse effects than other ingested substances. Ideally, the use of information about the structural classes of concern will provide guidelines for manufacturers or other scientists to study the ingredient’s safety in more depth.

Based on the understanding that biological effects are implicit to molecular structures, computational programs have been developed to predict the biological activity of less-characterized chemicals by comparing their chemical structures with other well-characterized compounds. Computer programs designed to assist in predictive toxicology are useful in predicting the potential propensity for a chemical to cause particular effects. For example, The Open Practical Knowledge Acquisition Toolkit program (AIAI, 2003) uses chemical structures and a variety of models to estimate carcinogenicity and teratogenicity, among other toxicological endpoints, and is used by the Cosmetic Industry Review in setting priorities for review. An endorsement or comparative evaluation of individual programs is beyond the scope of this report, but these types of programs in general are believed to have value in providing insight into the potential for a dietary supplement ingredient to demonstrate toxicological outcomes, especially if there is a paucity of experimental or other data relevant to the ingredient’s safety.

Computational prediction is most useful for predicting biological activities of pure compounds because it is possible to circumvent the multitude of variables that influence toxicological endpoints, such as the presence of unknown chemicals, the animal species, strains, experimental conditions, and other factors. Even then, however, care must be exercised in selecting data that may be considered valid. Additional complexities, even vagaries, such as metabolic transformation and the multitude of modulating factors that determine a biological outcome under prescribed conditions, may obviate any meaningful predictive conclusion.

Moreover, other confounding factors are the often unique susceptibilities of individuals in animal and human populations that are dependent on complex genetic, environmental, and lifestyle factors. Even more difficult is the reliability of predictions of toxic response where there are effects of simultaneous exposures to a variety of compounds. Even the common example in humans of combining alcohol and tobacco smoke can exercise a striking influence on the toxic manifestations of another (test) material (Izzotti et al., 1998).

In summary, the understanding that toxic effects result from molecular structures that act on biological targets provides a good rationale for comparing chemical structures of a dietary supplement ingredient with other chemical structures to predict possible toxicities. Nonetheless, the

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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practicalities of how to make such comparisons in a systematic way and the limitations inherent in systematic prediction software may limit the usefulness of this approach, as may the important fact that small changes in chemical structure can result in major changes in physiological activity.

CONSIDERATION OF SUBSTANCES RELATED TO ENDOGENOUS SUBSTANCES

GUIDING PRINCIPLE: Dietary supplement ingredients that are endogenous substances or may be structurally or functionally related to endogenous substances should be evaluated to determine if their activities are likely to lead to serious effects. Considerations should include the ingredient’s ability to raise the steady-state concentration of biologically active metabolites in tissues and whether the effect of such increases is linked to a serious health effect.

A number of dietary supplement ingredients may be structurally or functionally related to endogenous substances. Such substances include hormones, metabolites and their precursors, and ingredients created as mimetics of these substances (see Table 6-2 for examples). Any safety issues of ingredients related to endogenous substances are based on the extent to which the ingredient’s similarity to an endogenous substance alters homeostasis. Concern about one of these ingredients is warranted when certain characteristics or qualities are present, as discussed below.

Relevant Characteristics

Physiologically relevant amount of ingredient ingested: It is important to consider whether the ingredient is delivered to potential sites of action at a concentration that is physiologically relevant. For example, a supplement ingredient ingested at an amount that is clearly a small fraction of the amount typically provided in the diet is less likely to pose a risk because the likelihood of physiological impact is low.

Concentration at the site of action that can cause harm: The next consideration is whether the substance reaches the site of action at a concentration that can cause harm, which is largely determined by the substance’s bioavailability, rate of metabolism, and excretion. For example, dietary supplement ingredients for which evidence suggests negligible uptake from the gastrointestinal tract would be unlikely to pose a risk beyond local effects, such as gastrointestinal upset. In contrast, ingredients that may result in concentrations of endogenous substances above the normal

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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TABLE 6-2 Examples of Dietary Supplement Ingredients Related to Endogenous Substances

Hormones and Ingredients Containing Hormones or Precursors

Lipids and Related Ingredients

Metabolites and Cofactors

Othersa

Androstenediol

Conjugated linoleic acid

S-Adenosyl-L-methionine

Chondroitin sulfate

Androstenedione

Docoshexaenoic acid

 

Collagen

Dehydroepiandrosterone (DHEA)

(DHA)

Creatine

Enzymes

Glandulars

Eicosapentaenoic acid

α-Lipoic acid

Glucosamine

Human growth hormone

(EPA)

NADH

Indole-3-carbinol

Melatonin

Inositol hexaphosphate

Pyruvate

Probiotics

Pregnenolone

Phosphatidyl choline

Phosphatidyl serine

Nucleic acids

 

a In addition to this list, there are also many other plant and animal constituents known to convert to biologically active subs tances similar to endogenous substances.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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range at the site of action are worthy of further consideration, because of the potential for their activities to be adverse.

Having determined whether an ingredient is likely to be absorbed from the gut at a concentration that could alter cellular, biochemical, or biological activities, the next issue to consider is whether the compound is readily metabolized or degraded to an inactive metabolite. For example, effects on endpoint cells and tissues are unlikely if there are efficient mechanisms for metabolizing these compounds into inactive compounds.

Sensitivity of the target system to variation in the endogenous substance: An important question to ask about endogenous substances is whether a homeostatic regulatory system would attenuate biological effects that could otherwise occur. If the target system is one that is not tightly regulated by feedback or other mechanisms to maintain homeostasis, then there is greater likelihood of potential risk.

Example

Hormones are an illustrative example for how some ingredients related to endogenous substances can be evaluated. Exogenous hormones can be potent substances often used clinically as pharmaceuticals to treat specific deficiency states (e.g., insulin to treat diabetes, thyroid stimulating hormone to treat hypothyroidism, human growth hormone to treat dwarfism) in order to achieve physiological homeostasis. Use of dietary supplements containing hormones, hormone precursors, or hormone mimetics known to be highly potent raises the possibility of significant and substantial harm unless there is demonstrated hormonal insufficiency.

CONSTITUENTS FUNCTIONALLY RELATED TO KNOWN CLASSES OF TOXIC COMPOUNDS

GUIDING PRINCIPLE: When data (i.e., in vitro or animal data) suggest that a dietary supplement constituent targets a receptor, enzyme, or other biological target in a manner similar to a compound known to be toxic, concern is warranted, especially if the dietary supplement constituent is known to reach the biological target in a relevant concentration.

Compounds that appear to be structurally dissimilar may nonetheless affect the same biological targets or have the same mechanism of action and thus result in the same downstream adverse health effect. Thus, if data strongly suggest that similar biological activity or mechanisms of action

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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exist between an ingredient and a substance known to be dangerous, there is scientific merit in considering whether similar adverse health effects might also occur. This is especially true if the ingredient or its relevant metabolites are bioavailable at the target site.

Although the actual data for such consideration will fall into the categories of data described in the previous chapters (in vitro, animal, or human data), the consideration of functional relatedness is described here rather than in the other chapters because (1) the concept applies to all types of data and (2) because the concept is similar to the concept of considering substances related in other ways (either structurally or, for botanicals, taxonomically). That is, a safety evaluation should consider the relationship between the dietary supplement ingredient in question and compounds known to be toxic. This type of information may be most useful in assessing the safety of a dietary supplement ingredient for which chemical constituents are not known or, for botanicals, when not much is known about the plant genus.

Functionally related substances may have similar actions in vitro, such as genetic effects or effects on cellular processes (e.g., enzymatic effects, effects on intracellular cell signaling). One example of such functional relatedness illustrated in Chapter 11 is saw palmetto and the drug finasteride. Finasteride is considered unsafe for consumption during pregnancy because of effects on male genitalia development (Bowman et al., 2003; Clark et al., 1990, 1993; Kurzrock et al., 2000). This effect is due to inhibition of the 5-α-reductase enzyme, which is important in testosterone production (Anderson and Clark, 1990; Prahalada et al., 1997). Saw palmetto also inhibits 5-α-reductase, as shown in in vitro experiments (Bayne et al., 1999), and thus would be considered as functionally related to finasteride. Thus, in the absence of mitigating data suggesting that saw palmetto does not also lead to male genitalia development problems, it would be scientifically appropriate to consider saw palmetto as a risk for consumption by pregnant women.

When considering which substances a given dietary supplement may be functionally related to, the purported mechanism of action of the ingredient should be considered. For example, shark cartilage has been referred to as an angiogenesis inhibitor. If angiogenesis inhibition is considered as dangerous, or angiogenesis inhibiting drugs are only used with caution by pregnant women because of this mechanism, then it would be appropriate to consider whether shark cartilage is indeed a risk for the same reason.

When evaluating whether functional relatedness to other chemicals provides helpful information about the safety of a dietary supplement ingredient, it is very important to consider that overt expression of toxicity is dependent on exposure (i.e., amount ingested or dose). When animal or human data do not exhibit the toxic effects predicted based on functional relatedness, then it is necessary to consider whether the possible effects

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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would not have been detectable, even if they did occur (such as would be expected for genetic effects or latent effects) (see also see “lack of effects” discussion in Chapter 10). Similarly, the amount of dietary supplement ingredient necessary to produce the effect in humans should be compared to the amount actually consumed.

In summary, if there is information about the mechanism of action of a substance suggesting that it exerts action similar to other classes of substances that are either considered dangerous or restricted in their use, then it may be appropriate to extrapolate such information to the dietary supplement ingredient, especially if little other information about the action of the supplement ingredient is available.

SUMMARY

This chapter emphasizes the importance and scientific appropriateness of including information about related substances when considering the safety of a particular dietary supplement ingredient. For botanicals, the taxonomic and chemical relationships questions can be considered in parallel. Although evidence from one relationship alone may be sufficient to cause an awareness of significant risk, a higher level of concern arising from consideration of both relationships will amount to compelling evidence that the risk has to be seriously examined.

When a dietary supplement contains a known toxic substance, the hazard of ingestion must be assumed to be high unless mitigated by information about dose. Similarly, if the complete toxicity information about a given compound is not known, but it falls into a structural class of known toxins, then that compound is also likely to be a risk unless there is mitigating information about bioavailability or exposure levels.

Table 6-3, the relative spectrum of concern for relatedness information, provides general guidelines about the relative amount of concern for example scenarios using the different types of relatedness information. The information in the right-hand column suggests a significant risk to public health even in the absence of direct human data documenting adverse effects caused by the dietary supplement ingredient. In many situations like those described in the left-hand column, a conclusion of imminent risk may require corroboration with other types of information.

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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TABLE 6-3 Relative Spectrum of Concern for Relatedness Information

Type of Information

Increasing Concern

Botanical chemical constituents of concern

Plant contains constituents that are known to be toxic to humans, but the constituents are commonly consumed in similar amounts in conventional food products

Plant contains constituents that are known to be toxic to humans at low concentrations, but the concentration of these substances in the plant part used for the supplement is not characterized

OR

Plant part used for the supplement contains constituents that are toxic to humans, but there is credible reason to believe that the constituent may not cause serious adverse effects at the amount typically ingested

Plant contains constituents that are known to be toxic to humans at low concentrations and the concentration of substances found in the plant part used for formulating the supplement has been characterized

Taxonomic relationship to other botanicals of concern

In a plant family that contains known toxic genera, but the supplement is not in a genus known to be toxic

AND

It has a history of use as a food in a preparation and method similar to its current use

Same genus known to be toxic to humans or animals

OR

In a plant family that is known to contain toxic plants but not in a genus known as a food plant

OR

Same species as a known toxic plant that is not ingested as a food

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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Type of Information

Increasing Concern

 

 

In a plant family that is known to contain toxic plants,and in a genus that may have a history of food use, but supplement is either a concentrated extract or from a different plant part than is ingested as a food

 

Chemical structure

Structurally similar to, or likely contains, a chemical compound known to be toxic to humans or animals when ingested in high doses

Supplement contains chemical constituent known to be toxic to humans or animals

Supplement contains chemical constituent known to be toxic at very low doses to humans

Endogenous substances or mimetics of endogenous substances

May result in tissue concentrations that would be expected to cause biological effects (either because homeostasis is disrupted or because the substance has potent biological activities), but the seriousness of the biological effect is not definite

Results seen in tissue concentrations that would be expected to cause biological effects (either because homeostasis is disrupted or because the substance has potent biological activities), but the seriousness of the biological effect is not definite

Results seen in tissue concentrations that would be expected to cause biological effects (either because homeostasis is disrupted or because the substance has potent biological activities) that are considered serious

Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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Suggested Citation:"6 Categories of Scientific Evidence--Information About Related Substances." Institute of Medicine and National Research Council. 2005. Dietary Supplements: A Framework for Evaluating Safety. Washington, DC: The National Academies Press. doi: 10.17226/10882.
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The growing consumer interest in health and fitness has expanded the market for a wide range of products, from yoga mats to the multiple dietary supplements now on the market. Supplements are popular, but are they safe? Many dietary supplements are probably safe when used as recommended. However, since 1994 when Congress decided that they should be regulated as if they were foods, they are assumed to be safe unless the Food and Drug Administration can demonstrate that they pose a significant risk to the consumer. But there are many types of products that qualify as dietary supplements, and the distinctions can become muddled and vague. Manufacturers are not legally required to provide specific information about safety before marketing their products. And the sales of supplements have been steadily increasing—all together, the various types now bring in almost $16 billion per year. Given these confounding factors, what kind of information can the Food and Drug Administration use to effectively regulate dietary supplements? This book provides a framework for evaluating dietary supplement safety and protecting the health of consumers.

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