TABLE 3-5 Tools Used to Assess Spinal Cord Injuries in Laboratory Animals
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Functional recovery |
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Basso, Beattie, and Bresnahan (BBB) scale, an open-field locomotor test for rats |
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Is based on 5-point Tarlov scale |
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Analyzes hind-limb movements of a rat in an open field |
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Is a 21-point scale used to assess locomotor coordination |
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Rates parameters such as joint movements, the ability for weight support, limb coordination, foot placement, and gait stability |
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Small changes in tissue correlate to large changes on the scale |
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Assesses walking, not other movements requiring coordinated spinal cord activity |
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Does not assess pain, bowel, bladder, or sexual function |
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Basso Mouse Scale (BMS), an open-field locomotor test for mice |
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Is an adaptation of rat BBB scale to examine the recovery of hind-limb locomotor function |
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Assesses walking, not other movements requiring coordinated spinal cord activity |
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Does not assess pain, bowel, bladder, or sexual function |
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Neuronal activity assessment by electrophysiology |
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Assesses MEPs or SSEP |
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Stimulates corresponding cortical areas of the brain and records response in target nerves to see if connections are still functional |
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Correlates to impairment of locomotor activity |
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Is noninvasive |
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Neuronal activity may not correlate with functional changes |
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Hard to assess subtle but critical improvements to circuitry |
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Does not directly assess pain, bowel, bladder, or sexual function |
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Forepaw withdrawal |
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Investigates recovery of heat perception |
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The forepaw is placed on a heat block and the time that it takes for the animal to withdraw it is measured |
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Forepaw withdrawal requires motor function |
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Does not assess pain, bowel, bladder, or sexual function |
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Directed forepaw reaching |
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Looks at coordinated limb and muscle movement |
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Requires rats to reach under a barrier and pick up food with forepaws |
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Limited scale for assessment |
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Does not assess pain, bowel, bladder, or sexual function |
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Morphological assessment of recovery |
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Histology |
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Is used to look at the morphology of axons and assess the degree of tissue sparing, injury, and recovery |