The cargo space of conveyances for transport of animals must be designed, constructed, and maintained in a manner that protects the health and well-being of each animal and ensures its safety and comfort.
The cargo space must have supply of air that is sufficient for normal breathing of all animals, and entry of engine exhaust must be prevented.
Enclosures must be positioned in the cargo space in a manner that provides protection from the elements, allows each animal enough air for normal breathing, and allows for the removal of animals in the event of an emergency.
During transport, auxiliary ventilation must be used in the cargo space when the temperature reaches 75°F. The temperature may not exceed 85°F or fall below 45°F except when rabbits are being transported and a certificate of acclimation to lower temperatures has been provided.
The cargo space must be kept clean, and animals may not be transported with any items that may be expected to cause harm to them.
During surface transportation, animals must be observed at least once every 4 hr to ensure sufficient air and acceptable ambient temperatures. If an animal is in obvious physical distress, the operator must obtain appropriate veterinary care as soon as possible.
During air transportation, animals must be observed at least once every 4 hr unless the cargo area is not accessible during flight, in which case animals must be observed whenever they are loaded and unloaded to ensure sufficient air and acceptable ambient temperatures. If an animal is in obvious physical distress, veterinary care must be provided as soon as possible.
During transportation, animals shall not be removed from their enclosure unless it is to be placed in another enclosure or facility that conforms to the applicable AWA regulations.