TABLE 4-1 Number and Type of Specimens Collected from AFHS Subjects by Year and Cycle

Year

1982

1985

1987

1992

1997

2002

Total # of samples

Exam Cycle

1

2

3

4

5

6

Blood serum

 

# of samples

15,883

6,927

4,588

8,932

8,484

5,853

50,667

# of vials

5–10

3

2

4

4

3

 

Volume (ml)

2

5

10

7

10

10

 

Whole blood

 

# of samples

 

19,510

19,510

# of vials

10

 

Volume (ml)

1

 

Urine

 

# of samples

2,269

2,309

2,294

 

6,872

# of vials

1

1

1

 

Volume (ml)

50

50

10

 

Semen

 

# of samples

8,845

 

8,845

# of vials

1–10

 

Volume (ml)

1

 

Adipose tissue*

 

# of samples

 

313

 

313

Weight (g)

10

 

*A sample of approximately 10 g was extracted by liposuction (AFHS, 2000).

SOURCE: J. Robinson, Air Force Health Study, personal communication, September 21, 2005.

test plans—created by SAIC—for Cycles 2–6.2 A detailed protocol is not available for Cycle 1. Cycle 1 laboratory methods are discussed in the original protocol (AFHS, 1982); they are also briefly described in the Cycle 2 biomedical test plan (SAIC, 1985) where shift in laboratory techniques or methods between Cycles 1 and 2 is discussed.

Table 4-1 provides summary counts of the types and quantities of biological specimens collected in each cycle. Sera were stored in all six cycles and aliquot volumes varied by cycle ranging from 2 to 10 ml per aliquot. The number of aliquots per subject also varied ranging from 1 to 10 vials in Cycle 1, 3 vials in Cycles 2 and 6, 2 vials in Cycle 3, and 4 vials in Cycles 4 and 5. In Cycle 6, ten 1-ml aliquots of whole blood were also stored with ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. There was no separation of white blood cells.

2  

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