. "Appendix C: Agenda." Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collections: Workshop Summary. Washington, DC: The National Academies Press, 2006.
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Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collection: Workshop Summary
Appendix C
Agenda
Keck Center
National Academies
500 Fifth Street, N.W.
Washington, D.C.
May 8, 2006
8:30-9:00 a.m.
Opening Remarks and Introduction
Ann Bucklin and Donald Crothers, Cochairs
8:30-8:45
Purpose of the Workshop
Scott Miller
Session 1: State of DNA in Biological Samples after Exposure toFormalin
What is the state of preservation of DNA in the presence of formalin? Are the DNA chains intact or broken? Does formalin denature DNA or is it the process of extraction that is fragmenting the DNA? Are the nucleotides at each site being preserved or altered?
How can the physical and chemical states of the DNA-formalin cross-linkages be better characterized? What additional information on these cross-linkages is needed?
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Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collection: Workshop Summary
Appendix C
Agenda
Keck Center
National Academies
500 Fifth Street, N.W.
Washington, D.C.
May 8, 2006
8:30-9:00 a.m.
Opening Remarks and Introduction
Ann Bucklin and Donald Crothers, Cochairs
8:30-8:45
Purpose of the Workshop
Scott Miller
Session 1: State of DNA in Biological Samples after Exposure to Formalin
What is the state of preservation of DNA in the presence of formalin? Are the DNA chains intact or broken? Does formalin denature DNA or is it the process of extraction that is fragmenting the DNA? Are the nucleotides at each site being preserved or altered?
How can the physical and chemical states of the DNA-formalin cross-linkages be better characterized? What additional information on these cross-linkages is needed?
Moderator: Donald Crothers
Rapporteur: Timothy O’Leary
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Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collection: Workshop Summary
9:00-10:00
Participant Brieflngs
Participants share their knowledge of and experience with assessing the condition of DNA in biological samples flxed in formalin
10:00-10:30
Open Discussion
10:30-10:45
Break
10:45-11:30
Open Discussion
Session 2: Optimization of DNA Sequence Information from the Samples
What new chemical and physical methods for DNA extraction should be tested, beyond those that have already been applied to formalin-flxed tissue?
In what ways and to what extent can fragmented DNA be repaired physically and chemically after extraction from formalin?
Moderator: Ann Bucklin
Rapporteur: Charles Cantor
11:30-12:30 p.m.
Participant Brieflngs
Participants share their knowledge of and experience with optimization of DNA sequence information from formalin-flxed samples
12:30-1:30
Lunch
1:30-3:45
Open Discussion
3:45-4:00
Break
Session 3: Bioinformatics for Reconstructing DNA Sequences
Can bioinformatics techniques be used to reconstruct the original sequence in silico from the DNA fragments recovered from formalin?
Moderator: Christoffer Schander
Rapporteur: Neil Hall
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Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collection: Workshop Summary
4:00-4:20
Participant Brieflngs
Participants share their knowledge of and experience with bioinformatics for restructuring DNA sequences
4:20-5:20
Open Discussion
5:20-5:30
First-day Summation
May 9, 2006
Session 4: The Path towards Effective Retrieval of Genomic Information from Formalin-Fixed Samples
Moderators: Donald Crothers and Ann Bucklin
8:30-9:15 a.m.
Summary of Sessions 1-3
Timothy O’Leary, Charles Cantor, and Neil Hall, Rapporteurs
9:15-10:30
Open Discussion
10:30-10:45
Break
10:45-11:45
Open Discussion (cont’d)
11:45-12:00
Workshop Summation
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Path to Effective Recovering of DNA from Formalin-Fixed Biological Samples in Natural History Collection: Workshop Summary
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