. "APPENDIX A PROPOSALS RECOMMENDED FOR FUNDING." Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008. Washington, DC: The National Academies Press, 2008.
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
APPENDIX A: PROPOSALS RECOMMENDED FOR FUNDING
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
HLB proposals recommended for funding
122
Falk
Controlling HLB by controlling psyllids with RNA intereference
$198,274
3
$602,507
$198,274
3
$602,507
$198,274
236
Yamamoto
Can insecticides and mineral oil avoid transmission of Candidatus Liberibacter asiaticus by Diaphorina citri?
$39,275
2
$78,000
$39,275
2
$78,000
$237,549
21
Brown
The citrus psyllid transcriptome and time course differential gene expression in Ca. Liberibacter-infected/free whole psyllids and organs
$450,438
3
entry blank
$165,000
3
$495,000
$402,549
Budget interpreted from proposal, assuming entries on budget page are in error.
79
Gowda
Development of sensitive, non-radioactive and rapid tissue blot diagnostic method for large-scale detection of citrus greening pathogen
$41,040
2
$82,500
$41,040
2
$82,500
$443,589
Committee expressed some concern about sensitivity of the tissue blot method.
204
Spann
Strategies to minimize growth flushes of mature citrus trees with pruning practices and plant growth regulators to reduce psyllid feeding
$149,238
3
$450,000
$137,238
3
$411,714
$580,827
72
Gmitter
Assessment of the HLB Resistance and Tolerance in Citrus and Its Relatives
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OCR for page 11
Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
APPENDIX A:
PROPOSALS RECOMMENDED FOR FUNDING
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
HLB proposals recommended for funding
122
Falk
Controlling HLB by controlling psyllids with RNA intereference
$198,274
3
$602,507
$198,274
3
$602,507
$198,274
236
Yamamoto
Can insecticides and mineral oil avoid transmission of Candidatus Liberibacter asiaticus by Diaphorina citri?
$39,275
2
$78,000
$39,275
2
$78,000
$237,549
21
Brown
The citrus psyllid transcriptome and time course differential gene expression in Ca. Liberibacter-infected/free whole psyllids and organs
$450,438
3
entry blank
$165,000
3
$495,000
$402,549
Budget interpreted from proposal, assuming entries on budget page are in error.
79
Gowda
Development of sensitive, non-radioactive and rapid tissue blot diagnostic method for large-scale detection of citrus greening pathogen
$41,040
2
$82,500
$41,040
2
$82,500
$443,589
Committee expressed some concern about sensitivity of the tissue blot method.
204
Spann
Strategies to minimize growth flushes of mature citrus trees with pruning practices and plant growth regulators to reduce psyllid feeding
$149,238
3
$450,000
$137,238
3
$411,714
$580,827
72
Gmitter
Assessment of the HLB Resistance and Tolerance in Citrus and Its Relatives
$50,000
3
$160,000
$50,000
3
$160,000
$630,827
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
114
Keyhani
Development of Asian citrus psyllid, Diaphorina citri, tissue culture cell lines
$62,860
1
$62,860
$80,000
1
$80,000
$710,827
Recommend budget increase to facilitate distribution and use of cell lines.
78
Gottwald
Efficacy of interplanting citrus with guava as a control strategy for Huanglongbing
$323,077
2
$1,198,741
$270,000
2
$540,000
$980,827
16
Briansky
Alternative Hosts of HLB to Assist in Disease Management
$205,000
3
$615,000
$205,000
3
$615,000
$1,185,827
Change in select agent status should reduce costs.
40
Damsteegt
Psyllid mediated completion of pathogenicity tests (Koch’s Postulates) with a pure culture of the associated Huanglongbing causal bacterium
$175,000
2
$350,000
$75,000
2
$225,000
$1,260,827
Budget reduced unless need for Brlansky’s participation can be justified; funding for year 2 requires completion of Koch’s postulates in year 1.
145
Moore
Evaluate Differences in Response to HLB by Scions on Different Rootstocks
$55,000
3
$165,000
$55,000
3
$165,000
$1,315,827
71
Gmitter
International citrus genome consortium (ICGC): Providing tools to address HLB and other challenges
$1,300,000
3
$2,500,000
$800,000
3
$1,600,000
$2,115,827
Committee considered budget request to be excessive for the activities proposed.
95
Hartung
Preparation of antibodies against Candidatus liberibacter asiaticus
$242,540
3
$714,000
$195,540
3
$564,000
$2,311,367
Annual support for consultants Briansky and Damsteegt reduced $50K per year ($150K total). Pysllid rearing & infectivity not well described. The recommended budget was reduced to bring it in line with the expected efforts in the various laboratories involved.
77
Gottwald
Epidemiology and disease control of HLB
$295,251
4
$1,198,741
$160,000
3
$480,000
$2,471,367
Recommend employing US-based postdoc in place proposed UK postdoc, salary reduced from 190K to 50K; the 4 yr term requested is not allowed by the
45
Dawson
Examine the response of different genotypes of citrus to citrus greening (Huanglongbing) under different conditions
$226,391
1
$226,391
$226,391
1
$226,391
$2,697,758
After much discussion, the budget as requested is considered to be reasonable.
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
217
Stelinski
Development of Effective Guava-based Repellent to Control Asian Citrus Psyllid and Mitigate Huanglongbing Disease Incidence
$177,369
3
$500,000
$114,000
3
$385,000
$2,811,758
Reduced funding is recommended because the investigators have published work using a GC with sulfur detection, so the need for this equipment did not seem justified, as they presumably have access to such equipment. Furthermore, the materials and technical support budgets appear excessive to conduct the described research, so the committee recommended funding for these areas be reduced (25% and 33% respectively).
126
Lopes
Factors influencing acquisition and inoculation of Candidatus Liberibacter spp. by Diaphorina citri
$63,200
3
$189,600
$63,200
3
$189,600
$2,874,958
163
Triplett
Integrative approaches to discover pathogenesis-associated proteins from the causal agent of citrus greening disease and build new diagnostic tools
$471,981
3
$930,311
$400,000
3
$860,000
$3,274,958
The cost and value of the subcontracts ($269,570 year 1) is questioned because the salary requests in the subcontracts seem high for the activities described, e.g., well established antibody cross absorption steps.
113
Kang
Correlative microscopic and molecular characterization of the microbiome in the citrus phloem tissue
$109,411
2
$223,082
$100,000
1
$100,000
$3,374,958
Budget was reduced based on the activities proposed; a second year of funding should be considered only following a first year result demonstrating application of the antibody.
214
Stelinski
Quantitative measurement of the movement patterns and dispersal behavior of Asian citrus psyllid in Florida for improved management
$101,631
2
$195,000
$101,631
2
$195,000
$3,476,589
179
Rouse
Cultural Practices to Prolong Productive Life of an HLB Infected Tree
$181,887
3
$290,000
$148,290
3
$222,808
$3,624,879
Committee recommends reducing the personnel budget by 25% for years one and two based on anticipated workflow.
162
Duan
Dissecting the Disease Complex of Citrus Huanglongbing in Florida
$473,512
2
$975,608
$250,000
2
$500,000
$3,874,879
Retain objectives 3 and 4 only; USDA primers should be among those applied in this research.
2
Albrigo
Characterize the roles of calfose and phloem proteins in citrus Huanglongbing (HLB) symptom development
$196,539
3
$518,610
$100,000
3
$260,000
$3,974,879
Retain objectives 1a and 2
184
Salyani
Evaluation and development of effective ultra low volume spray technologies for management of the Asian citrus psyllid
$99,058
2
$195,000
$99,058
2
$195,000
$4,073,937
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
38
Cox
Management of Pyslla in tree fruit crops using RNA interference
$106,915
3
$329,379
$106,915
1
$106,915
$4,180,852
One year of support for proof of concept to show if there is dimunition of expression due to application of RNAi.
203
Spann
Using physical and chemical property changes of citrus leaves as early indicators of HLB infection and effects of added plant nutrients
$164,631
3
$495,000
$164,631
1
$164,631
$4,345,483
One year of support for proof of concept that any spectral or other rapid measure without tissue extraction can reflect HLB specifically.
191
Schumann
intensively managed citrus production systems for early high yields and vegetative flush control in the presence of greening disease
$229,552
3
5600,000
$229,552
3
$600,000
$4,575,035
The effort described should required fewer personnel than requested: Committee recommends negotiation to a reduced budget.
144
Moore
Agrobacterium-mediated Genetic Transformation of Mature Citrus Tissue
$224,000
3
$648,000
$125,000
3
$375,000
$4,700,035
This project should be performed as a collaboration rather than using a Spanish consultant.
175
Rogers
Resistance and cross-resistance development potential in Asian citrus psyllid to insecticides and its impact on psyllid management
$150,315
2
$300,000
$75,000
2
$150,000
$4775,035
Fund only objectives 1 and 2.
215
Stelinski
Identification of psyllid attractants and development of highly effective trapping and attract-and-kill methods for improved psyllid control
$180,049
3
$500,000
$180,049
3
$500,000
$4,955,084
48
Dollet
Attempts to in vitro culture Candidatus Liberibacter asiaticus isolates in order to fulfil Koch’s postulates
$95,313
2
$167,378
$95,313
2
$167,378
$5,050,397
42
Dandekar
Identification and modeling of early responses to HLB infection to improve disease management
$381,636
1
$381,636
$250,000
1
$250,000
$5,300,397
Reduce expenses incurred at sites other than Davis; project will necessarily ramp-up, so expenses should be less than requested.
34
Cicero
Gross and fine structure localization of Liberibacter in citrus psyllid Diaphorina citri organs: elucidating the transmission pathway
$120,000
3
$289,620
$120,000
3
$289,620
$5,420,397
87
Grosser
Accelerating the Commercialization of Transformed Juvenile Citrus
$178,000
3
$534,000
$178,000
3
$534,000
$5,598,397
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
125
Lin
Development of SSR markers for detection, genotyping, phenotyping and genetic diversity assessment of Ca Liberibacter stains in Florida
$105,342
3
$264,087
$105,342
3
$264,087
$5,703,739
Genotyping vs phenotyping to characterize HLB strain severity is a key element to study in this proposal.
231
Wang
Characterize the microbiomes associated with Candidatus Liberibacter asiaticus infected citrus, psyllid, dodder, and periwinkle
$145,114
2
$259,000
$145,114
2
$259,000
$5,848,853
176
Rogers
Effects of nutrition and host plant on biology and behavior of the Asian citrus psyllid and implications for managing psyllid populations
$98,333
3
$300,000
$66,000
3
$200,000
$5,914,853
Retain objectives 2 and 3 only; objectives 1 and 4 are poorly developed and should be abandoned.
93
Hall
Pathogen-Vector Relations between Asian Citrus Psyllid and Liberibacter asiaticus
$213,434
3
$616,112
$112,000
1
$112,000
$6,026,853
One year for proof of concept of the feeding system; retain objectives 1 ($100K) and part of objective 2 ($12K) only
213
Stelinski
Development and optimization of biorational tactics for Asian citrus psyllid control and decreasing huanglongbing incidence
$99,778
2
$190,000
$90,000
2
$180,000
$6,116,853
Eliminate objective 3.
230
Wang
Genome sequencing of Candidatus Liberibacter asiaticus
$248,916
2
$429,600
$248,916
2
$429,600
$6,365,769
65
Gabriel
Genomic sequencing to closure of a curated Florida citrus greening strain of Candidatus Liberibacter asiaticus
$680,367
2
$892,027
$230,000
2
$460,000
$6,595,769
The budget is much larger than needed to accomplish the activities proposed.
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
196
Setamou
Coupling citrus flush management and dormant chemical spray as a strategy to control populations of Asian citrus psyllid
$56,794
2
$113,000
$56,794
2
$113,000
$6,652,563
5
Baldwin
Effects of HLB on quality of orange juice and identification of HLB-induced chemical signatures in fruit juice and leaves
$147,567
2
$234,634
$147,567
2
$234,634
$6,800,130
161
Powell
A Rapid Screening Process for Chemical Control of Huanglongbing
$75,730
2
$175,000
$75,730
2
$175,000
$6,875,860
129
Lu
Manipulating SA-mediated defense signaling to stimulate broad-spectrum resistance to HLB and other diseases in citrus
$184,897
3
$510,000
$184,897
1
$105,897
$7,060,757
Limited success of the proposed approach in other systems indicates that the proposed research is high risk; recommend one year funding for proof of concept; no justification is provided for the requested $79K consultant; recommended budget is reduced by $79K but might be restored to requested amount if consultant cost is properly documented.
67
Gmitter
Surviving HLB and canker genetic strategies for improved scion and rootstock varieties
$840,046
3
$2,400,000
$300,000
3
$900,000
$7,360,757
Cut personnel to 1 postdoc and 2 staff; collection and some other activities do not require a postdoc level appointee.
187
Schaad
Cultivation and Identification of the Causal Agent of Huanglongbing Disease of Citrus
$643,390
2
$1,195,400
$200,000
2
$200,000
$7,560,757
Recommend funding for $100K genome sequence analysis and $1 OOK culturing project but not for the screenhouse; satisfy Koch's postulates in Florida.
61
Filho
Diagnosis of Candidatus Liberibacter asiaticus in plant and vector based on molecular and serological approaches.
$32,500
2
$58,500
$32,500
2
$58,500
$7,593,257
Can get applied information on Ca Lam as well as Ca Las plus antiserum development for a small price.
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
149
Mou
Transferring Disease Resistance Technology from a Model System to Citrus
$100,000
3
$250,000
$100,000
1
$100,000
$7,693,257
The proposed research depends on citrus transformation and regeneration having sufficient throughpup and on results from non-citrus systems being transferable or relevant to citrus and therefore is high risk; recommend one year of funding for proof of concept for the proposed approaches.
132
Machado
Analysis of transcriptome of citrus infected with Ca. Liberibacter asiaticus and Ca. L. americanus.
$82,000
2
$82,000
$82,000
2
$82,000
$7,775,257
Applicant requested $82K for year one and $82K for the two year total; objectives need to be spelled out and transcriptomics methods more adequately described.
172
Roberts
Spatial and Temporal Incidence of Ca. Liberibacter in Citrus and Psyllids detected Using Real-time PCR
$90,120
3
$270,360
$90,120
3
$270,360
$7,865,377
Considered to be an good applicable proposal.
13
Borovsky
Control of the Asian citrus psyllid, Diaphorina citri Kuwayama with protease inhibitors and RNAi.
$196,401
3
$606,510
$196,401
1
$196,401
$8,061,778
Risky approach; one year of support for proof of concept recommended.
190
Schumann
Advanced control system for variable rate application of fertilizer and pesticide to trees in the presence of greening and canker
$29,846
1
$29,846
$29,846
1
$29,846
$8,091,624
Considered to be a highly applicable proposal.
7
Bassanezi
Comparative epidemiology of citrus huanglongbing (greening) caused by Candidatus Liberibacter asiaticus and Ca. L. americanus
$138,950
3
$185,650
$28,950
3
$75,650
$8,120,574
$110K equipment budget has not been justified properly; the Committee questions whether the equipment actually is needed for this proposed project; equipment must be specifically justified if the budget is to be restored to a level above the recommended amounts.
8
Bassanezi
Reduction of bacterial inoculum and vector control as strategies to management of citrus huanglongbing (greening)
$49,000
3
$174,000
$49,000
3
$174,000
$8,169,574
Committee expresses concern about the inadequate sampling planned in this proposed research; this proposal is not as meritorious as another on a very similar topic, but still may have a contribution to make.
90
Halbert
An effective trap for Asian citrus psyllid that can be used to monitor groves and plants for sale
$150,968
2
$302,000
$100,000
2
$200,000
$8,269,574
The personnel requests are excessive in the investigator-proposed budget.
14
Bowman
Development of Promising New Rootstocks and Scions for Florida Citrus
$200,953
3
$603,000
$150,000
3
$460,000
$8,419,574
The budget requests are considered to be excessive for the work proposed, but up to the original request could be restored with proper justification.
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
86
Graham
Does systemic acquired resistance (SAR) control HLB disease development?
$68,161
2
$132,223
$68,161
2
$132,223
$8,487,735
92
Hall
Asian Citrus Psyllid—Sampling, Biological Control, and Seasonal Profile of HLB in Adult Psyllids
$66,039
2
$128,934
$81,000
2
$162,000
$8,568,735
If this project is funded, the technician on the project should be full time.
206
Spreen
Long-Run Processed Orange Production and Price Impacts Associated with Citrus Greening in Florida and Sao Paulo, Brazil
$51,110
2
$102,000
$51,110
2
$102,000
$8,619,845
25
Burns
Combating symptom development in fruit from Huanglongbing-infected citrus trees: A transcriptomic, proteomic and metabolomic approach
$211,331
3
$631,615
$150,000
1
$150,000
$8,769,845
In the context of providing information that may promote disease mitigation, this proposal is considered to be high risk; Recommend funding for objectives 3, 4 and 6 only; the requested funding has been reduced accordingly and recommended for one year only to allow the investigator to collect data that may support continuation of the project.
232
Wang
Characterization the virulence mechanism of the citrus Huanglongbing pathogen Candidatus Liberibacter asiaticus
$171,468
3
$398,200
$160,000
1
$160,000
$8,929,845
Proposed project has potential to provide insight into HLB disease mechanism but is risky, justifying one year support for proof of concept
140
Marrs
Determining the Microbiome of Healthy and Infected Citrus Phloem Tissue, & Determining the Genome Sequence of Liberibacter
$349,012
2
$698,023
$225,000
1
$225,000
$9,154,845
Remove specific aim 3; one year of funding for proof of concept; second year of funding, if awarded, to be on completion of sequence.
88
Gurley
Engineering citrus for resistance to Liberibacter and other phloem pathogens
$113,042
3
$335,168
$113,042
1
$113,042
$9,267,887
Recommend one year of funding for proof of concept of the proposed approaches.
84
Graham
Transmission of HLB by citrus seed
$36,561
2
$73,123
$36,561
2
$73,123
$9,304,448
209
Stansly
Evaluation of Systemic Acquired Resistance Inducers Combined with Psyllid Control to Manage Greening in Infected groves.
$80,471
3
$241,413
$50,000
3
$150,000
$9,354,448
Reduced funding recommended based on activities proposed.
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Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
Citrus canker proposals recommended for funding
74
Gottwald
The importance of lesions of citrus canke on fruit
$69,848
1
$69,848
$69,848
1
$69,848
$69,848
233
Wang
Identification and characterization of critical virulence and copper resistance genes of Xanthomonas axonopodis pv. citri & related species
$190,200
3
$416,083
$190,200
3
$416,083
$260,048
186
Santra
Development of a novel nanoparticle/nanogel formulation for the prevention of citrus canker disease
$90,000
1
$90,000
$90,000
1
$90,000
$350,048
168
Ritenour
Pre-Grading Fresh Citrus for Canker Prior to Dumping on the Main Packingline
$34,709
2
$69,418
$34,709
2
$69,418
$384,757
202
Song
Engineering Resistance Against Citrus Canker and Greening Using Candidate Genes
$115,213
3
$330,396
$115,213
3
$330,396
$499,970
85
Graham
Survival of Xanthomonas citri ssp. citri (Xcc) to estimate risk of citrus canker transmission by infected fruit
$124,906
3
$245,812
$100,000
3
$245,812
$599,970
Biofilm investigation is unnecessary; however objective 2 is good.
81
Graham
Systemic acquired resistance (SAR) for control of citrus canker on young trees
$101,664
3
$304,992
$101,664
3
$304,992
$701,634
Recommendation is based on an approach worthy of pursuit but poorly described in this proposal.
76
Gottwald
Efficacy of citrus canker control strategies, leafminer interactions, and bacterial survival.
$237,472
3
$724,230
$187,472
3
$574,230
$889,106
Over half of the first year budget is devoted to a subcontract for research to be performed in Brazil, but the justification for the Brazilian budget is incomplete; budget recommendation is reduced with the expectation of partial or complete restoration should specific and reasonable justification be provided.
102
Horvath
Genetic Resistance to Citrus Canker conferred by the Pepper Bs3 Gene
$242,040
3
$586,747
$192,040
1
$192,040
$1,081,146
The first year budget seems to be excessive for the activities proposed; interspecific functionality of R genes is uncommon, so only one year of funding is recommended for proof of concept.
OCR for page 20
Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for all proposals, First year
Comments and Explanations of Recommended Changes to Budget
83
Graham
Canker management in Florida citrus groves: chemical control on highly susceptible grapefruit and early orange varieties
$134,974
3
$404,922
$100,000
3
$300,000
$1,181,146
Recommend deletion of objective 4.
OCR for page 21
Letter Report to the Florida Department of Citrus on the Review of Research Proposals on Citrus Greening, December, 2008
Proposal #
Applicant PI
Proposal title
First year funds requested (total direct)
Requested period of funding, yrs
Estimated full term requested budget
Committee recommended funds, yr 1
Committee recommended period of funding, yrs
Committee recommended funds, full term
Cumulative (running total) of recommended funding for ail proposals, First year
Comments and Explanations of Recommended Changes to Budget
Proposals recommended for funding under special consideration because they do not propose a research project (infastructure)
4
Stansly
Creation and Maintenance of an Online Citrus Greening Database
$25,153
1
$25,153
$25,153
1
$25,153
$25,153
158
Pena
Development of transformation systems for mature tissue of Florida commercial varieties, and strategies to improve tree management
$342,359
1
$342,359
$92,359
1
$92,359
$117,512
Is the requested greenhouse really needed? Committee members believe it is not and that the budget should be reduced accordingly but with an option for restoration should proper justification be provided.
108
Irey
Support for the Southern Gardens Diagnostic Laboratory
$109,306
2
$243,856
$109,306
2
$243,856
$226,818
119
Lee
Recovery of Citrus germplasm in Florida
$36,970
3
$332,817
$36,970
3
$332,817
$263,788
170
Roberts
Diagnostic Services for growers for detection of HLB to aid in management decisions
$124,740
3
$374,220
$124,740
3
$374,220
$388,528
199
Sieburth
Rutaceous Germplasm Preservation
$71,000
1
$71,000
$71,000
1
$71,000
$459,528
123
Lindeberg
Bioinformatic characterization and development of a central genome resources website for Ca. Liberibacter asiaticus
$140,084
3
$438,641
$140,084
3
$438,641
$599,612
Total for proposals recommended for funding $11,135,206
