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FIGURE 2.4 Gene trees of loci with known function and candidate loci for similar function from Aquilegia. (A) Flavonoid 2-ODDs, including F3H, FLS 1, FLS, and ANS. (B) Flavonoid hydroxylases (F3′H and F3′5′H). (C) Glycosyltransferases (3GT, 5GT, and various others). (D) Myb transcription factors (Mybs affecting the regulation of genes in the flavonoid pathway). Within each tree, the outer lines indicate groups of genes that carry out the same enzymatic function. Aquilegia sequences are indicated by Aq followed by the TC number from the Aquilegia Gene Index or AqMYBcl12 and AqMYBcl17.

FIGURE 2.4 Gene trees of loci with known function and candidate loci for similar function from Aquilegia. (A) Flavonoid 2-ODDs, including F3H, FLS 1, FLS, and ANS. (B) Flavonoid hydroxylases (F3′H and F3′5′H). (C) Glycosyltransferases (3GT, 5GT, and various others). (D) Myb transcription factors (Mybs affecting the regulation of genes in the flavonoid pathway). Within each tree, the outer lines indicate groups of genes that carry out the same enzymatic function. Aquilegia sequences are indicated by Aq followed by the TC number from the Aquilegia Gene Index or AqMYBcl12 and AqMYBcl17.

sequences from Bogs et al. (2006) and those from Nakatsuka et al. (2008b) to identify homologs of UF3GT and UF5GT (Fig. 2.4B and C). Support for the identification of UF3GT and UF5GT homologs in Aquilegia but not one for UF7GT is the fact that Taylor (1984) found only 3- and 5-glucosides and 3,5-diglucoside classes of anthocyanins in Aquilegia.

Using the Aquilegia gene index we identified candidates for nearly all major genes in the flavonoid pathway (Table 2.1 and Fig. 2.4AC). However, 1 key gene, the R2R3-myb transcription factor that controls the expression of multiple genes in the ABP in other systems (Hoballah et al., 2007), appeared to be absent from the gene index. When we searched the index with AN2, the myb transcription factor that controls anthocyanin production in Petunia flowers (Hoballah et al., 2007), the best hit, TC30372,



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