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TABLE 4-3 Near- and Near-to-Intermediate-Term Recommendations

NI-1.

Improve insecticide-based management of ACP.

NI-2.

Support searches for biomarkers that may be exploited to detect CLas-infected citrus.

NI-3.

Establish citrus orchard test plots for evaluation of new scouting and therapeutic methods.

NI-4.

Accelerate the sequencing, assembly, annotation and exploitation of a sweet orange genome to provide a powerful tool for all future citrus improvement research.

NI-5.

Support development of HLB model systems.

NI-6.

Exploit the CLas genome sequence for new strategies of HLB mitigation.

NI-7.

Support research aimed at developing alternative ACP management strategies.

NI-8.

Support small-scale studies on the feasibility of alternative horticultural systems suited to endemic HLB.

NI-9.

Support demonstration of RNA interference (RNAi) effects for possible suppression of ACP.

NI-10.

Develop in vitro culture techniques for CLas to facilitate experimental manipulation of the bacterium for insights into gene function.

NI-11.

Sequence, assemble and annotate the ACP genome to provide a basis for new approaches to ACP management.

Recommendation NI-1. Improve insecticide-based management of ACP.

Approaches recommended here, applied in concert, will support Recommendation O-1, which advocates creation of Citrus Health Management Areas in Florida to facilitate mitigation of HLB and other threats to citrus production. The suppression of ACP populations by insecticide application will be a necessary part of HLB mitigation for the foreseeable future; e.g., until sources of inoculum can be drastically reduced (Recommendations NI-2 and NI-3) or CLas-resistant citrus (see Recommendation L-1) becomes available for planting. We strongly support the idea that present management strategies can be improved by gaining better understanding of ACP and CLas, their ecology and epidemiology, and the presently available (or soon to be available) tools that can aid in their management. Recommendation NI-1 is concerned with improving conventional insecticide suppression of ACP by integrating four types of research and development activities:


Recommendation NI-1a. Develop new methods to fine-tune field surveillance of ACP via more efficient and consistently applied trapping or other methods, to improve timing and targeting of insecticide applications.

At the moment ACP populations are monitored differently in different regions. Additional effort should be invested in developing more effective traps that will provide an accurate assessment of the ACP population so sprays can be more effectively timed. Sampling methods also should be refined within and between areas to help describe actual population trends and help in area-wide management strategies. Monitoring and analyzing ACP populations over the



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