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Appendix
Resources for Studying Aging
INVERTEBRATES
Caenorhabditis Genetics Center fCGC), Division of Biological Sc'-
ences. University of Missouri, Columbia—CGC acquires, stores,
and distributes genetic stocks of Caenorhabditis elegans (a nem-
atode) and relevant bibliographic and genetic information. It re-
ceives nematode strains and mutants and reprints of related pub-
lications and data (raw and analyzed) relevant to nematode ge-
netics; stores these materials; verifies genetic status or scientific
accuracy; distributes bibliographic and genetic information on mu-
tant strains to individual scientists and, through publications, to
the scientific public at large; and distributes mutant strains to
interested scientists. CGC has multi-institute support and the
advantage of multidisciplinary input into its data bases, which
permits it to support a wide range of research interests.
CELL CULTURES
Aging Cell Culture Repository (ACCR), Cornell Institute for Med-
ical Research, Camden, Neu; Jersey- ACCR acquires, develops,
characterizes, stores; ends supplies cell cultures for gerontologic
research. It contains over 600 cell cultures available for research
209
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210
APPENDIX
on aging, including over 200 skin fibroblast cultures from healthy
persons of various ages who are participating in the Baltimore
Longitudinal Study on Aging at the Gerontology Research Center;
skin fibroblast cultures from persons with premature-aging syn-
dromes, including Hutchinson-Gilford syndrome (progeria); and
cultures from clinically documented and at-risk persons, as well as
entire families exhibiting familial Alzheimer's disease. Also avail-
able are human and WI-38 female diploid lung cells available at
early, middle, and late population doubling levels. Cultures of
animal origin include skin fibroblasts from a variety of species of
nonhuman primates, bovine and equine endothelial cells, smooth
muscle and fibroblast cultures, and canine and porcine endothelial
cells.
EXPERIMENTAL POPULATIONS
B'omarker Research Program, National Institute on Aging (NIA)
and; National Center for Toxicological Research (NCTR - NIA, in
conjunction with NCTR, is in the process of developing a colony of
rodents to be used specifically for a biomarker research program.
Those being developed for NIA will be used to develop biomarkers
of aging. Those being raised for NCTR will be used to develop
biomarkers of toxicity, to evaluate the critical assumptions used
in risk assessment. This is a unique program, in that two ma-
jor institutions of the Public Health Service have joined forces in
the use of uniform strains, species, and laboratory conditions to
elucidate sirn~lar end points for different purposes related to their
independent missions. The core of this colony will include four
mouse genotypes (C57BI`/6NNia2, DBA/2NNia2, B6D2FlNia2,
and C3B6F1) and three rat genotypes (F344Nia, BN/BiRijNia,
and the F344BNF1 hybrid), which will be maintained similarly to
the other colonies, but fed either ad libitum or under conditions of
restricted feeding. These colonies wit! be unique, in that ultimately
it will be possible to compare the rodent strains used for geron-
tologic studies with those used for toxicity testing under a range
of conditions. Research availability of the animals will be limited
for gerontologic investigations to those selected by NIA (through
grant or contract mechanisms) alla for toxicologic investigations
to those selected by NCTR for biomarker research.
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APPENDIX
211
RODENTS
Inbred Strains
National Institute on Aging (NIA - Specific-pathogen-free rodents
available or under development from NIA include three rat and
10 mouse genotypes that are raised in barrier facilities and range
in age from 3 to 36 months. Available mouse genotypes are in-
bred strains A/HeNNia, BALB/cNNia, CBA/CaHNNia, C57BL/
6NNia, and DBA/2NNia; hybrids of B6C3FlNia (C57BL/6NNia
X C3H/NNia), B6D2FlNia (C57BL`/66NNia X DBA/2NNia), and
CB6FlNia (BALB/cNNia X C57BL/6NNia); the congenic strain
BALB/cAnNNia-nu~nude); and outbred stock of Swiss Webster.
NIA provides a rat genotype, the inbred Fischer 344 (F344NNia);
however, a colony of three additional genotypes is under develop
meet: the inbred Brown Norway (BN/BiRijNia) and the recipro-
cal FlNia hybrids of the F344 and BN crosses. All rodents are
regularly monitored for genetic purity and health status.
Outbred Strains
National Center for Toxicological Research fNCTR) Specific-
pathogen-free (SPF) rodent resources currently available or
under development include Mus musculus (house mouse) and
Peromyscus leucopus (white-footed mouse). Specimens of both
species were obtained from colonies that were started with founder
stocks trapped in woodiots and old fields at Argonne National
Laboratory in 1962-1964 and bred in the Argonne animal facil-
ity by random ou~crossing. The colonies have been enlarged and
random out-crossing maintained since their transfer to NCTR in
1982. Specimens of M. musculus range in age from weanling to
37 months. Specimens of P. leucopus range in age from weanling
to 84 months. Both species were housed in SPF facilities and fed
laboratory diet NTH-31 and water ad libitum. The animal rooms
are maintained at 70°F ~ 2°F and 40~o ~ No relative humidity
under artificial illumination with a 12:12 light:dark cycle. Cages
are routinely rotated on cage racks to prevent retinal degeneration
from fluorescent lighting. These colonies are small, so animals are
not routinely shipped to other laboratories; however, access to
them for study is available. No charge is made for onsite use.
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212
APPENDIX
NONHUMAN P:R1MATES
National Institute on Aging (NIA} NIA maintains approximately
300 nonhuman primates (mainly Macaca mulatto, with a few M.
nemestrina) at five regional primate centers for conducting re-
search on aging. The animals are approximately 18-35 years old.
About two-thirds are available for noninvasive research, and the
remaining one-third for invasive research.
Representative terms from entire chapter:
fibroblast cultures
