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Appendix Resources for Studying Aging INVERTEBRATES Caenorhabditis Genetics Center fCGC), Division of Biological Sc'- ences. University of Missouri, ColumbiaCGC acquires, stores, and distributes genetic stocks of Caenorhabditis elegans (a nem- atode) and relevant bibliographic and genetic information. It re- ceives nematode strains and mutants and reprints of related pub- lications and data (raw and analyzed) relevant to nematode ge- netics; stores these materials; verifies genetic status or scientific accuracy; distributes bibliographic and genetic information on mu- tant strains to individual scientists and, through publications, to the scientific public at large; and distributes mutant strains to interested scientists. CGC has multi-institute support and the advantage of multidisciplinary input into its data bases, which permits it to support a wide range of research interests. CELL CULTURES Aging Cell Culture Repository (ACCR), Cornell Institute for Med- ical Research, Camden, Neu; Jersey- ACCR acquires, develops, characterizes, stores; ends supplies cell cultures for gerontologic research. It contains over 600 cell cultures available for research 209

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210 APPENDIX on aging, including over 200 skin fibroblast cultures from healthy persons of various ages who are participating in the Baltimore Longitudinal Study on Aging at the Gerontology Research Center; skin fibroblast cultures from persons with premature-aging syn- dromes, including Hutchinson-Gilford syndrome (progeria); and cultures from clinically documented and at-risk persons, as well as entire families exhibiting familial Alzheimer's disease. Also avail- able are human and WI-38 female diploid lung cells available at early, middle, and late population doubling levels. Cultures of animal origin include skin fibroblasts from a variety of species of nonhuman primates, bovine and equine endothelial cells, smooth muscle and fibroblast cultures, and canine and porcine endothelial cells. EXPERIMENTAL POPULATIONS B'omarker Research Program, National Institute on Aging (NIA) and; National Center for Toxicological Research (NCTR - NIA, in conjunction with NCTR, is in the process of developing a colony of rodents to be used specifically for a biomarker research program. Those being developed for NIA will be used to develop biomarkers of aging. Those being raised for NCTR will be used to develop biomarkers of toxicity, to evaluate the critical assumptions used in risk assessment. This is a unique program, in that two ma- jor institutions of the Public Health Service have joined forces in the use of uniform strains, species, and laboratory conditions to elucidate sirn~lar end points for different purposes related to their independent missions. The core of this colony will include four mouse genotypes (C57BI`/6NNia2, DBA/2NNia2, B6D2FlNia2, and C3B6F1) and three rat genotypes (F344Nia, BN/BiRijNia, and the F344BNF1 hybrid), which will be maintained similarly to the other colonies, but fed either ad libitum or under conditions of restricted feeding. These colonies wit! be unique, in that ultimately it will be possible to compare the rodent strains used for geron- tologic studies with those used for toxicity testing under a range of conditions. Research availability of the animals will be limited for gerontologic investigations to those selected by NIA (through grant or contract mechanisms) alla for toxicologic investigations to those selected by NCTR for biomarker research.

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APPENDIX 211 RODENTS Inbred Strains National Institute on Aging (NIA - Specific-pathogen-free rodents available or under development from NIA include three rat and 10 mouse genotypes that are raised in barrier facilities and range in age from 3 to 36 months. Available mouse genotypes are in- bred strains A/HeNNia, BALB/cNNia, CBA/CaHNNia, C57BL/ 6NNia, and DBA/2NNia; hybrids of B6C3FlNia (C57BL/6NNia X C3H/NNia), B6D2FlNia (C57BL`/66NNia X DBA/2NNia), and CB6FlNia (BALB/cNNia X C57BL/6NNia); the congenic strain BALB/cAnNNia-nu~nude); and outbred stock of Swiss Webster. NIA provides a rat genotype, the inbred Fischer 344 (F344NNia); however, a colony of three additional genotypes is under develop meet: the inbred Brown Norway (BN/BiRijNia) and the recipro- cal FlNia hybrids of the F344 and BN crosses. All rodents are regularly monitored for genetic purity and health status. Outbred Strains National Center for Toxicological Research fNCTR) Specific- pathogen-free (SPF) rodent resources currently available or under development include Mus musculus (house mouse) and Peromyscus leucopus (white-footed mouse). Specimens of both species were obtained from colonies that were started with founder stocks trapped in woodiots and old fields at Argonne National Laboratory in 1962-1964 and bred in the Argonne animal facil- ity by random ou~crossing. The colonies have been enlarged and random out-crossing maintained since their transfer to NCTR in 1982. Specimens of M. musculus range in age from weanling to 37 months. Specimens of P. leucopus range in age from weanling to 84 months. Both species were housed in SPF facilities and fed laboratory diet NTH-31 and water ad libitum. The animal rooms are maintained at 70F ~ 2F and 40~o ~ No relative humidity under artificial illumination with a 12:12 light:dark cycle. Cages are routinely rotated on cage racks to prevent retinal degeneration from fluorescent lighting. These colonies are small, so animals are not routinely shipped to other laboratories; however, access to them for study is available. No charge is made for onsite use.

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212 APPENDIX NONHUMAN P:R1MATES National Institute on Aging (NIA} NIA maintains approximately 300 nonhuman primates (mainly Macaca mulatto, with a few M. nemestrina) at five regional primate centers for conducting re- search on aging. The animals are approximately 18-35 years old. About two-thirds are available for noninvasive research, and the remaining one-third for invasive research.