1) Encourage early practitioners to document and publish protocols as individual methods are developed.

2) Begin collaborative development of new methods and protocols only after preliminary video protocols and sufficient well-characterized nanomaterials are available to support method development and testing.

3) Use a material registry system to designate unique lot descriptors for each nanomaterial sample, to maintain a catalog of descriptors to capture lot-to-lot variability in engineered nanomaterials (ENMs), and to correlate possibly different effects seen in various uses and analyses of material lots. Monitor shipping and record environmental conditions during transit of the nanomaterial and any biologic materials needed for use in interlaboratory studies (ILSs), including calibration efforts.

4) Accelerate progress in developing standard analytic methods by using video (particularly common video equipment and applications, such as cellular telephones) and digital collaboration environments (for example, wikis, RSS feeds, and Facebook) to facilitate broad participation and communication.

5) Conduct informal interlaboratory testing of the protocols to identify causes of laboratory bias and to investigate the ruggedness and reliability of the methods before development of the documentary standard. It may be faster to achieve consensus on the documentary standard because of the prior vetting of the protocol and the existence of the informal ILS results, robustness data, and video (JoVE 2011).

6) Rapidly modify the video protocol through a small ILS testing group so that consensus is reached and the (informal) error and uncertainty of the method are satisfactory.

7) Collect data to substantiate that the ruggedness and robustness of the method are adequate (quantification of the sensitivity of the method to variation in any experimental procedure, materials, or conditions is archived with the video protocol).

8) Develop a consensus documentary standard based on the video and the results of the informal ILSs and a more polished video illustrating the method.

9) Use the documentary standard and supplementary video in formal ILSs to determine the error and uncertainty of the method.

10) Publish

a) The documentary consensus standard.

b) The final video (to be used for training).

c) The error and uncertainty data and analysis.

d) The sensitivity data and analysis.

11) Establish a reporting standard for the level of validation achieved by a laboratory that is using the method (for example, full validation, partial validation, corroboration with at least one other laboratory, or a single laboratory result).

12) Continue to engage the stakeholder group through the collaborative environment to

The National Academies | 500 Fifth St. N.W. | Washington, D.C. 20001
Copyright © National Academy of Sciences. All rights reserved.
Terms of Use and Privacy Statement