percent of the duck population. Dose levels of Margosan-O® ranged at 1-16 ml/kg of body weight. Observations showed no negative effects and all ducks remained active and healthy throughout the 14-day experimental period. The acute LD50 of Margosan-O® to mallard ducks is in excess of 16.0 ml/kg.

Test 2—

Avian Dietary LC50 (lethal concentration) with bobwhite quail. The birds were given their basal diet, with additions of Margosan-O® ranging from 1,000 to 7,000 ppm. Observations showed no negative effects and the quail were active and healthy throughout the 5-day test period and 3-day recovery phase. The acute oral LC50 of the Margosan-O® to bobwhite quail is therefore in excess of 7,000 ppm.

Test 3—

Avian Dietary LC50 Study with mallard ducks. The ducks were given a basal diet, plus Margosan-O® ranging from 1,000 to 7,000 ppm concentrate for 5 days. No mortalities resulted. The ducks were active and healthy throughout the test and recovery phases. The acute LC50 of the test material to mallard ducks is therefore in excess of 7,000 ppm.

Test 4—

(No. 1), Acute Toxicity of Margosan-O® to rainbow trout. This test involved a 96-hour LC50 of Margosan-O® at various concentrations. The LC50 was 8.8 ml of Margosan-O® per liter of water, and the 96-hour no-observed-effect concentration was 5 mg/l.

Test 4—

(No. 2), Acute Toxicity of Margosan-O® to bluegill sunfish. The results showed a 96-hour LC50 of 37 mg/l and a no-observed-effect 96-hour level of 20 mg/l. In the fish bioassay tests with trout and sunfish there were behavioral responses in static water that could probably not occur in moving water.

Test 5—

Acute Toxicity of Margosan-O® to Daphnia magna, the water flea. The test was done on newly molted in stars less than 20 hours old which were placed in a fresh aquatic habitat for up to 48 hours. The LC 50 was 13 mg/l and the no-observed-effect concentration at 48 hours was less than 10 mg/l. Therefore, the toxicity value obtained was well within the expected range, but it indicates that Margosan-O® will affect primitive aquatic invertebrates under static conditions.

Test 6—

Acute Oral Toxicity. Rats were dosed once and then observed for 14 days for abnormal behavior or mortality. No negative effects were observed and the acute oral toxicity of the test material was in excess of 5 ml/kg, the limit of the required test.

Test 7—

(No. 1), Acute Dermal Toxicity. A nonpermeable patch containing 2 ml/kg body weight of Margosan-O® was placed over small shaved areas on a group of albino rabbits. No



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