This suggests that the changes noted above were not treatment-related, but rather that they were normal and not unusual. The results of this study suggest that the test material does not cause an adverse immune response.

Test 11—

Sensitization. This test was done on guinea pigs, which were shaved and patched with Margosan-O® test material for 6 hours. The procedure was repeated on alternate days for a total of nine applications. A retest dose was applied after 14 days with duplicate patches, and the reaction was read 24 hours later. Margosan-O® does not produce sensitization.

Test 12—

Mutagenicity. This is the traditional Ames mutagenicity study used in the United States on five strains of Salmonella typhimurium. Results of this test indicate that the MargosanO® concentrate is nonmutagenic.

Test 13—

Bee Adult Toxicity Test. This test was done voluntarily and was not ordered by the EPA. With the assistance of the University of California's Apiary at Riverside, California, Vikwood, Ltd. ordered a "Bee Adult Toxicity Test" on honeybee worker adults. Margosan-O® was administered as a direct contact chemical using field dosages up to 4,478 ppm A.I./ha. It was found to be benign to honeybees at well above the recommended dosage of 20 ppm (diluted, as a foliar spray) for a common pest, the gypsy moth, Lymantria dispar (L.).



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