(by W. D. McElroy) as assistant professors at the McCollum-Pratt Institute of the Department of Biology of Johns Hopkins University. At Hopkins, Nate and Sidney developed a successful and productive collaboration studying the chemistry of the pyridine nucleotide coenzymes and the enzymes that are involved with them. This collaboration led to the founding in 1955 of the classic series, Colowick and Kaplan's Methods in Enzymology, which now has more than 140 volumes with more in press.
In 1957 Nate left Johns Hopkins University to become founding chairman of the Graduate Department of Biochemistry at Brandeis University. To establish the new department he, in association with Martin Kamen who joined him at Brandeis, hired about a dozen carefully selected young assistant professors and brought them to a campus where very little space was available for them for at least a year. Under these conditions and with Nate as catalyst, an uncommon camaraderie developed between faculty, postdoctoral fellows, graduate students, and staff that led to scientific productivity of such caliber that his fledgling department gained international recognition in a very short time. By recognition for his department, Nate Kaplan played a major role in establishing Brandeis, which had only been founded in 1948, as a major, research oriented university in the sciences in the 1960s. His research at Brandeis was primarily concerned with the structure-function relationships of dehydrogenases, which led him into the areas of enzyme evolution and isoenzymes. He was one of the first to recognize the potential of using isoenzymes analysis in clinical diagnosis and for this reason developed methods for detecting lactate dehydrogenase isoenzymes in human serum.
In 1968, pulled by the urgings of Martin Kamen who