unnecessary traffic (AORN 1982; Bartley 1993). In some circumstances, it might be necessary to use an operating room for other purposes. In such cases, it is imperative that the room be returned to an appropriate level of cleanliness before its use for major survival surgery.

Careful surgical monitoring and timely attention to problems increase the likelihood of a successful surgical outcome. Monitoring includes checking of anesthetic depth and physiologic function and assessment of clinical signs and conditions. Maintenance of normal body temperature minimizes cardiovascular and respiratory disturbances caused by anesthetic agents (Dardai and Heavner 1987) and is of particular importance.

The species of animal influences the components and intensity of the surgical program. The relative susceptibility of rodents to surgical infection has been debated; available data suggest that subclinical infections can cause adverse physiologic and behavioral responses (Beamer 1972; Bradfield and others 1992; Cunliffe-Beamer 1990; Waynforth 1980, 1987) that can affect both surgical success and research results. Some characteristics of common laboratory-rodent surgery-such as smaller incision sites, fewer personnel in the surgical team, manipulation of multiple animals at one sitting, and briefer procedures-as opposed to surgery in larger species, can make modifications in standard aseptic techniques necessary or desirable (Brown 1994; Cunliffe-Beamer 1993). Useful suggestions for dealing with some of the unique challenges of rodent surgery have been published (Cunliffe-Beamer 1983, 1993).

Generally, farm animals maintained for biomedical research should undergo surgery with procedures and in facilities compatible with the guidelines set forth in this section. However, some minor and emergency procedures that are commonly performed in clinical veterinary practice and in commercial agricultural settings may be conducted under less-stringent conditions than experimental surgical procedures in a biomedical-research setting. Even when conducted in an agricultural setting, these procedures require the use of appropriate aseptic technique, sedatives, analgesics, anesthetics, and conditions commensurate with the risk to the animal's health and well-being. But they might not require the intensive surgical settings, facilities, and procedures outlined here.

Presurgical planning should specify the requirements of postsurgical monitoring, care, and record-keeping, including the personnel who will perform these duties. The investigator and veterinarian share responsibility for ensuring that postsurgical care is appropriate. An important component of postsurgical care is observation of the animal and intervention as required during recovery from anesthesia and surgery. The intensity of monitoring necessary will vary with the species and the procedure and might be greater during the immediate anesthetic-recovery period than later in postoperative recovery. During the anesthetic-recovery period, the animal should be in a clean, dry area where it can be observed often by trained personnel. Particular attention should be given to thermoregulation, cardiovascular and respiratory function, and postoperative pain or discom-



The National Academies | 500 Fifth St. N.W. | Washington, D.C. 20001
Copyright © National Academy of Sciences. All rights reserved.
Terms of Use and Privacy Statement