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TABLE A9-1 Composition of Representative Diets for Rats and Chicksa

Ingredient

Rat Diet (g/kg)

Chick Diet (g/kg)

Milk powder

 

250

Casein, high protein

200

150

Ground corn, acid washed

673

230

Non-nutritive fiber

 

50

Dextrose, anhydrous

 

142.5

Corn oil

 

100

Soybean oil

70

 

CaCo3

15

14

KH2PO4

10

4

Choline bitartrate

2.5

 

A vitamin mix in cornstarch and a mineral mix in dextrose was added to the rat diet to furnish all known miconutrient requirements. The chick diet was slightly different to meet requirements of that species.

Vitamins added to diet: (mg/kg): Niacin (30); d-pantothenic acid calcium (16); pyridoxin HCI (7); thiamin HCI (6); riboflavin (6); folic acid (2); d-biotin (0.2); vitamin B12(0.02); d,l-a-tocopherol acetate (120); retinyl palmitate (16); vitamin D3 (2.5); vitamin K, (0.75).

Trace elements added to diet: (mg/kg): CaHPO4 (1,265); K2SO4 (1,632); CaCI2.2H2O (1,000); Na2CO3 (1,500); Mg(C2H3O2)2.4H2O (3,600); NaSiO3.9H2O (254); ferric citrate (220); zinc carbonate (58); manganese carbonate (22); copper acetate (11); KIO3 (0.35); Na2SeO4 (0.36 mg/kg); NH4MO7O24.4H2O (0.37); CrK(SO4)2.12 H2O (9.6); H3BO3 (2.9); NaF (2.2); NiCO3 (2.2); SnO (0.25); NH4VO3 (0.46).

aData from Nielsen et al. 1978; Nielsen 1980; Uthus et al. 1983; Uthus 1992.

Goats and Minipigs

The animals are kept in plastic-lined wooden cages with cellulose as bedding material. An estimated 100 g per day of cellulose (arsenic at less than 15 ng/g) is eaten by goats. Cellulose contributes to their energy intake and dilutes the arsenic content from less than 35 ng/g in the semisynthetic diet to less than 20 ng/g in the total. Table A9-2 gives the composition of the rations fed ad libitum to both species.



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