. "Summary of Advantages and Disadvantages of In Vitro and In Vivo Methods." Monoclonal Antibody Production. Washington, DC: The National Academies Press, 1999.
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Advantages of Mouse Ascites Method
The mouse ascites method usually produces very high mAb concentrations that often do not require further concentration procedures that can denature antibody and decrease effectiveness.
The high concentration of the desired mAb in mouse ascites fluid avoids the effects of contaminants in in vitro batch-culture fluid when comparable quantities of mAb are used.
The mouse ascites method avoids the need to teach the antibody producer tissue-culture methods.
Disadvantages of Mouse Ascites Method
The mouse ascites method involves the continued use of mice requiring daily observation.
MAb produced by in vivo methods can contain various mouse proteins and other contaminants that might require purification.
The mouse ascites method can be expensive if immunodeficient mice in a barrier facility must be used.
In vivo methods can cause significant pain or distress in mice.
