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110 PART II. BIOSYNTHESIS OF HEMOGLOBIN on copper metabolism. XVIII. Skeletal changes associated with copper deficiency in swine, 97: 405, 1955. 7. Bush, J. A.. Jensen, W. N., Athens, J. W., Ashenbrucl~er, H., Cartwright, G. E., and Wintrobe, M. M.: Studies on copper metabolism. NIX. The kinetics of iron metabolism and erythrocyte life-span in copper-deficient swine, J. Exp. Med., 103: 701, 1956. 8. Gubler, C. J., Cartwright, G. E., and NVintrobe, M. M.: Studies on copper metab- olism. XX. Enzyme activities and iron metabolism in copper and iron deficiencies, J. Biol. Chem., 224: 533, 1957. 9. Elvehjem, C. A.: The biological significance of copper and its relation to iron metabolism, Physiol. Rev., 15: 471, 1935. 10. Schultze, M. O.: Metallic elements and blood formation, Physiol. Rev.'20: 37, 1940. 11. Marston, H. R.: Cobalt, copper and molybdenum in the nutrition of animals and plants, Physiol. Rev., 32: 66, 1952. 12. Markowitz, H., Cartwright, G. E., and Wintrobe, M. M.: Unpublished obser- vations. 13. Mann, T., and Keilin, D.: Haemocuprein and hepatocuprein; copper-protein com- pounds of blood and liver in mammals, Proc. Royal Soc., London, Series B. 126: 303, 1938. 14. Holmberg, C. G., and Laurell, C. B.: Investigations in serum copper. II. Isolation of the copper containing protein, and a description of some of its properties, Acta Chem. Scandinav., 2: 550, 1948. DISCUSSION Dr. I. H. Scheinberg: I was curious, Dr. Cartwright, as to the difference between erythrocuprein and the hemocuprein that was reported about 20 years ago which, I think, had the same physical characteristics. Dr. Edsall: It certainly had a very similar molecular weight. Dr. Cartwright: The molecular weight and copper concentration of ery- throcuprein are the same as for hemocuprein, the erythrocyte copper protein isolated by Mann and Keilin from ox blood. We have chosen to call our compound erythrocuprein for the following reasons. Hemocuprein from ox blood is blue and erythrocuprein is colorless; the isolation procedure used by us was quite different from the procedure used by Mann and Keilin; their protein was isolated from ox blood and not from human blood. Finally, Mann and Keilin used the term hemocuprein for both the serum copper protein and the red cell copper protein. The serum copper protein has since been isolated by Holmberg and Laurell, as well as by others. It is a distinctly different protein from the erythrocuprein which we have described. Therefore, two blood copper proteins (hemocupreins) have now been isolated, namely, cerulo- plasmin and erythrocuprein. Thus, it would seem advisable to use the term hemocupreins to refer to all blood copper proteins collectively.