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A METHOD FOR THE CHARACTERIZATION OF ABNORMAL HUMAN HEMOGLOBINS BASED UPON DIFFERENCES IN CHROMATOGRAPHIC BEHAVIOR ON AMBERLITE IRC 50* T. H. J. HUISMAN Next to the electrophoretic techniques for the identification of abnormal hemoglobins, the new chromatographic method using the cation exchanger Amberlite IRC 50 may be most valuable. This simple routine method, which was described in detail in 1955,1 is found to be useful not only for the identi- fication of the hemoglobins A, S. C, E and IF 2 but also for some rare types of human hemoglobin. In figure 1 the chromatographic behavior of nine dif- ferent types of human hemoglobin (A, S. C, D, E, F. H. I and J) is given. of.. ~ ~ . :::: :::::: FIG. 1. The separation of different abnormal human hemoglobins on Amberlite IRC-50. Citrate builder pH 6.0, sodium ion concentration 0.15. 1. C + S ~ A + F 4. A + H 2. D + A 5. A + I 3. E + A + Fit 6. A + J (unseparated) Next to the well known separation of the hemoglobins C, S. A and Fit it was found: a. Fib D has the same mobility as sickle-cell hemoglobin. b. Especially when a small amount of hemoglobin ~ about 5 ma. ~ chromatographed, a complete separation of a mixture of the hemo- globins A, E and Fit into the three components is possible. c. Hb H shows a high mobility and is easily distinguished from fetal hemoglobin. ~ Dr. Huisman was unable to attend the Conference and this paper was read and the illustrations shown by Dr. London. 165 . IS