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Toxicity of Military Smokes and Obscurants: Volume 3 Appendix B Vat Yellow 4 BACKGROUND AN ALTERNATIVE name for vat yellow 4 is dibenzochrysenedione (DBC). Vat yellow 4 is a component of the old yellow-and green-dye mixtures. TOXICOKINETICS No studies have been conducted on the toxicokinetics of vat yellow 4. TOXICITY SUMMARY Effects in Humans There have been no reports of humans exposed either accidentally or in controlled laboratory environments to vat yellow 4. Effects in Animals ONE-TIME EXPOSURE A commercial report (Charm 1976, as cited in Dacre et al. 1979) describing acute oral and dermal toxicity tests with a vat yellow 4 paste formu-
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Toxicity of Military Smokes and Obscurants: Volume 3 lation (unspecified concentration) indicated low acute oral and dermal toxicity in rats and rabbits. The oral lethal dose for 50% of the test animals (LD50) for this paste in rats was greater than 46 grams per kilogram (g/kg) of body weight. The minimal oral LD50 in rabbits was 11.6 g/kg of body weight. Application of the paste to intact and abraded skin of rabbits produced no grossly discernible skin damage, and the acute dermal LD50 for rabbits was greater than 4.6 g/kg of body weight. Instillation of the paste into the eyes of rabbits produced minimal reversible ocular irritation. CARCINOGENICITYAND MUTAGENICITY Subcutaneous injection or painting of the skin of mice with vat yellow 4 for prolonged periods did not produce tumors (Kleinenberg 1939, as cited in Dacre et al. 1979). Another report of similar studies in mice indicated high mortality but no tumors in the mice (Shubik and Hartwell 1957, as cited in Dacre et al. 1979). Concentrations of vat yellow 4 used in the study were not reported by Dacre et al. (1979). Epler (1979) reported that vat yellow 4 was positive in the Ames mutagenicity assay. However, Zeiger et al. (1987) and Sigman et al. (1985) report vat yellow 4 to be negative in the same assay. Harrington-Brock et al. (1991) report vat yellow 4 to be positive only with activation in the thymidine kinase locus and micronuclei assays in mouse lymphoma cells. A standard cancer bioassay was conducted by the National Cancer Institute (NCI 1979) on a commercial product reported by the manufacturer to contain 18.2% vat yellow 4, 30.8% sorbitol, 5.5% dispersant (Lomar TWC), 2.7% glycerin, and 42.8% water. The test was negative for male and female rats administered vat yellow 4 at 3,500 or 7,000 parts per million (ppm) in the diet and female mice administered 12,500 or 25,000 ppm in diet but caused an increased incidence of lymphomas in male mice fed 50,000 ppm. Because the study was done on the mixture, the contribution of vat yellow 4 to the carcinogenicity is uncertain (Ashby and Tennant 1988). SUBCOMMITTEE EVALUATION OF DYE TOXICITY The carcinogenic potential of vat yellow 4 is uncertain, and animal studies indicate a low toxicity via oral or dermal routes of exposure. The
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Toxicity of Military Smokes and Obscurants: Volume 3 toxicity of inhaled vat yellow 4 is unknown and should be investigated if the compound is to be used in colored smokes. REFERENCES Ashby, J., and R.W. Tennant. 1988. Chemical structure, Salmonella mutagenicity and extent of carcinogenicity as indicators of genotoxic carcinogenesis among 222 chemicals tested in rodents by the U.S. NCI/NTP. Mutat Res. 204(1):17-115. Charm, J.B. 1976. Letter from J.B. Charm, Allied Chemical Corp., Morristown, NJ, to Cpt. R.N. Shiotsuka, USAMBRDL. Nov. 8. Dacre, J.C., W.D. Burrows, C.W.R. Wade, A.F. Hegyeli, T.A. Miller, D.R. Cogley. 1979. Problem Definition Studies on Potential Environmental Pollutants. V. Physical, Chemical, Toxicological, and Biological Properties of Seven Chemicals Used in Pyrotechnic Compositions. Tech. Rep. No. 7704. AD A090631. U.S. Army Medical Bioengineering Research and Development Laboratory, Fort Detrick, Frederick, MD. Epler, J.L. 1979. U.S. Army Project Order No. 9600. Oak Ridge National Laboratory, Oak Ridge, TN. Harrington-Brock, K., L. Parker, C. Doerr, M.C. Cimino, and M.M. Moore . 1991. Analysis of the genotoxicity of anthraquinone dyes in the mouse lymphoma assay. Mutagenesis 6(1):35-46. Kleinenberg, G.E. 1939. The blastogenic effect of 3,4,8,9-dibenzopyrene and some of its derivatives. III. Studies on the 5,10-quinone of 3,4,8,9-dibenzopyrene and its indigosol. Arch. Sci. Biol. (Leningrad) 56(3):48-52. NCI (National Cancer Institute). 1979. Bioassay of C. I. Vat Yellow 4 for Possible Carcinogenicity. Carcinogenesis Tech. Rep. 134. DHEW Publ. No. (NIH) 79-1389. National Institutes of Health, National Cancer Institute, Bethesda, MD. Shubik, P., and J.L. Hartwell, 1957. Survey of Compounds Which Have Been Tested for Carcinogenic Activity. Public Health Service Publ. 149, 2nd Ed., Suppl. I. Washington, D.C.: U.S. Government Printing Office. Sigman, C.C., P.A. Papa, M.K. Doeltz, L.R. Perry, A.M. Twhigg, and C.T. Helmes. 1985. A study of anthraquinone dyes for the selection of candidates for carcinogen bioassay. J. Environ. Sci. Health A20(4):427-484. Zeiger, E., B. Anderson, S. Haworth, T. Lawlor, K. Mortelmans, and W. Speck. 1987. Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals. Environ. Mutagen 9(Suppl 9):1-109.
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