Cover Image

PAPERBACK
$19.95



View/Hide Left Panel
"http://www.w3.org/TR/2000/REC-xhtml1-20000126/DTD/xhtml1-transitional.dtd">

TABLE 5. Amino acid and nucleotide sequence of the RATs and their incidence

 

Motif

falciparum

reichenowi

RAT

Amino acid

Nucleotide

%

Number

%

Number

A

NANP

aatgcaaaccca

55.1

566

38.5

10

B

NANP

. . . . . . . . t . . t

16.1

165

30.8

8

C

NANP

. . . . . . . . t . . .

7.6

78

 

D

NANP

. . . . . c . . t . . a

6.2

64

3.8

1

E

NANP

. . . . . . . . . . . c

6.2

64

F

NANP

. . c . . . . . . . . c

5.1

52

G

NANP

. . . . . c . . . . . .

3.1

32

7.7

2

H

NANP

. . . . . . . . . . . t

0.3

3

I

NANP

. . c . . . . . . . . .

0.2

2

3.8

1

J

NANP

. . . . . . c . . . . c

0.1

1

Z

NANP

. . . . . . . . t . . c

15.4

4

M

NVDP

. . . . t . g . t . . .

52.3

46

20.0

1

N

NVDP

. . . . t . g . t . . c

31.8

28

40.0

2

O

NVDP

. . c . t . g . t . . t

14.8

13

P

NVDP

. . . . t . g . t . . t

1.1

1

20.0

2

X

NVNP

. . . . t . . . t . . c

100.0

4

Modified from Rich et al. (1997); see Ayala et al. (1999).

same as the falciparum average) and three rather than only two amino acid motifs, two of them identical to those of falciparum.

Nearly all of the synonymous site differences observed in the CR are between RATs that exist within any single allele. This is a strong indication that while RAT diversity may have an ancient origin, it has been maintained within individual alleles and therefore can withstand even the most constricted bottleneck. For example, all 25 Csp CR alleles contain at least one copy of each of the most common RATs (A, B, C, D, E, and F, which amount to 96% of all NANP repeats; and M and N, which amount to 84% of all NVDP repeats). If any one of these alleles were the sole survivor following a bottleneck, it alone would possess nearly all of the

TABLE 6. Number of RATs in the Csp gene sequences of P. falciparum and in P. reichenowi

 

Number of sequences

Different RATs per sequence

Total RATs per sequence

   

9

10

11

35

40

41

42

43

44

46

47

49

51

P. falciparum

25

12

8

5

 

1

2

2

2

6

8

1

2

1

P. reichenowi

1

 

1

1



The National Academies | 500 Fifth St. N.W. | Washington, D.C. 20001
Copyright © National Academy of Sciences. All rights reserved.
Terms of Use and Privacy Statement