National Academies Press: OpenBook

Biosafety in the Laboratory: Prudent Practices for Handling and Disposal of Infectious Materials (1989)

Chapter: Appendix F: Teaching Aids and Training Courses

« Previous: Appendix E: Regulations Governing the Packaging, Labeling, and Transport of Infectious Agents
Suggested Citation:"Appendix F: Teaching Aids and Training Courses." National Research Council. 1989. Biosafety in the Laboratory: Prudent Practices for Handling and Disposal of Infectious Materials. Washington, DC: The National Academies Press. doi: 10.17226/1197.
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Page 205
Suggested Citation:"Appendix F: Teaching Aids and Training Courses." National Research Council. 1989. Biosafety in the Laboratory: Prudent Practices for Handling and Disposal of Infectious Materials. Washington, DC: The National Academies Press. doi: 10.17226/1197.
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Page 206
Suggested Citation:"Appendix F: Teaching Aids and Training Courses." National Research Council. 1989. Biosafety in the Laboratory: Prudent Practices for Handling and Disposal of Infectious Materials. Washington, DC: The National Academies Press. doi: 10.17226/1197.
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Page 207

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Appendix F Teaching Aids and Training Courses SOURCES OF TEACHING AIDS 1. State Health Laboratory Directors (loan, no charged a. Safety Management in the Laboratory CDC-76-24, John Forney, Ph.D. (35 slides and 22-page handout) Describes safety regulations and requirements under OSHA and CAP; hazards frequently seen; and re- sponsibilities placed upon management, supervisors, and employees. Develops elements of a safety pro- gram. b. Controlling Infectious Aerosols: Part I- Precautions in Microbiology, produced by CDC, 1976; on loan for return postage only, 16-mm firm or videocassette, color and sound. Demonstrates common hazards in the microbiology laboratory and shows how they produce infectious aerosols. Discusses ways of becoming infected in the laboratory and the factors determining infection. Controlling Infectious Aerosols: Part II- Minimizing Equipment-Related Hazards, produced by the CDC, 1976; on loan for return postage, 16-mm film or videocas- sette, color and sound. 205 Discusses how to use and maintain equipment in a microbiological laboratory. Demonstrates proper use of aerosol-free blender and centrifuge in preventing contamination. Demonstrates use of negative pres- sure (Class I) and laminar flow (Class II) biological safety cabinets in reducing exposure to infectious agents. 2. National Institutes of Health (on loan) Division of Safety Bethesda, MD 20892 a. Assessment of Risk in the Cancer Virus Laboratory b. Certification of Class II ([=ninar Flow) Biological Safety Cabinets d. Effective Use of the Laminar Flow Biological Safety Cabinets Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets Fundamentals for Safe Microbiological Research f. Hazard Control in the Animal Laboratory g. Selecting a Biological Safety Cabinet

206 APPENDIX F 3. National Safety Council 444 N. Michigan Avenue Chicago,IL 60611 Introduction to Biohazards Control Stock No. 176.54 Slide-tape cassette 4. National Audiovisual Center National Archives and Records Administration Customer Services Section CL 8700 Edgeworth Drive Capitol Heights, MD 20743-3701 Telephone number: 1-800-638-1300 (credit card orders) a. Fundamentals for Safe Microbiological Research A Series 5 slide sets with instructor manuals 1 vi~assette, with study guide, 1983 Division of Safety, National Institutes of Health Series No. A09753/CL i. Host Parasite Relationships (Unit 1) Describes the role of pH, temperature, and aerobic and anaerobic environment in micro- bial growth and metabolism; mechanisms of cell growth division; and the replication ~ . processes for viruses. 81 color slides, silent, rampage instructor manual Title No. A097 54/CL ii. Microbial Ecology (Unit 2) Covers the chemical and physical proper- ties of the natural habitats that influence the survival and replication of an organism, as well as why an organism is unable to endure and proliferate in natural ecosystems. 37 color slides, silent, 56-page instructor manual Title No. A09755/CL iii. Principles of Physical and Chemical Containment (IJnit 3) Defines contamination, explains the prin- ciples of contamination control, and lists three types of contamination problems. 257 color slides, silent, 280-page instructor manual Title No. A09756/CL i' v. Biological Containment for Recombi- nant DNA Molecules (Unit 4) Levels of biological containment, catego- rizing experiments, and He roles played by investigators and NIH committees. Recom- binant DNA techniques as defined by the guidelines. 6 color slides, silent, 84-page instructor manual Title No. A09757/CL v. Laboratory Skills (IJnit 5) Aseptic technique; principles and purposes of isolating bacteria from mixed broth cul- tures onto agar plates, staining techniques, and making dilutions and pour plates from liquid suspensions; and the mles for count- ing colonies after incubation of pour plates. 123 color slides, silent, 92-page instructor manual Title No. A09758/CL b. Using the Gravity Displacement Steam Autoclave In the Biomedical Laboratory Demonstrates aseptic and safe techniques and procedures for preparing, processing, and handling materials undergoing steam sterilization and decontamination. 29-minute videocassette, study guide 3/4-inch video no. A10296/CL VHS no. A1037VCL Beta 2 no. A10371/CL

APPENDIX F TRAINING COURSES 1. Control of Biohazards in the Research Labora- tory: A training course for biosafety officers. A one-week course offered annually (third week in July) consisting of lectures and laboratory exer- cises. Lectures include an overview of cell biology and biotechnology; hazard potential of infectious agents, recombinant DNA, and oncogenic viruses; dissemination of contaminants; equipment designed for safety; containment concepts: primary and secon- da~y barriers; laboratory design criteria; personal practices; safe handling and housing of laboratory animals; principles of ventilation; radiation safety; decontamination, disinfection, and sterilization; how to plan, organize, develop, and conduct a laboratory safety program; emergency procedures; and federal regulations involving laboratory safety. 207 For further information contact: Course Co- Direc~r, Office of Safety and Environmental Health, The Johns Hopkins Institutions, 2021 E. Monument Street, Baltimore, MD 21205. Telephone number 301-955-5918. 2. Biological Safety Cabinet Certification Workshop A one-week course offered annually to anyone responsible for certifying or coordinating the certifi- cation of biological safety cabinets. The course con- sists of lectures and laboratories covering the con- struction, operation, decontamination, and testing of biological safety cabinets. For further information, contact Dr. Melvin First, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115. Telephone number 617-732-1 168.

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Biosafety in the Laboratory is a concise set of practical guidelines for handling and disposing of biohazardous material. The consensus of top experts in laboratory safety, this volume provides the information needed for immediate improvement of safety practices. It discusses high- and low-risk biological agents (including the highest-risk materials handled in labs today), presents the "seven basic rules of biosafety," addresses special issues such as the shipping of dangerous materials, covers waste disposal in detail, offers a checklist for administering laboratory safety—and more.

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