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Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
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4
References

Abramovitch, R. B., J. C. Anderson, et al. (2006). “Bacterial elicitation and evasion of plant innate immunity.” Nat Rev Mol Cell Biol 7(8): 601-611.

Afonnikov, D. A. and N. A. Kolchanov (2004). “CRASP: a program for analysis of coordinated substitutions in multiple alignments of protein sequences.” Nucl. Acids Res. 32(suppl_2): W64-68.

Agarwal, K. L., H. Buchi, et al. (1970). “Total Synthesis of the Gene for an Alanine Transfer Ribonucleic Acid from Yeast.” Nature 227(5253): 27-34.

Agarwal, R., T. Binz, et al. (2005). “Analysis of Active Site Residues of Botulinum Neurotoxin E by Mutational, Functional, and Structural Studies: Glu335Gln Is an Apoenzyme.” Biochemistry 44(23): 8291-8302.

Alcami, A. (2003). Viral mimicry of cytokines, chemokines and their receptors. Nature Reviews Immunology, Nature Publishing Group. 3: 36.

Anandalakshmi, R., G. J. Pruss, et al. (1998). “A viral suppressor of gene silencing in plants.” Proceedings of the National Academy of Sciences of the United States of America 95(22): 13079-13084.

Ben-David, M., O. Noivirt-Brik, et al. (2009). ”Assessment of CASP8 structure predictions for template free targets.” Proteins: Structure, Function, and Bioinformatics 77(S9): 50-65.

Bent, A. F. and D. Mackey (2007). “Elicitors, Effectors, and R Genes: The New Paradigm and a Lifetime Supply of Questions.” Annual Review of Phytopathology 45(1): 399-436.

Berger, K. M., W. Pinard, et al. (2009). Minimizing the Risks of Synthetic DNA: Scientists’ Views on the U.S. Government’s Guidance on Synthetic Genomics.

Black, D. S. and J. B. Bliska (2000). “The RhoGAP activity of the Yersinia pseudotuberculosis cytotoxin YopE is required for antiphagocytic function and virulence.” Molecular Microbiology 37(3): 515-527.

Bowie, A. G. and L. Unterholzner (2008). “Viral evasion and subversion of pattern-recognition receptor signalling.” Nat Rev Immunol 8(12): 911-922.

Breeze, Budowle, et al., Eds. (2005). Microbial Forensics, Elsevier Academic Press.

Brigneti, G., O. Voinnet, et al. (1998). “Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana.” EMBO J 17(22): 6739-6746.

Buller, R. M. and G. J. Palumbo (1991). “Poxvirus pathogenesis.” Microbiol. Mol. Biol. Rev. 55(1): 80-122.

Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
×

Casadevall, A. and D. A. Relman (2010). “Microbial threat lists: obstacles in the quest for biosecurity?” Nature Reviews Microbiology 8(2): 149-154.

CDC (1974). Classification of Etiologic Agents on the Basis of Hazard. U. S. N. C. D. Center. Atlanta, GA.

CDC (2005). Notification of Exclusion of Attenuated Strains.

CDC/NIH. (2007). “Biosafety in microbiological and biomedical laboratories.” from http://purl. access.gpo.gov/GPO/LPS121160.

Cello, J., A. V. Paul, et al. (2002). “Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template.” Science 297(5583): 1016-1018.

Chang, J. H., J. M. Urbach, et al. (2005). “A High-Throughput, Near-Saturating Screen for Type III Effector Genes from Pseudomonas syringae.” Proceedings of the National Academy of Sciences of the United States of America 102(7): 2549-2554.

Chang, M. C. Y., R. A. Eachus, et al. (2007). “Engineering Escherichia coli for production of functionalized terpenoids using plant P450s.” Nat Chem Biol 3(5): 274-277.

Commission on the Prevention of WMD Proliferation and Terrorism. (2008). World at Risk : the report of the Commission on the Prevention of WMD Proliferation and Terrorism. New York, Vintage Books.

Couch, B. C., I. Fudal, et al. (2005). “Origins of Host-Specific Populations of the Blast Pathogen Magnaporthe oryzae in Crop Domestication With Subsequent Expansion of Pandemic Clones on Rice and Weeds of Rice.” Genetics 170(2): 613-630.

Damon, I. K. (2007). Genus Orthopoxvirus: Variola virus. Poxviruses: 47-64.

DHHS (1979). “Interstate shipment of etiologic agents; proposed rule.” Federal Register 44(226): 66853-5.

DHHS (1996). 42 CFR Part 72: Additional Requirements for Facilities Transferring or Receiving Select Agents, Federal Register. 61: 55190-55200.

DHHS (2002). Public Health Security and Bioterrorism Preparedness and Response Act of 2002.

DHHS (2005). 42 CFR 72 and 73 and 42 CFR Part 1003: Possession, Use, and Transfer of Select Agents and Toxins; Final Rule, Federal Register. 70: 12294-13325.

DHHS (2009). Screening Framework Guidance for Synthetic Double-Stranded DNA Providers, Federal Register. 74.

DHS (2006). Bioterrorism Risk Assessment. Fort Detrick, MD, Biological Threat Characterization Center of the National Biodefense Analysis and Countermeasures Center.

Dias, M. B., L. Reyes-Gonzalez, et al. (2010). “Effects of the USA PATRIOT ACT and the 2002 Bioterrorism Preparedness Act on select agent research in the United States.” Proceedings of the National Academy of Sciences 107: 9556-9561.

Doolittle, R. (1981). “Similar amino acid sequences: chance or common ancestry?” Science 214(4517): 149-159.

Dunoyer, P., C.-H. Lecellier, et al. (2004). “Probing the MicroRNA and Small Interfering RNA Pathways with Virus-Encoded Suppressors of RNA Silencing.” Plant Cell 16(5): 1235-1250.

Dymond, J. S., L. Z. Scheifele, et al. (2009). “Teaching Synthetic Biology, Bioinformatics and Engineering to Undergraduates: The Interdisciplinary Build-a-Genome Course.” Genetics 181(1): 13-21.

Esposito, J. J., S. A. Sammons, et al. (2006). “Genome Sequence Diversity and Clues to the Evolution of Variola (Smallpox) Virus.” Science: 1125134.

Fallman, M., K. Andersson, et al. (1995). “Yersinia pseudotuberculosis inhibits Fc receptor-mediated phagocytosis in J774 cells.” Infection and Immunity 63(8): 3117-3124.

Fishman, J. A. (2007). “Infection in Solid-Organ Transplant Recipients.” N Engl J Med 357(25): 2601-2614.

Flannagan, R. S., G. Cosio, et al. (2009). ”Antimicrobial mechanisms of phagocytes and bacterial evasion strategies.” Nat Rev Micro 7(5): 355-366.

Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
×

Fleishman, S. J., O. Yifrach, et al. (2004). ”An evolutionarily conserved network of amino acids mediates gating in voltage-dependent potassium channels.” Journal of Molecular Biology 340(2): 307-318.

Frankel, A., P. Welsh, et al. (1990). “Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomes.” Mol. Cell. Biol. 10(12): 6257-6263.

Fujii, N., K. Kimura, et al. (1992). “A zinc-protease specific domain in botulinum and tetanus neurotoxins.” Toxicon 30(11): 1486-1488.

Genomesonline. (2009). “GOLD: Genomes OnLine Databases.”, from http://www.genomesonline. org/gold.cgi/.

Gibson, D. G., J. I. Glass, et al. (2010). “Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome.” Science 329(5987): 52-56.

Gillespie, J. J., N. C. Ammerman, et al. (2009). “Louse- and flea-borne rickettsioses: biological and genomic analyses.” Vet. Res. 40(2).

Graff, J. W., K. Ettayebi, et al. (2009). “Rotavirus NSP1 Inhibits NFκB Activation by Inducing Proteasome-Dependent Degradation of Î2-TrCP: A Novel Mechanism of IFN Antagonism.” PLoS Pathog 5(1): e1000280.

Gubser, C., S. Hue, et al. (2004). “Poxvirus genomes: a phylogenetic analysis.” J Gen Virol 85(1): 105-117.

Haber, E. and C. B. Anfinsen (1962). “Side-chain Interactions Governing the Pairing of Half-cystine Residues in Ribonuclease.” Journal of Biological Chemistry 237(6): 1839-1844.

Hannenhalli, S. S. and R. B. Russell (2000). “Analysis and prediction of functional sub-types from protein sequence alignments.” Journal of Molecular Biology 303(1): 61-76.

He, Y., D. C. Yeh, et al. (2005). “Solution NMR Structures of IgG Binding Domains with Artificially Evolved High Levels of Sequence Identity but Different Folds.” Biochemistry 44(43): 14055-14061.

Hussain, S., S. Perlman, et al. (2008). ”Severe Acute Respiratory Syndrome Coronavirus Protein 6 Accelerates Murine Hepatitis Virus Infections by More than One Mechanism.” J. Virol. 82(14): 7212-7222.

Iyer, L. M., S. Balaji, et al. (2006). “Evolutionary genomics of nucleo-cytoplasmic large DNA viruses.” Virus Research 117(1): 156-184.

Janin, J. (2005). “Assessing predictions of protein-protein interaction: The CAPRI experiment.” Protein Science 14(2): 278-283.

Joiner, K., E. Brown, et al. (1983). “Studies on the mechanism of bacterial resistance to complement-mediated killing. III. C5b-9 deposits stably on rough and type 7 S. pneumoniae without causing bacterial killing.” J Immunol 130(2): 845-849.

Keedy, D. A., C. J. Williams, et al. (2009). “The other 90% of the protein: Assessment beyond the Calphas for CASP8 template-based and high-accuracy models.” Proteins: Structure, Function, and Bioinformatics 77(S9): 29-49.

Kim, D. W., G. Lenzen, et al. (2005). “The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes.” Proceedings of the National Academy of Sciences of the United States of America 102(39): 14046-14051.

Kim, Y., D. Misna, et al. (1992). ”Structure of a ricin mutant showing rescue of activity by a non-catalytic residue.” Biochemistry 31(12): 3294-3296.

Kingsley, R. A. and A. J. Bäumler (2000). “Host adaptation and the emergence of infectious disease: the Salmonella paradigm.” Molecular Microbiology 36(5): 1006-1014.

Konkel, M. E. and K. Tilly (2000). “Temperature-regulated expression of bacterial virulence genes.” Microbes and Infection 2(2): 157-166.

Kortepeter, M. G. and G. W. Parker (1999). “Potential biological weapons threats.” Emerging infectious diseases 5(4).

Kuhlman, B., G. Dantas, et al. (2003). ”Design of a Novel Globular Protein Fold with Atomic-Level Accuracy.” Science 302(5649): 1364-1368.

Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
×

Kwok, R. (2010). “Five hard truths for synthetic biology.” Nature. 463(7279): 288.

Lacy, D. B., W. Tepp, et al. (1998). “Crystal structure of botulinum neurotoxin type A and implications for toxicity.” Nat Struct Biol 5(10): 898-902.

Lambris, J. D., D. Ricklin, et al. (2008). ”Complement evasion by human pathogens.” Nat Rev Micro 6(2): 132-142.

Lartigue, C., S. Vashee, et al. (2009). “Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast.” Science 325(5948): 1693-1696.

Lee, S. K., H. Chou, et al. (2008). “Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels.” Current Opinion in Biotechnology 19(6): 556-563.

Lefkowitz, E. J., C. Wang, et al. (2006). “Poxviruses: past, present and future.” Virus Research 117(1): 105-118.

Li, Y., D. S. Carroll, et al. (2007). “On the origin of smallpox: Correlating variola phylogenics with historical smallpox records.” Proceedings of the National Academy of Sciences 104(40): 15787-15792.

Lindesmith, L., C. Moe, et al. (2003). “Human susceptibility and resistance to Norwalk virus infection.” Nat Med 9(5): 548-553.

López, G., I. Ezkurdia, et al. (2009). “Assessment of ligand binding residue predictions in CASP8.” Proteins: Structure, Function, and Bioinformatics 77(S9): 138-146.

Lu, R., A. Folimonov, et al. (2004). “Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome.” Proceedings of the National Academy of Sciences of the United States of America 101(44): 15742-15747.

Matson, J. S., J. H. Withey, et al. (2007). “Regulatory Networks Controlling Vibrio cholerae Virulence Gene Expression.” Infect. Immun. 75(12): 5542-5549.

Maurelli, A. T., R. E. Fernandez, et al. (1998). “”Black holes” and bacterial pathogenicity: A large genomic deletion that enhances the virulence of Shigella spp. and enteroinvasive Escherichia coli.” Proceedings of the National Academy of Sciences of the United States of America 95(7): 3943-3948.

Maurer, S. M., M. Fischer, et al. (2009). Making Commercial Biology Safer: What the Gene Synthesis Industry Has Learned About Screening Customers and Orders.

McAuley, J. L., K. Zhang, et al. ”The Effects of Influenza A Virus PB1-F2 Protein on Polymerase Activity Are Strain Specific and Do Not Impact Pathogenesis.” J. Virol. 84(1): 558-564.

McLeod, S. M., H. H. Kimsey, et al. (2005). “CTX; and Vibrio cholerae: exploring a newly recognized type of phage-host cell relationship.” Molecular Microbiology 57(2): 347-356.

Mohamed, M. R., M. M. Rahman, et al. (2009). “Proteomic screening of variola virus reveals a unique NF-κB inhibitor that is highly conserved among pathogenic orthopoxviruses.” Proceedings of the National Academy of Sciences 106(22): 9045-9050.

Moss, B. (2007). Poxviridae: The viruses and their replication. Fields Viology. D. M. Knipe and P. M. Howley, Lippincott, William and Wilkins: 2905-2946.

Moult, J. (2005). “A decade of CASP: progress, bottlenecks and prognosis in protein structure prediction.” Current Opinion in Structural Biology 15(3): 285-289.

Moulton, E. A., J. P. Atkinson, et al. (2008). “Surviving Mousepox Infection Requires the Complement System.” PLoS Pathog 4(12): e1000249.

Munishkin, A. and I. G. Wool (1995). “Systematic Deletion Analysis of Ricin A-Chain Function.” Journal of Biological Chemistry 270(51): 30581-30587.

NBACC (2009). National Biodefense Analysis & Countermeasures Center Strategic Plan. NBACC.

Neish, A. (2004). “Bacterial inhibition of eukaryotic pro-inflammatory pathways.” Immunologic Research 29(1): 175-185.

NIH (1976). Guidelines for research involving recombinant DNA molecules : NIH guidelines, U.S. Dept. of Health, Education, and Welfare, Public Health Service.

Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
×

NRC (2004). Biotechnology research in an age of terrorism. Washington, D.C., National Academies Press.

NRC (2008). Department of Homeland Security bioterrorism risk assessment: a call for change. Washington, D. C., National Academies Press.

NRC (2009a). Live Variola virus: Considerations for Continuing Research. Washington, DC, National Academies Press.

NRC (2009b). Responsible Research with Biological Select Agents and Toxins. Washington, DC, National Academies Press.

NRC (2009c). A survey of attitudes and actions on dual use research in the life sciences : a collaborative effort of the National Research Council and the American Association for the Advancement of Science. Washington, D.C., National Academies Press.

NSABB (2006) “Addressing Biosecurity Concerns Related to the Synthesis of Select Agents.”

NSABB (2009a). Enhancing Personnel Reliability among Individuals with Access to Select Agents.

NSABB (2009b). Report of the Working Group on Strengthening the Biosecurity of the United States.

NSC (2009). National strategy for countering biological threats. Washington, D.C., National Security Council.

Odom, M. R., R. Curtis Hendrickson, et al. (2009). “Poxvirus protein evolution: Family wide assessment of possible horizontal gene transfer events.” Virus Research 144(1-2): 233-249.

OECD (Organisation for Economic Co-operation and Development) (2007). OECD Best Practice Guidelines on Biosecurity for BRCs (Biological Resource Centers). Paris: OECD. Available at ,http://www.oecd.org/dataoecd/6/27/38778261.pdf>.

Park, I. H., D. G. Pritchard, et al. (2007). “Discovery of a New Capsular Serotype (6C) within Serogroup 6 of Streptococcus pneumoniae.” J. Clin. Microbiol. 45(4): 1225-1233.

Paya, C. V. (1993). “Fungal Infections in Solid-Organ Transplantation.” Clinical Infectious Diseases 16(5): 677-688.

Pesenti, P. T. (2009). DHS S&T Bioterrorism Risk Assessment (BTRA).

Preston, R. (1998). Annals of Warfare: The Bioweaponeers. The New Yorker: 52-65.

Qian, B., S. Raman, et al. (2007). “High-resolution structure prediction and the crystallographic phase problem.” Nature 450(7167): 259-264.

Qiu, W., J.-W. Park, et al. (2007). “Tombusvirus P19-Mediated Suppression of Virus-Induced Gene Silencing Is Controlled by Genetic and Dosage Features That Influence Pathogenicity.” Molecular Plant-Microbe Interactions 15(3): 269-280.

Raman, S., R. Vernon, et al. (2009). “Structure prediction for CASP8 with all-atom refinement using Rosetta.” Proteins: Structure, Function, and Bioinformatics 77(S9): 89-99.

Read, T. D., S. N. Peterson, et al. (2003). “The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria.” Nature 423(6935): 81-86.

Rotz, L. D., A. S. Khan, et al. (2002). “Public health assessment of potential biological terrorism agents.” Emerging infectious diseases 8(2): 225-30.

Schnoes, A. M., S. D. Brown, et al. (2009). “Annotation Error in Public Databases: Misannotation of Molecular Function in Enzyme Superfamilies.” PLoS Comput Biol 5(12): e1000605.

Schorey, J. S., M. C. Carroll, et al. (1997). “A Macrophage Invasion Mechanism of Pathogenic Mycobacteria.” Science 277(5329): 1091-1093.

Schwan, T. G. and J. Piesman (2002). “Vector Interactions and Molecular Adaptations of Lyme Disease and Relapsing Fever Spirochetes Associated with Transmission by Ticks.” Emerg Infect Dis 8(2): 115-21.

Sing, A., D. Reithmeier-Rost, et al. (2005). “A hypervariable N-terminal region of Yersinia LcrV determines Toll-like receptor 2-mediated IL-10 induction and mouse virulence.” Proceedings of the National Academy of Sciences of the United States of America 102(44): 16049-16054.

Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
×

Sodhi, A., S. Montaner, et al. (2004). “Viral hijacking of G-protein-coupled-receptor signalling networks.” Nat Rev Mol Cell Biol 5(12): 998-1012.

Soosaar, J. L. M., T. M. Burch-Smith, et al. (2005). “Mechanisms of plant resistance to viruses.” Nat Rev Micro 3(10): 789-798.

Stukenbrock, E. H., S. Banke, et al. (2007). ”Origin and Domestication of the Fungal Wheat Pathogen Mycosphaerella graminicola via Sympatric Speciation.” Mol Biol Evol 24(2): 398-411.

Stukenbrock, E. H. and B. A. McDonald (2008). “The Origins of Plant Pathogens in Agro-Ecosystems.” Annual Review of Phytopathology 46(1): 75-100.

Sumby, P., S. Zhang, et al. (2008). “A Chemokine-Degrading Extracellular Protease Made by Group A Streptococcus Alters Pathogenesis by Enhancing Evasion of the Innate Immune Response.” Infect. Immun.: IAI. 01354-07.

Sutton, V. (2009). “Survey Finds Biodefense Researcher Anxiety - Over Inadvertently Violating Regulations.” Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science 7(2).

The White House. (2004). HSPD-10: Biodefense in the 21st Century.

Tscharke, D. C., P. C. Reading, et al. (2002). “Dermal infection with vaccinia virus reveals roles for virus proteins not seen using other inoculation routes.” J Gen Virol 83(8): 1977-1986.

Tucker, J. (2003). “Preventing the Misuse of Pathogens: The Need for Global Biosecurity.” Arms Control Today 33.

Upton, C., S. Slack, et al. (2003). “Poxvirus Orthologous Clusters: toward Defining the Minimum Essential Poxvirus Genome.” J. Virol. 77(13): 7590-7600.

van der Does, H. C. and M. Rep (2007). “Virulence Genes and the Evolution of Host Specificity in Plant-Pathogenic Fungi.” Molecular Plant-Microbe Interactions 20(10): 1175-1182.

Vivian, A. and M. J. Gibbon (1997). “Avirulence genes in plant-pathogenic bacteria: signals or weapons?” Microbiology 143(3): 693-704.

Voinnet, O., C. Lederer, et al. (2000). “A Viral Movement Protein Prevents Spread of the Gene Silencing Signal in Nicotiana benthamiana.” Cell 103(1): 157-167.

Walport, M. J. (2001a). “Complement- First of Two Parts.” N Engl J Med 344(14): 1058-1066.

Walport, M. J. (2001b). “Complement- Second of Two Parts.” N Engl J Med 344(15): 1140-1144.

WHO (World Health Organization) (2004). Laboratory Biosafety Manual, 3rd ed. WHO/CDS/CSR/LYO/2004.11 Geneva: WHO. Available at <http://www.who.int/csr/resources. publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/>.

Working Group on Strengthening the Biosecurity of the United States. (2009). Report of the Working Group on Strengthening the Biosecurity of the United States.

Wu, K., W. Li, et al. (2009). “Crystal structure of NL63 respiratory coronavirus receptor-binding domain complexed with its human receptor.” Proceedings of the National Academy of Sciences 106(47): 19970-19974.

Yoshida, N., K. Oeda, et al. (2001). “Protein function: Chaperonin turned insect toxin.” Nature 411(6833): 44-44.

Zhang, J., Q. Wang, et al. “MUFOLD: A new solution for protein 3D structure prediction.” Proteins: Structure, Function, and Bioinformatics 78(5): 1137-1152.

Zychlinsky, A., B. Kenny, et al. (1994). “IpaB mediates macrophage apoptosis induced by Shigella flexneri.” Molecular Microbiology 11(4): 619-627.

Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
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Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
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Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
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Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
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Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
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Suggested Citation:"References." National Research Council. 2010. Sequence-Based Classification of Select Agents: A Brighter Line. Washington, DC: The National Academies Press. doi: 10.17226/12970.
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Select Agents are defined in regulations through a list of names of particularly dangerous known bacteria, viruses, toxins, and fungi. However, natural variation and intentional genetic modification blur the boundaries of any discrete Select Agent list based on names. Access to technologies that can generate or 'synthesize' any DNA sequence is expanding, making it easier and less expensive for researchers, industry scientists, and amateur users to create organisms without needing to obtain samples of existing stocks or cultures. This has led to growing concerns that these DNA synthesis technologies might be used to synthesize Select Agents, modify such agents by introducing small changes to the genetic sequence, or create entirely new pathogens. Amid these concerns, the National Institutes of Health requested that the Research Council investigate the science and technology needed to replace the current Select Agent list with an oversight system that predicts if a DNA sequence could be used to produce an organism that should be regulated as a Select Agent.

A DNA sequence-based system to better define when a pathogen or toxin is subject to Select Agent regulations could be developed. This could be coupled with a 'yellow flag' system that would recognize requests to synthesize suspicious sequences and serve as a reference to anyone with relevant questions, allowing for appropriate follow-up.

Sequence-Based Classification of Select Agents finds that replacing the current list of Select Agents with a system that could predict if fragments of DNA sequences could be used to produce novel pathogens with Select Agent characteristics is not feasible. However, it emphasized that for the foreseeable future, any threat from synthetic biology and synthetic genomics is far more likely to come from assembling known Select Agents, or modifications of them, rather than construction of previously unknown agents. Therefore, the book recommends modernizing the regulations to define Select Agents in terms of their gene sequences, not by their names, and called this 'sequence-based classification.'

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