APPENDIX TABLE 1 Fatty Acid Composition (%) of Some Common Fats Used in Rodent Diets
|
Fatty Acidsa |
Canola Oilb |
Cocoa Butterc |
Coconut (hydrogenated-96°) Oild |
Corn Oile |
Cottonseed Oil |
Fish (Menhaden) Oilf |
Tallow |
Linseed Oilg |
Olive Oilh |
Peanut Oil |
Sunflower Oili |
Soybean Oilj |
|
8:0 |
|
|
6.8 |
|
|
|
|
|
|
|
|
|
|
10:0 |
|
|
5.8 |
|
|
|
|
|
|
|
|
|
|
12:0 |
|
|
46.7 |
|
|
0.2 |
0.1 |
|
|
|
|
|
|
14:0 |
|
0.1 |
18.5 |
0.1 |
0.8 |
7.3 |
3.4 |
|
|
0.1 |
0.1 |
0.1 |
|
14:1 |
|
|
0.2 |
|
|
|
0.7 |
|
|
|
|
|
|
15:0 |
|
|
|
|
|
|
0.6 |
|
|
|
|
|
|
16:0 |
3.9 |
25.4 |
9.5 |
11.0 |
23.4 |
19.0 |
24.8 |
5.3 |
12.0 |
10.9 |
6.7 |
10.5 |
|
16:1 |
0.2 |
0.2 |
|
0.1 |
0.6 |
9.1 |
3.4 |
|
1.2 |
0.1 |
0.1 |
|
|
17:0 |
|
|
|
|
|
0.9 |
1.3 |
|
0.1 |
0.1 |
|
0.1 |
|
17:1 |
|
|
|
|
|
|
0.8 |
|
0.1 |
0.1 |
|
|
|
18:0 |
2.0 |
33.2 |
3.1 |
2.2 |
2.4 |
4.2 |
18.6 |
4.1 |
2.3 |
2.5 |
4.8 |
3.9 |
|
18:1(n-9) |
61.8 |
32.6 |
7.1 |
25.5 |
17.9 |
13.2 |
42.2 |
20.2 |
72.9 |
45.8 |
18.7 |
22.6 |
|
18:2(n-6) |
20.0 |
2.8 |
1.9 |
59.5 |
53.6 |
1.3 |
2.8 |
12.7 |
9.6 |
33.0 |
67.3 |
54.2 |
|
18:3(n-3) |
9.3 |
0.1 |
0.1 |
1.0 |
0.5 |
1.3 |
0.8 |
53.3 |
1.0 |
0.5 |
1.1 |
7.7 |
|
20:0 |
0.4 |
|
|
0.4 |
0.3 |
0.4 |
0.3 |
|
0.1 |
1.3 |
0.2 |
0.3 |
|
20:1(n-11) |
1.5 |
|
|
0.3 |
|
2.0 |
|
|
0.3 |
1.0 |
0.2 |
|
|
20:4(n-6) |
|
|
|
|
|
0.2 |
|
|
|
|
|
|
|
20:5(n-3) |
|
|
|
|
|
11.0 |
|
|
|
|
|
|
|
22:0 |
0.4 |
|
|
|
|
0.2 |
|
|
0.1 |
2.9 |
0.6 |
0.3 |
|
22:1(n-11) |
0.5 |
|
|
|
|
0.6 |
|
|
|
|
|
|
|
22:6(n-3) |
|
|
|
|
|
9.1 |
|
|
|
|
|
|
|
Other |
0.0 |
5.6 |
0.3 |
0.0 |
0.5 |
20.0 |
0.2 |
4.4 |
0.3 |
1.7 |
0.3 |
0.2 |
|
n-6:n-3 |
2.2 |
28.0 |
19.0 |
59.5 |
107.2 |
0.1 |
3.5 |
0.2 |
9.6 |
66.0 |
61.2 |
7.0 |
|
NOTE: Unless otherwise indicated, fatty acid composition was supplied by E. Wayne Emmons, AOCS Chromatography Chairman, 1991 (personal communication). a 14:1 and 17:1 contain double bond in n-9 or n-11 position, exact position unknown. b Abundance of n-3 and monounsaturated fat. c Abundance of long-chain saturated and monounsaturated fatty acid composition from U.S. Department of Agriculture Handbook No. 8-4 (1979). d A highly saturated fat; contains predominantly medium-chain triglycerides and very little trans fatty acid. e Good source of essential fatty acid, n-6; suggested for use in the AIN-76 rodent reference diet (American Institute of Nutrition, 1977). f Contains large amounts of very long chain n-3 fatty acids; usually needs to be supplemented with an n-6 containing oil for use in a rodent diet (fatty acid composition from Ackman, R. G., 1982). g Contains large quantity n-3 fatty acid; fatty acid composition from U.S. Department of Agriculture Handbook No. 8-4 (1979). h Contains large quantity of monounsaturated fat. i Greatest source of n-6 fatty acid of these fat sources. j Contains significant quantity of n-6 and n-3 fatty acid; partially hydrogenated soybean oil will also contain some trans fatty acids. |
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APPENDIX TABLE 2 Amino Acid Composition (mg/g nitrogen) of Purified Proteins Used in Laboratory Animal Diets
APPENDIX TABLE 3 Molecular Weights of Vitamins
APPENDIX TABLE 4 Conversion Factors
