Appendix



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Nutrient Requirements of Laboratory Animals: Fourth Revised Edition, 1995 Appendix

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Nutrient Requirements of Laboratory Animals: Fourth Revised Edition, 1995 This page in the original is blank.

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Nutrient Requirements of Laboratory Animals: Fourth Revised Edition, 1995 APPENDIX TABLE 1 Fatty Acid Composition (%) of Some Common Fats Used in Rodent Diets Fatty Acidsa Canola Oilb Cocoa Butterc Coconut (hydrogenated-96°) Oild Corn Oile Cottonseed Oil Fish (Menhaden) Oilf Tallow Linseed Oilg Olive Oilh Peanut Oil Sunflower Oili Soybean Oilj 8:0     6.8                   10:0     5.8                   12:0     46.7     0.2 0.1           14:0   0.1 18.5 0.1 0.8 7.3 3.4     0.1 0.1 0.1 14:1     0.2       0.7           15:0             0.6           16:0 3.9 25.4 9.5 11.0 23.4 19.0 24.8 5.3 12.0 10.9 6.7 10.5 16:1 0.2 0.2   0.1 0.6 9.1 3.4   1.2 0.1 0.1   17:0           0.9 1.3   0.1 0.1   0.1 17:1             0.8   0.1 0.1     18:0 2.0 33.2 3.1 2.2 2.4 4.2 18.6 4.1 2.3 2.5 4.8 3.9 18:1(n-9) 61.8 32.6 7.1 25.5 17.9 13.2 42.2 20.2 72.9 45.8 18.7 22.6 18:2(n-6) 20.0 2.8 1.9 59.5 53.6 1.3 2.8 12.7 9.6 33.0 67.3 54.2 18:3(n-3) 9.3 0.1 0.1 1.0 0.5 1.3 0.8 53.3 1.0 0.5 1.1 7.7 20:0 0.4     0.4 0.3 0.4 0.3   0.1 1.3 0.2 0.3 20:1(n-11) 1.5     0.3   2.0     0.3 1.0 0.2   20:4(n-6)           0.2             20:5(n-3)           11.0             22:0 0.4         0.2     0.1 2.9 0.6 0.3 22:1(n-11) 0.5         0.6             22:6(n-3)           9.1             Other 0.0 5.6 0.3 0.0 0.5 20.0 0.2 4.4 0.3 1.7 0.3 0.2 n-6:n-3 2.2 28.0 19.0 59.5 107.2 0.1 3.5 0.2 9.6 66.0 61.2 7.0 NOTE: Unless otherwise indicated, fatty acid composition was supplied by E. Wayne Emmons, AOCS Chromatography Chairman, 1991 (personal communication). a 14:1 and 17:1 contain double bond in n-9 or n-11 position, exact position unknown. b Abundance of n-3 and monounsaturated fat. c Abundance of long-chain saturated and monounsaturated fatty acid composition from U.S. Department of Agriculture Handbook No. 8-4 (1979). d A highly saturated fat; contains predominantly medium-chain triglycerides and very little trans fatty acid. e Good source of essential fatty acid, n-6; suggested for use in the AIN-76 rodent reference diet (American Institute of Nutrition, 1977). f Contains large amounts of very long chain n-3 fatty acids; usually needs to be supplemented with an n-6 containing oil for use in a rodent diet (fatty acid composition from Ackman, R. G., 1982). g Contains large quantity n-3 fatty acid; fatty acid composition from U.S. Department of Agriculture Handbook No. 8-4 (1979). h Contains large quantity of monounsaturated fat. i Greatest source of n-6 fatty acid of these fat sources. j Contains significant quantity of n-6 and n-3 fatty acid; partially hydrogenated soybean oil will also contain some trans fatty acids.

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Nutrient Requirements of Laboratory Animals: Fourth Revised Edition, 1995 APPENDIX TABLE 2 Amino Acid Composition (mg/g nitrogen) of Purified Proteins Used in Laboratory Animal Diets Amino Acid Acid Casein ANRC Casein Lactalbumin Whey Protein Concentrate Soybean Protein Isolate Alanine 188 188 369 331 269 Arginine 231 231 188 175 475 Asparagine 431 469 769 681 725 Cystine 25 25 200 169 81 Glutamic acid 1,306 1,563 925 1,094 1,194 Glycine 113 125 144 125 263 Histidine 181 200 131 131 163 Isoleucine 288 313 319 338 306 Leucine 569 594 875 744 513 Lysine 481 488 669 588 394 Methionine 181 200 131 156 81 Phenylalanine 319 331 244 219 325 Proline 650 706 319 331 319 Serine 363 381 313 338 325 Threonine 269 300 369 413 238 Tryptophan 75 75 144 125 81 Tyrosine 344 369 250 206 238 Valine 356 388 313 319 313 NOTE: Data was obtained from New Zealand Milk Products, Inc., and Protein Technologies International. All calculations assume protein contained 16 percent nitrogen. APPENDIX TABLE 3 Molecular Weights of Vitamins Compound Molecular Weight Vitamin A   Retinol 286.46 3,4-Dehydroretinol (vitamin A2) 284.44 Retinaldehyde (Retinal) 284.44 Retinoic acid 300.44 Retinyl acetate 328.50 Retinyl palmitate 524.88 Retinyl stearate 552.93 Retinyl oleate 550.91 β-Carotene 536.89 Vitamin D   Ergocalciferol (vitamin D2) 396.66 Cholecalciferol (vitamin D3) 384.65 25-Hydroxycholecalciferol 400.65 1,25-Dihydroxycholecalciferol 416.65 Vitamin E   α-Tocopherol 430.72 α-Tocopheryl acetate 472.76 β-Tocopherol 416.69 γ-Tocopherol 416.69 δ-Tocopherol 402.66 α-Tocotrienol 424.67 Vitamin K   Phylloquinone (vitamin K1) 450.71 Menaquinone-4 444.66 Menaquinone-7 649.02 Menaquinone-8 717.14 Meanquinone-9 785.26 Menadione 172.18 Menadione sodium bisulfite 276.25 Menadione dimethylpyridinol   bisulfite 378.41 Vitamin C   Ascorbic acid 176.13 Dehydroascorbic acid 174.11 Biotin   Biotin 244.32 Choline   Choline (free base) 104.17 Choline bitartrate 253.25 Choline chloride 139.63 Folate   Folic acid 441.41 Niacin   Nicotinic acid 123.11 Nicotinamide 122.13 Pantothenic acid   Pantothenic acid 219.24 Sodium pantothenate 241.22 Calcium pantothenate 476.54 Calcium pantothenate monohydrate 494.56 Vitamin B6   Pyridoxine hydrochloride 205.64 Pyridoxal 167.17 Pyridoxal hydrochloride 203.63 Pyridoxamine dihydrochloride 241.12 Pyridoxal phosphate 247.15 Riboflavin   Riboflavin 376.37 Sodium riboflavin phosphate   (FMN) 478.33 Flavin adenine dinucleotide (FAD) 785.56 Thiamin   Thiamin hydrochloride 337.27 Thiamin diphosphate chloride 460.77 Thiamin mononitrate 327.36 Thiamin triphosphate 504.29 Vitamin B12   Cyanocobalamin 1,355.39 Hydroxocobalamin 1,346.38 5'-deoxyadenosylcobalamin 1,579.61 NOTE: Data are based on atomic weights from Pure and Applied Chemistry (1991; 63:978-979).

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Nutrient Requirements of Laboratory Animals: Fourth Revised Edition, 1995 APPENDIX TABLE 4 Conversion Factors Element Traditional Unit Converted Equivalent Vitamin A 1 international unit 0.3 µg retinol   1 international unit 0.34 µg retinyl acetate   1 international unit 0.55 µg retinyl palmitate   1 international unit 0.6 µg β-carotene   1 retinol equivalent 1 µg retinol   1 retinol equivalent 6 µg β-carotene   1 retinol equivalent 12 µg other provitamin A carotenoids   1 retinol equivalent 3.33 IU vitamin A activity from retinol   1 retinol equivalent 10 IU vitamin A activity from β-carotene Vitamin D 1 international unit 25 ng cholecalciferol (vitamin D3) Vitamin E 1 international unit 1 mg all-rac-α-tocopheryl acetate   1 international unit 0.74 mg RRR-α-tocopheryl acetate   1 international unit 0.91 mg all-rac-α-tocopherol   1 international unit 0.67 mg RRR-α-tocopherol Thiamin 1 international unit 3 µg thiamin hydrochloride NOTE: These terms are now obsolete; the preferred expression is the molar concentration. SOURCE: John Edgar Smith, Penn State University, personal communication, 1994.