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Nutrient Requirements of Laboratory Animals: Fourth Revised Edition, 1995
TABLE 4-1 Estimated Nutrient Requirements for Growth for Guinea Pigs
Adequate; dietary deficiency has not been produced
Ascorbic acid
mg
200.0
Mannering, 1949
Biotin (d-biotin)
mg
0.2
Adequate; simple dietary deficiency has not been produced
Choline (choline bitartrate)
mg
1,800
Reid, 1955
Folic acid
mg
3.0-6.0
Mannering, 1949; Reid, 1954a; Reid et al., 1956; Woodruff et al., 1953
Niacin
mg
10.0
Reid, 1961
Pantothenic acid (Ca-d-pantothenate)
mg
20.0
Reid and Briggs, 1954
Pyridoxine
mg
2.0-3.0
Reid, 1964
Riboflavin
mg
3.0
Estimated
Slanetz, 1943
Thiamin (thiamin-HCl)
mg
2.0
Liu et al., 1967; Reid and Bieri, 1967
NOTE: Nutrient requirements are expressed on an as-fed basis for diets containing 10% moisture; 2.8–3.5 kcal ME/g (11.7–14.6 kJ ME/g) and should be adjusted for diets of differing moisture and energy concentrations. Unless otherwise specified, the listed nutrient concentrations represent minimal requirements and do not include a margin of safety. Higher concentrations for many nutrients may be warranted in natural-ingredient diets.
a See text. Growth is equivalent with 300 g casein plus 3 g L-arginine per kg or 200 g soybean protein plus 10 g DL-methionine per kg.
b The quantities reflect a 20% adjustment for efficiency of utilization for maximum growth. See text for discussion.
c Cystine may replace 40%.
d Tyrosine may replace 50%.
e Mixture of L-alanine, L-asparagine·H2O, L-aspartic acid, L-glutamic acid, sodium glutamate, glycine, L-proline and L-serine. See text and Typpo et al. (1990b).
f Minerals measured in mg/kg correspond to ppm.
g Iodine, molybdenum, and selenium are measured in µg/kg, corresponding to ppb.
h Equivalent to 21,960 IU/kg. β-Carotene requirement measured is equivalent to 47,425 IU/kg.
i Equivalent to 1,000 IU/kg.
j Equivalent to 40 IU/kg. Higher concentrations may be required if high-fat diets are used.
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