Frontiers of Engineering Reports on Leading-Edge Engineering From the 2000 NAE Symposium on Frontiers in Engineering (2001) / Chapter Skim
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The Human Genome Project: Elucidating Our Genetic Blueprint
The Human Genome Project: Elucidating Our Genetic Blueprint
Pages 45-53
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From page 47... ...
Since its inception, the Human Genome Project has been associated with carefully crafted, milestone-oriented goals, the most recent set being established in 1998 (Collins et al., 1998~. The first phase of the project involved constructing relatively low-resolution maps of the human genome and refining the approaches for large-scale DNA sequencing.
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From page 48... ...
Cytogenetic maps represent the appearance of chromosomes when properly stained and examined microscopically. Particularly important is the appearance of differentially staining regions (called bands)
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From page 49... ...
The development of high-quality genetic and physical maps of the human genome represented key early activities of the Human Genome Project. These goals were largely reached by the mid-199Os (Figure 2~.
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From page 50... ...
In the past decade, there have been numerous advances in fluorescencebased DNA sequencing. In parallel, researchers have gained tremendous experience through the Human Genome Project's efforts to sequence several model organisms, including the yeast S
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From page 51... ...
Sequence Reads from Random Subclones hi, Assemble Sequence Contigs Directed Finishing Directed Reads from Selected Subclones Resolve Ambiguities, Validate, ~~ and Annotate Finished Sequence BAC FIGURE 3 Shotgun DNA sequencing. The dominant genomic sequencing strategy being used in the Human Genome Project is shotgun sequencing, which consists of two major phases.
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From page 52... ...
These tools reflect both an information resource in the form of genetic maps, physical maps, and the underlying sequence of the human genome and that of several model organisms, as well as an ever-increasing number of experimental technologies that are becoming standard techniques in the armamentarium of biomedical researchers. In this regard, an exciting new "genomic revolution" has started and will forever change the way research is performed.
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From page 53... ...
1998. Shotgun sequencing of the human genome.
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