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(NAS Colloquium) Virulence and Defense in Host--Pathogen Interactions Common Features Between Plants and Animals (2001) / Chapter Skim
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Phenotypic Variation and Intracellular Parasitism by Histoplasma capsulatum
Pages 43-47

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From page 43...
... Humans and other mammals inhale aerosolized conidia and hyphal fragments, prompting a dramatic conversion of the mycelial form to budding yeasts. The phenotypic variation between mycelial and yeast forms corresponds to a complete switch in lifestyle, from a saprophytic soil-dwelling fungus to a parasitic form that is highly adapted for growth at higher temperature (37°C)
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From page 44...
... Most importantly, inverted telomeric repeats at each end of the linear plasmid help maintain the plasmid extrachromosomally and nearly eliminate ectopic integration events. For selection and disruption, the linear plasmid contains a URA5 gene, and the CBP1 gene has an internal fragment replaced by a hygromycin resistance marker (hph)
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From page 45...
... To follow the regulation of CBP1 during the transition from yeast cells to mycelia, a similar plasmid construct was randomly integrated into the chromosome to stabilize`gfp copy number from cell to cell. Expression of ~(p was monitored by fluorescence microscopy after placing a yeast culture at 25°C to trigger transition to the mycelial form (Fig.
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From page 46...
... (A) Differential interference contrast microscopy.
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From page 47...
... With the development of molecular genetic tools such as freely replicating plasmids, reporter genes, and gene disruption strategies, it is now possible to probe this organism's fascinating biology with genetic preci1. Eissenberg, L
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Key Terms

  • mycelial form
  • yeast phase
  • phenotypic variation
  • host cell
  • yeast form

  • yeast cell
  • intracellular parasitism
  • dense culture
  • fluorescence microscopy
  • differential interference

  • gene disruption
  • stationary phase
  • culture growth
  • disruption strategies
  • dimorphic fungal

  • telomeric plasmid
  • fungal pathogens
  • northern blot
  • broth culture
  • linear telomeric

  • blot analysis
  • linear plasmid
  • brightly fluorescent
  • cell walls
  • cell density

  • recombination events
  • molecular genetic
  • hyphal extensions
  • capsulatum yeast
  • interference contrast


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