(NAS Colloquium) Molecular Kinesis in Cellular Function and Plasticity (2002) / Chapter Skim
Currently Skimming:
Pages 33-40
The Chapter Skim interface presents what we've algorithmically identified as the most significant single chunk of text within every page in the chapter.
Select key terms on the right to highlight them within pages of the chapter.
From page 33... ...
and eIF4E subunits and the eIF4Gs5O-~o9o fragment of its 1,560-amino acid eIF4G subunit constitute the core of eIF4F This paper was presentecl at the Nationai Acaclemy of Sciences colloquium, "Molecular Kinesis in Ceiiuiar Function ancl Piasticity," heicl December 7-9, 2000, at the Arnoicl ancl Mabei Beckman Center in Irvine, CA. Abbreviations: eiF, eukaryotic initiation factor; 5' NTR, 5' nontransiatecl region; iRES, internal ribosomai entry site; EMCV, encephaiomyocarclitis virus; FMDV, foot-ancl-mouth clisease virus; TMEV, Theiler's murine encephalitis virus; BVDV, bovine viral cliarrhea virus; CSFV, ciassicai swine fever virus; HCV, hepatitis C virus; ITAF, IRKS transactivating factors; PTB, pyrimiclinetract-bincling protein; RRE, rabbit reticulocyte iysate; GMP-PNP, guanosine 5'-[~S,~-imiclo]
|
From page 34... ...
elFs2, 3 A elFs 4A, 4B elF4F B C A Complex I elFs1, 1A em 4A, 4B elF4F 48S complex elF1 elF1 A ~ Dissociation ~ _ Scanning ~ ~ ~~—~ ~ , ~ i .
|
From page 35... ...
Pestova et a/. G1 G2 G3 G4 G5 G Protein consensus Gx~GKT MAT DxxGH NRxD SAIL IF2 GhvdhGRT tkvaegeaggiT DtpGH NRiD SAT; eIF5B Ghv~tG~T thvgdgeaggiT DtpGH NkiD SAh 638 -645 654 - 665 702-6 756-9 824-6 _ 628 850 1220 552 774 1144 D
|
From page 36... ...
a GTPase that is specifically activated by large and small ribosomal subunits together (17~. This similarity between the homologous factors eIF5B and IF2 suggests that ribosomal activation of their GTPase activity may occur by a common mechanism.
|
From page 37... ...
it?
|
From page 38... ...
jet Illb Ills 11 1 ~ ~ my, ~ ~ IIIe elF3 binding site Primary binding site: essential for 40S subunit attachment IV ~ 00 -- -._ Secondary binding site: needed to place AUG codon in ribosomal mRNA -a LAUGH ~ owning cleft ~ ; 40S subunit binding site Fig.
|
From page 39... ...
attachment to the IRKS to form a stable binary complex, even though they constitute a major site of interaction between these IRESs and the 40S subunit. Similarly, deletion of domain II or mutations in domain IIIa also impaired binding of the initiation codon region to the ribosomal mRNA-binding cleft but did not prevent binary complex formation.
|
From page 40... ...
25. Lamphear, B
|
This material may be derived from roughly machine-read images, and so is provided only to facilitate research.
More
information on Chapter Skim is available.