The National Plant Genome Initiative Objectives for 2003-2008 (2002) / Chapter Skim
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4 Functional Exploitation of Genome Sequences
4 Functional Exploitation of Genome Sequences
Pages 31-36
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From page 31... ...
The Arabidopsis 2010 Functional Genomics Program seeks to associate every known gene in Arabidopsis with a protein or non-protein product so that it can be known where in the cell the product is produced, what biochemical pathway it is part of, and what possible function it has in the life of the organism (NSF 1999~. Just as the finished Arabidopsis genome is greatly facilitating annotation of the rice genome, so will further use of Arabidopsis greatly simplify the functional-genomics challenges presented by the other reference species and in fact by all of plant biology.
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From page 32... ...
We endorse expenditure of funds for technology development and infrastructure that address critical questions specific to plant genomics. For example, the lack of high-throughput, robust transformation systems in many plant species and the lack of gene-replacement techniques are impediments to rapid advancement.
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From page 33... ...
However, other functional-genomics tools, such as protein chips and high-throughput proteomics require substantial cDNA or genome sequence before they can be appropriately designed and deployed in a cost-effective manner. What should be avoided are costly forays into functional genomics technologies and projects that yield partial or ambiguous results, due to incomplete sequence information, and that wiD need to be repeated when the fuD genome sequence becomes available.
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From page 34... ...
These will include comprehensive sets of sequence-indexed mutants, accessible via database search and immediately available as seed stock; robust polymerase chain reaction or chip-based mapping tools; and robust conditional expression systems for sensitized and saturating genetic screens for rare alleles. · High-thro?
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From page 35... ...
Many of the above aims are part of a global approach to the biochemical activities and function of each gene product. Pharmacologic approaches, such as identification of small-molecule inhibitors or activators of gene function, have not been a traditional strength of plant biology.
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From page 36... ...
High-throughput mutant and allele detection systems are also vital, as are reliable systems to detect single-base mismatches. They require appropriate mapping populations, particularly as related to exploitation of natural variation in crop and noncrop species in which the focus is on the identification of valuable alleles, not only the elucidation of gene functions at particular loci.
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