Medically Assisted Conception An Agenda for Research (1989) / Chapter Skim
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Experimental Approaches to the Study of Early Developmental Failure in Human Reproduction
Experimental Approaches to the Study of Early Developmental Failure in Human Reproduction
Pages 234-249
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From page 234... ...
quit occlusion is sty by the relatively law rate at ~id, an ongoing pro is achieved after in Vito fert;1 ization and Irn~ltiple echo transfer at exert IVF preys (between 1# are 25%: Liu et al, 1988; Gibbons, 1988)
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From page 235... ...
Early developmental failure is clearly evident ~' ring in vitro culture, where approximately 10-15% of the normally fertilized human eggs actually develop to the blastocyst stage (Fisher et al, 1985: Lluden berg and Hytell, 1988 )
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From page 236... ...
appears to be an arerestimate that may have resulted fr - ~` ache curatively law nurser of go; Omit, fig patient population included in the study are, for the hypc~aploid ==i~, perhaps freon artifactual 1~= during preparation of ~ oodles for cue ~antitatic~n (Pladh~ et al, 1988; ran Blends ax Henry, 1988~. me reports of Bongos et al (1988)
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From page 237... ...
Anc~h-' approach to the question of the beck grounl frequency of aneuploi~y ~ to determine the chrcncsomal status of meictica~ly mature oocytes obtained from the ovary and prepared for karyotyping immediately preceding the anticipated time of ovulation. W~ameby et al.
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From page 238... ...
lhis method entails the t~ of font, Oral INA stains. Not only were aneuploid Abates detente by this prone, but also of; that exhibited major disorders ~ or =m struck ark ass aviation with the mete spindle were Red.
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From page 239... ...
This particular hypothesis is Katie at present because information concerning the biochemical and spatial nature of the m-11 surface mulches involved in the fertilization process is lacking for the human oocyte. Clearly, an understanding of the temporal and spatial aspects of the first molecular interactions between human garret Is a Rudy prerequisite to det~rmini~ whether, for sax LOCI, subtle biochemical versions are responsible for velc~nta1 failure.
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From page 240... ...
Gibed an armnaly in the stnlc~ organization of the ~rt~x that was Fiats winch the failure of fertilization in gently nor~l-a~aring, ioti~=lly mature Han odes. In this situation, nearly the entire call surface was devoid of ~crovilli, are, in the majority of the s~lasmaler~nal Guam, cortical granules, files arx]
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From page 241... ...
. the rearganizatic~n end polarization of ~ cortical Cytoplasm arm plasma membrane ~ also viewed as a P~*
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From page 242... ...
Electron microscopic studies indicate that the changes in prone clear membrane geometry that typically precede dissolution do not never in eggs arrested with juxtaposed pranuclei pVan Blerkom et al, 1987; Van EDerbom, 1988)
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From page 243... ...
At this rather gross level of analysis, the aphidicol~n and trioKsalen findings suggest that inhibition of pronuclear DNA `~lication in the mouse produces a develcpmenta1 arrest that is phenotypir=1ly similar to the situation that is observed in newly fertilized human eggs.
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From page 244... ...
these particular topics represent only a few of the chr~al and cellular Notions that are end In He preiTrtplantation human embryo (for a c ~ Prehensive Hi~ion, fee Van Elerkom, 1988;1989b)
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From page 245... ...
. Biologic and morphoslogic development of donated human ova recovered by non-surgic~l1 uterine ravage.
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From page 246... ...
. Abnormal chrc~osore behavior in human oocytes which remained unfertile ized Curing human in vitro fertilization.
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From page 247... ...
devel~enbal Is of abberant cellular organization in meiori~1ly mature human of; after exogenous Ovarian hyperstilrnulation.
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From page 248... ...
(fray Van Blerkam, 1989a)
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From page 249... ...
Fi _ 1 and 2: Failed (Fig.
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