Microbial Ecology in States of Health and Disease Workshop Summary (2014) / Chapter Skim
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A16 Topographic diversity of fungal and bacterial communities in human skin--Keisha Findley, Julia Oh, Joy Yang, Sean Conlan, Clayton Deming, Jennifer A. Meyer, Deborah Schoenfeld, Effie Nomicos, Morgan Park, NIH Intramural Sequencing Center Comparative Sequencing Program, Heidi H. Kong, and Julia A. Segre
A16 Topographic diversity of fungal and bacterial communities in human skin--Keisha Findley, Julia Oh, Joy Yang, Sean Conlan, Clayton Deming, Jennifer A. Meyer, Deborah Schoenfeld, Effie Nomicos, Morgan Park, NIH Intramural Sequencing Center Comparative Sequencing Program, Heidi H. Kong, and Julia A. Segre
Pages 401-411
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Eleven core-body and arm sites were dominated by fungi of the genus Malassezia, with only species-level classifications revealing fungal-community composition differences between sites. By contrast, three foot sites -- plantar heel, toenail and toe web -- showed high fungal diversity.
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contribute to challenges to rapidly identify and direct treatment against pathogenic fungi. To compare culture- and DNA-sequence-based identification of human skin associated fungi, we obtained parallel samples from four skin sites of adult healthy volunteers (Supplementary Fig.
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The genus Malassezia predominated at all 11 core-body and arm sites: antecubital fossa, back, external auditory canal, glabella, hypothenar palm, inguinal crease, manubrium, nare, occiput, retroauricular crease and volar forearm (Figure A16-1)
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was taxonomically classified at the genus level, with further resolution of Malassezia species. For all body sites, the left side of the body was used, except for the right toenail of healthy volunteer 7.
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Sites that showed Malassezia predominance at initial sampling displayed the same genus- and species-level predominance with strong community structure stability (Supplementary Figs. 5 and 6, and Supplementary Table 8)
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The values for the core body sites retroauricular crease and glabella are identical and are therefore represented by a single data point on the graph and a shared colour in the key. the skin microbiome is complex and suggest that different characteristics shape skin bacterial and fungal communities.
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at plantar heel, toe web or toenail affected fungal community structure. For uninvolved sites, interpersonal variation in community structure was highly consistent across all
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Plantar heels, toe webs and toenails are common sites of recurrent human fungal disease, which can be recalcitrant to treatment. FIGURE A16-4 Clinical involvement alters shared fungal community structure.
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were added to mechanically disrupt fungal cell walls using Tissuelyser (Qiagen) for 2 min at 30 Hz and then using the PureLink Genomic DNA Kit (Invitrogen)
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The members of the NIH Intramural Sequencing Center Comparative Sequencing program carried out sequencing.
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et al. A tool kit for quantifying eukaryotic rRNA gene sequences from human microbiome samples.
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