An Assessment of the National Institute of Standards and Technology Material Measurement Laboratory Fiscal Year 2014 (2015) / Chapter Skim
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3 Biomolecular Measurement Division
3 Biomolecular Measurement Division
Pages 15-23
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From page 15... ...
The Mass Spectrometry Data Center develops mass spectral libraries for small molecules, metabolites, peptides, glycans, and proteins; the Applied Genetics Group develops standards and technology for DNA testing to aid human identification efforts and to support biometric, law enforcement, and clinical applications using genetic information; the Bioanalytical Science Group develops protein-based standard reference materials such as C-reactive protein (CRP) and bovine serum albumin (BSA)
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From page 16... ...
A major component of this division's work also addresses reference data and spectral libraries for smaller compounds (including biologically relevant low-molecular-weight metabolites and peptides) through the Mass Spectrometry Data Center.
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From page 17... ...
TABLE 3.1 Biomolecular Measurement Division: Summary of Activities and Achievements Group Capability Impact Mass spectrometry data Mass spectrometry of chemicals, NIST/EPA/NIH mass spectral library proteins, peptides, glycans, broadly used in industry metabolites, and lipids Bioprocess measurements Protein particles, interface, and Microfabricated devices, protein characteristics; sensors stability data Biomolecular structure and Macromolecular structure/function IBBR, biosensors, protein therapeutics function Bioanalytical science Small molecule/biomolecules, BSA (protein) standard reference Standard reference material materials, protein metrology development, LC/MS Applied genetics DNA typing, sequencing, Forensic and clinical genetics, DNA multiplex PCR, qPCR, DNA fingerprinting, DNA identification mixture interpretation instruments NOTE: LC/MS, liquid chromatography/mass spectrometry.
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From page 18... ...
Unlike chemical or peptide reference data or samples, protein data need to reflect differences in protein glycosylation patterns, protein cross-linking, refolding patterns, and post translation modification -- that is, changes that occur after the cell expresses or releases the newly formed protein. The technical programs, and particularly the intragroup dynamics, appear to be good.
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From page 19... ...
2366 Cytomegalovirus (CMV) for DNA 2372 Human DNA quantitation standard 2389A Amino acids in 0.1 mol/L HCI 2391C PCR-based DNA profiling 2392 Mitochondrial DNA sequencing 2392-I Mitochondrial DNA sequencing 2393 CAG repeat length mutation in Huntington's disease 2394 Heteroplasmic mitochondrial DNA mutation detection standard 2396 Oxidative DNA damage mass spec.
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From page 20... ...
The recent focus of the BMD on biologics, particularly through the IBBR, is appropriate but also complex, in that the molecules to be studied, and for which reference data and/or standards are needed, are also very complex and manufacturer-specific. However, if the BMD is to successfully address this challenge, close cooperation between the Bioanalytical Science, Bioprocess Measurements, Applied Genetics, and Biomolecular Structure and Function groups will be needed to address standards at each step of the research, development, and, ultimately, manufacturing processes: (1)
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From page 21... ...
DISSEMINATION OF OUTPUTS In addition to the mass spectrometry database and the widely used albumin standard, the outputs of the BMD include DNA standards, biomarkers, and forensic training. 1 In all these cases, the information that is bundled with the standard reference materials has made a major impact on the industry and the ability to manufacture products (even if by different processes)
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From page 22... ...
A BMD strategy integrated with an overall MML strategic plan is needed to clearly map the pathway from current BMD strengths to the development of excellence in applying standard metrics to biomolecules. Recommendation: The Biomolecular Measurement Division should define a strategy that will specify which biomolecules will be selected to be standards, what information is required about those biomolecules, and how they relate to other molecules that are likely to become important in the emerging industry.
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From page 23... ...
These actions are needed if the excellent morale, can-do attitude, and commitment to excellence in measurement results are to be maintained within the BMD. Recommendation: The Biomolecular Measurement Division management should communicate the technical strategic plan to its entire staff, make supervisory processes transparent to staff, and remove, to the extent possible, unnecessary roadblocks to purchasing and collaboration.
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