Skip to main content

(NAS Colloquium) Genetic Engineering of Viruses and Viral Vectors (1996) / Chapter Skim
Currently Skimming:

The function of herpes simplex virus genes: A primer for genetic engineering of novel vectors
Pages 11307-11312

The Chapter Skim interface presents what we've algorithmically identified as the most significant single chunk of text within every page in the chapter.
Select key terms on the right to highlight them within pages of the chapter.


From page 11307...
... genetically engineered replication competent viruses with restricted host range. Whereas the former two types of vectors require stable, continuous cell lines expressing viral genes for their replication, the replication competent viruses will replicate on approved primary human cell strains.
Read the entire page →
From page 11308...
... involved in viral nucleic acid metabolism, reported to localize in nucleoli and in virally induced nuclear dense bodies and to bind to a sequence along with other unidentified proteins. Complex may be involved in cleavage/packaging of viral DNA.
Read the entire page →
From page 11309...
... Small subunit of ribonucleotide reductase. Causes nonspecific degradation of mRNA after infection, shuts off host protein synthesis, enables sequential synthesis of viral proteins.
Read the entire page →
From page 11310...
... 1~. The hypothesis that ICP4 is directly involved in transcrip tion is based on reports that it binds TATA box-binding protein and transcription factor JIB, and on the evidence that after the onset of DNA synthesis, it is a component of ~y-transcriptonsnuclear stuctures containing newly synthesized viral DNA, RNA polymerase II, ICP22, and a cellular protein known as L22 or EAP and that is normally present in nucleoli and Proc.
Read the entire page →
From page 11311...
... As in the case of defective viruses, even replicating viruses will have to carry and express factors that induce immune response or activate pro-drugs selectively in cancer cells. The use of recombinant viruses for prophylaxis against viral infection requires mutants that are genetically stable, incapable of replicating in CNS, incapable of spreading in immuno compromised individuals, unable to reactivate, not transmissible from immunized individual to contacts, but immunogenic and protective against disease caused by subsequent infection.
Read the entire page →
From page 11312...
... regulation of expression of the gene carried by the viral vector, and (iii) regulatory issues related to qualification of continuous cell lines, which express complementing viral genes for replication of defective vectors.
Read the entire page →

Key Terms

  • viral dna
  • dna synthesis
  • inverted repeats
  • viral replication
  • cancer cells

  • cell lines
  • thymidine kinase
  • protein kinase
  • viral genome
  • infected cell

  • herpes simplex
  • recombinant viruses
  • simplex virus
  • latent state
  • defective viruses

  • selective destruction
  • acid metabolism
  • infected cells
  • viral genomes
  • central nervous

  • proteins required
  • cellular genes
  • human genes
  • regulatory protein
  • viral proteins

  • continuous cell
  • genetic engineering
  • nucleic acid
  • glycoprotein required
  • lines expressing


    • This material may be derived from roughly machine-read images, and so is provided only to facilitate research.
    More information on Chapter Skim is available.