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(NAS Colloquium) Genetic Engineering of Viruses and Viral Vectors (1996) / Chapter Skim
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Immunization with DNA vaccines encoding glycoprotein D or glycoprotein B, alone or in combination, induces protective immunity in animal models of herpes simplex virus-2 disease
Pages 11414-11420

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From page 11414...
... In vivo expression is accomplished without the need for live infection or the construction of viral vectors, and the expressed protein or epitopes thereof have the potential to enter the major histocompatibility class I pathway and elicit CD8+ cytotoxic Tlymphocyte responses (26, 35~. Immunization with a cloned gene offers the same specificity as a recombinantly expressed protein subunit vaccine without the complex manufacturing and formulation procedures sometimes required to ensure the immunogenicity of recombinantly expressed proteins.
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From page 11415...
... Having established that low doses of DNA were effective in the mouse model, we then tested the prophylactic effect of a low-dosage combination of these DNAs in the guinea pig vaginal-infection model. Genital disease in guinea pigs closely resembles that in humans (3S, 39)
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From page 11416...
... The results indicated that injection of each DNA construction resulted in gene expression in vivo and the induction of substantial antibody responses, even at low doses. At the lowest dose tested, 0.8 ,ug of gD-2 DNA, eight of nine immunized mice developed detectable antibody responses.
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From page 11417...
... Therefore, the guinea pig vaginal-infection model was used to assay the effects of immunization with a combination of low doses of gD-2 and l~gB-2 DNA. Seven guinea pigs were immunized with a DNA mixture containing 3 ,ug of gD-2 DNA and 10 ,~g of l~gB-2 DNA at weeks O and 6; 14 control guinea pigs were sham-immunized with saline.
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From page 11418...
... This challenge study indicated that immunization with low doses of DNAs that encode HSV-2 full-length gD and a truncated form of gB protected guinea pigs from HSV-2-induced primary genital disease. DISCUSSION Our data show that immunization with DNA encoding fulllength HSV-2 gD or a truncated form of HSV-2 gB induced .
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From page 11419...
... Following challenge, control animals developed severe disease characterized by high lesion scores and the systemic involvement; in contrast, the DNA-immunized guinea pigs were nearly free of disease. Mean lesion scores for the DNA-immunized group were statistically indistinguishable from background.
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From page 11420...
... (1996) in DNA Vaccines, eds.
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Key Terms

  • dna immunization
  • protective immunity
  • genital disease
  • guinea pig
  • lesion scores

  • low doses
  • primary genital
  • cell lysates
  • endpoint titers
  • truncated form

  • herpes simplex
  • immune responses
  • single immunization
  • recombinantly expressed
  • pigs immunized

  • dna vaccine
  • genital herpes
  • dna vaccines
  • antibody responses
  • control animals

  • protein subunit
  • vero cells
  • mouse model
  • serum antibodies
  • vivo expression

  • dna encoding
  • neutralizing serum
  • animals developed
  • mean lesion
  • immune sera


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