Emerging Technologies for Nutrition Research Potential for Assessing Military Performance Capability (1997) / Chapter Skim
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Pages 363-374
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Emerging Technologies for Nutrition Research, 1997 Pp.
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influences of natural abundance and thermodynamic properties do not determine the cellular content and function of a specific metal (da Silva and Williams, 1991)
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ZINC FINGERS Zinc ions serve an important structural function by tetrahedrally coordinating to cysteine or histidine residues of certain proteins to stabilize the structure of a small functional domain. The most prominent role of proteins with zinc-finger motifs is sequence-specific binding to DNA during transcription, and this is one of the most common eukaryotic DNA-binding motifs (Klug and Schwabe, 1995)
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LIM-only proteins, there is mounting evidence that the LIM motif is involved in protein-protein interactions (Schmeichel and Beckerle, 1994)
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might eventually be coupled to gene therapy to provide some degree of control for therapeutic gene expression, as discussed below. MRNA DIFFERENTIAL DISPLAY As international genome mapping projects progress, it has become an increasing priority in biology to identify the genes contained in these vast sequences in order to characterize the function of each gene product.
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The cDNA for each differentially expressed mRNA is recovered from the electrophoresis gel and cloned into a plasmid to maintain a stable copy of cDNA for further analysis. The cDNA is first used as a probe for a Northern blot analysis of the original RNA samples to confirm the differential expression in the actual RNA and to quantify the levels of expression.
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cellular damage caused by ionizing radiation and various chemical toxins (Willson, 1989)
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Kendall, S.D., and M.J. Christensen 1995 Modified differential display identifies genes in rat liver differentially regulated by selenium [abstract]
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ROBERT COUSINS: It was developed in late 1992. There was a very significant modification in 1993.
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ORVILLE LEVANDER: There is quite a bit of work going on with selenium right now in differential display as well. What I was wondering about, Bob, is, on your differential display analysis, that was rat intestine and you had a lot of sequences up there, but is anything happening with metallothionein under those conditions?
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JOHN FERNSTROM: I am ignorant in this area. Is zinc something for which there is a regulated storage pool, such that one can think about the responsiveness from dietary changes as needing to be short responses or long responses?
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