Amphibians Guidelines for the Breeding, Care and Management of Laboratory Animals (1974) / Chapter Skim
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VIII Records and Information Control
VIII Records and Information Control
Pages 108-114
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From page 108... ...
Thus, 35,XXX identifies the clutch or shipment; the hundred's digit may be used to identify groups and the ten's and unit's digits to identify individuals. Serial numbers that do not include letters are recommended to facilitate adaptation to computer techniques, should these be found useful (see Section B below)
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From page 109... ...
describe "chemical branding" as a procedure for marking Xenopus; these are permanent for at least a year. Using a cotton swab dipped in a solution of 0.5 percent amido Schwartz in 7 percent acetic acid, label figures are formed on the back of an animal after mucous secretions are removed by repeated wiping with paper tissues.
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From page 110... ...
However, it is inadequate for urodeles and Xenopus in which the regeneration process replaces the removed digits, unless the regeneration is inhibited by treatment with berilium nitrate (Heatwole, 1961~. Ranidae may be toe clipped shortly after metamorphosis using light anesthesia and cuticle scissors.
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From page 111... ...
This problem can be greatly alleviated by the adoption of computer-based techniques. Recent improvements in available computer programs and time-sharing techniques, even over long-distance telephonic connections, permit even those inexperienced in computer use to adopt these procedures readily.
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From page 112... ...
I PREGULAR BODY PATTERN SPOTS BETWEEN PLICAE POSTERIOR TO EYE ANTERIOR TO VENT 15 OTH ER (NO EXAMPLE) FIGURE 25 Fiana pipiens head and body pattern code characteristics.
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From page 113... ...
~ ~ Ski ~ ~ ~ I ~ NO SPOTS ON ARMS NONE ON | ON LEGS LEGS I L:l i. he F If Or SPOTS ON ARMS NONE ON | ON LEGS LEGS ~ FIGURE 26 Background and other patterns (Nace et a/., 1973; reproduced with permission from the American Zoologist)
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From page 114... ...
AS LONG AS AVERAGE WIDTH i., Irregular One Spot ill: I-, lo; in, Hi\\ Irregular my' Reg ul tar ~ ~ l irte FIGURE 27 Conventions used to characterize spots (Nace et a/., 1973; reproduced with permission from the American Zoologist)
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