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Review of the Research Strategy for Biomass-Derived Transportation Fuels (1999) / Chapter Skim
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Appendix E: Major Components of a Poplar Genomics Initiative
Pages 47-48

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From page 47...
... Experiments for mapping and isolating genes for valuable traits via fine-mapping, 47 intensive studies of gene expression via microarray panels, synteny comparisons to model organisms, and high-throughput transformation. -A -- -1- -- -- STRUCTURAL GENOMICS The following components could be included in the area of structural genomics: · dense microsatellite-based genetic marker maps · dense expressed sequence tag sequence banks · physical mapping via bacterial artificial chromosomes · expression chips (microarrays)
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From page 48...
... 48 wood chemistry and structure disease resistance shoot phonology and stress tolerance maturation, flowering onset and sterility, and rootability FUNCTIONAL GENOMICS Research on functional genomics could include studies in the following areas: BIOMASS-DERIVED TRANSPORTATION FUELS high-precision quantitative trait loci analysis and synteny-based candidate gene selection transformation tests of candidate genes selected from expressed sequence tag banks complementation, suppression, and overexpression tests of identified genes via transformation large population of activation-tagged transgenic trees to directly identify genes for diverse traits additional bacterial artificial chromosome libraries for trait-specific experiments
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Key Terms

  • structural genomics
  • sequence tag
  • bacterial artificial
  • expressed sequence
  • trait expression

  • genomics project
  • artificial chromosomes
  • contrasting trait
  • studying trait
  • ribonucleic acids

  • synteny comparisons
  • physical mapping
  • synteny relationships
  • field experiments
  • recurring annual

  • feedstock species
  • map synteny
  • artificial chromosome
  • experimental materials
  • significant program

  • materials based
  • key traits
  • outlined using
  • tag sequence
  • using poplars

  • annual cost
  • options outlined
  • dense expressed
  • single feedstock
  • model organisms


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