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Preventing the Forward Contamination of Europa (2000) / Chapter Skim
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5 Microbial Detection and Identification
Pages 20-21

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From page 20...
... has been used to confirm that microbial populations detected at the DNA level are metabolically active.7 Also, viable microbes have an intact membrane that contains phospholipids. Cellular enzymes hydrolyze the phosphate group from phospholipids within minutes to hours of cell death.8 Therefore, determination of the total amount of phospholipid ester-linked fatty acids (PLEA)
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From page 21...
... 7 M.M. Moeseneder et al., " Optimization of Terminal-Restriction Fragment Length Polymorphism Analysis for Complex Marine Bacterioplankton Communities and Comparison with Denaturing Gradient Gel Electrophoresis," Applied Environmental Microbiology 65: 3518, 1999.
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Key Terms

  • chemical species
  • planetary protection
  • asm press
  • cell death
  • genes encoding

  • microbial biomass
  • standard growth
  • capability using
  • reduced chemical
  • membrane lipid

  • acceptable bioburden
  • virtually unchanged
  • molecular criteria
  • carbon monoxide
  • improved detection

  • inorganic compounds
  • molecular methods
  • growth medium
  • detection capability
  • marine bacterioplankton

  • heat shock
  • ribonucleic acids
  • denaturing gradient
  • energy requirements
  • encoding enzymes

  • survived heat
  • sedimentary microbial
  • using explicit
  • particular classes
  • cellular biopolymers


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