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Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

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Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

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Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

INTRODUCTION

Safe and secure execution of experiments will minimize health and safety risks to laboratory personnel and those in the surrounding area. When institutional laboratory standards are not sufficient to mitigate the risks, laboratory personnel may use the Moderate Risk Standard Operating Procedure (SOP) or the High Risk SOP for the specific process.

MODERATE RISK STANDARD OPERATING PROCEDURE

A Moderate Risk SOP is suggested for processes, experiments, or manipulations that pose moderate risks and that call for protective steps beyond those dictated by accepted laboratory standards. The Moderate Risk SOP form is a condensed version of the High Risk SOP.

Moderate Risk Standard Operating Procedure Form

Implementation of the Moderate Risk SOP is suggested for processes, experiments, or manipulations that pose moderate risks and that call for protective steps beyond those dictated by accepted laboratory standards. They are intended to limit the potential for injury, equipment damage, or environmental impact.

1. TITLE AND DESCRIPTION OF EXPERIMENT OR PROCESS:

2. PREPARER(S):

3. LOCATION:

4. AUTHORIZED PERSONNEL (WITH CONTACT INFORMATION)

a. PRINCIPAL INVESTIGATOR (PI)/SUPERVISOR:

b. STUDENT(S)/TECHNICIAN(S)/OPERATOR(S):

c. OTHERS TO BE NOTIFIED (e.g., other workers in the same laboratory, or other members of the research group):

5. POTENTIAL HAZARDS:

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

6. DESCRIBE ALL SPECIAL REQUIREMENTS FOR SPECIFIC ITEMS THAT REQUIRE A GREATER LEVEL OF SAFETY

Planned chemicals involved

Personal protective equipment

Engineering and environmental controls

Operational ranges and conditions

Special handling and storage requirements

Spill and accident procedure as needed

Waste handling and disposal

7. IDENTIFY ANY TRAINING NEEDS

Specific training required

Users requiring training

Date completed

8. VERIFICATION AND REVIEW

By signing below, you acknowledge that you have read, understand, and approve the SOP.

PI name (please print)
Pl signature
Safety staff name (please print)
Safety staff signature
Current date
Date of SOP expiration (e.g., 1 year)
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

9. LIST OF REFERENCES (include Safety Data Sheets, Globally Harmonized System, any outside personnel consulted in preparation of document, peer reviewers, etc.)

images

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

Moderate Risk Standard Operating Procedure Review and Modifications Log

Were modifications made to current SOP? Yes images No images
If no, then list date of approval
Last reviewed by (please print)
If yes, then list and describe modifications


Were modifications made to current SOP? Yes images No images
If no, then list date of approval
Last reviewed by (please print)
If yes, then list and describe modifications


Were modifications made to current SOP? Yes images No images
If no, then list date of approval
Last reviewed by (please print)
If yes, then list and describe modifications


The worker will first record basic information on the Moderate Risk SOP form, including the title of the experiment or operation, the name of the person(s) preparing the SOP, where the experiment or procedure will take place, and the names of authorized personnel.

Identifiable potential hazards (Step 5) are those that laboratory personnel are aware of before carrying out the experiment or procedure. Hazards may be posed by any chemical of concern, particularly if handled by inexperienced workers, as well as any reaction products that may be generated either in situ or as intended products or byproducts.

The most crucial item in the Moderate Risk SOP is Step 6: “Describe all special requirements for specific items that require a greater level of safety.” Laboratory personnel are encouraged to review all available resources (see Appendix B), such as the literature, existing SOPs, and Safety Data Sheets (SDSs), and consult with other members at the institution to complete Step 6 with as much detail as possible.

  1. Personal protective equipment (PPE) includes gloves, goggles, safety glasses, face shield, laboratory coat, special shoes (i.e., toe protection), respirator, and other items required for the experiment or procedure.
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
  1. Engineering and environmental controls refer to the space and environment in which the experiment or procedure will take place, such as a fume hood or an inert atmosphere.
  2. Special handling and storage requirements are described in detail to prevent spills or accidents. Handling and storage procedures include, for example, use of specific glassware (e.g., open container, Schlenk flask) or techniques that can minimize spills and accidents.
  3. Spill and accident procedures should be listed in the event of an accident.
  4. Procedures for handling and disposing of waste will assist the laboratory worker in the proper and safe disposal of the chemical reagents, solvents, and byproducts and any disposable equipment, such as paper towels and pipettes.

After the form has been completed, senior staff will review the SOP for completeness and accuracy. A modifications log appears at the end of the form for laboratory personnel who carried out the same process but modified or updated some of the information on the form. Each facility is responsible for establishing what necessitates a modification to the SOP. Supervisors, professors, and chemical hygiene officers are all acceptable positions for review and approval of modifications and updates.

HIGH RISK STANDARD OPERATING PROCEDURE

If after completing the Risk Assessment Flowchart, the laboratory worker has determined that the chemical(s) or conditions pose a high risk for hazard, then he or she should develop a High Risk SOP. The High Risk SOP can be applied to processes, experiments, or manipulations that are not addressed by the institution’s acceptable laboratory standards and are considered to present high risks for both inexperienced and experienced laboratory workers.

As with the Moderate Risk SOP, the High Risk SOP form begins with basic information (i.e., experiment title, name of the person preparing the SOP, experiment location, and names of authorized personnel). The second item calls for a description of the techniques and manipulations to be employed and any specific training that is required to complete the experiment or procedure safely, such as for proper use of a pressurized gas or for manipulations on a Schlenk line.

High Risk Standard Operating Procedure Form

Implementation of the High Risk SOP is suggested for processes, experiments, or manipulations that pose high risks and that call for protective steps beyond those dictated by accepted laboratory standards. They are intended to limit the potential for injury, equipment damage, or environmental impact.

1. TITLE AND DESCRIPTION OF EXPERIMENT OR PROCESS:

2. PREPARER(S):

3. LOCATION:

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

4. AUTHORIZED PERSONNEL (WITH CONTACT INFORMATION)

a. PRINCIPAL INVESTIGATOR (PI)/SUPERVISOR:

b. STUDENT(S)/TECHNICIAN(S)/OPERATOR(S):

c. OTHERS TO BE NOTIFIED (e.g., other workers in the same laboratory, or other members of the research group):

5. IDENTIFY ANY TRAINING NEEDS

Experimental techniques

Specific training required

Users requiring training

Date completed

6. DETAILED PROCESS DESCRIPTION

List ranges for variables

Temperature:
Pressure:
Viscosity:
Flammability:
Other:
List operational ranges and conditions
Materials to be used Chemicals:

Equipment:

DETAILED PROCEDURE:

Column A: Process Steps provide step-by-step normal operating procedures, including the transfer of chemicals between or across laboratories, production of gases, workup of products, and preparation of wastes.

Column B: Safety Notes describe how the risk can be mitigated (i.e., minimize clutter, control flammables, secure gas cylinders, ensure good condition of pump belts).

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Column A: Process Steps Column B: Safety Notes
1.0


e.g., specific personal protective equipment requirements
1.1


1.2


2.0


2.1


2.2


3.0


3.1


3.2


Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
4.0


4.1


4.2


7. ENGINEERING CONTROLS (check all that apply and provide a detailed description)

Engineering Controls Check box if applicable Description
Ex. Glove box images Use inert air (N2 or Ar)

Fume hood or glove box

images

Special ventilation images
HEPA-filtered vacuum lines images
Non-reactive containers images
Pressure relief devices images
Temperature control images
Bench paper, pads, plastic-backed paper images
Special signage images
Safe sharp devices images
Other safety devices used: images
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

8. PERSONAL PROTECTIVE EQUIPMENT (check all that apply)

Personal Protective Equipment          Check box if applicable Description
Gloves images
Lab coats images
Suits images
Aprons images
Long pants images
Close-toed shoes images
Long sleeves images
Safety glasses images
Goggles images
Face shields images
Respirators (include cartridge type and cartridge change-out schedule) images
Hearing protection (include level of protection needed) images
Special equipment (i.e., blast shields, special enclosures) images
Other PPE images
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

9. WORK PRACTICE CONTROLS

Controls Description
Designated areas


Procedures for requesting emergency assistance


Emergency phone numbers


Locations of fire alarms, fire extinguishers, fire blankets, eye washes, showers, etc.


Emergency responders


Workers on shifts


Training on all experimental techniques and experiments


Restricting access; locks


Housekeeping


Lockout/tagouta procedure plan


After-hour procedures


Preventive maintenance


a Lockout/tagout refers to specific procedures to safeguard researchers from an unexpected startup of machinery and equipment, or a release of hazardous energy during service or maintenance activities.

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

10. MONITORING

Monitoring Description
Personnel exposure monitoring e.g., wearable sensors for toxics, radiation badges


Leak checking


Gas and spill release monitoring


Temperature and pressure


Alarms


11. SPILL AND ACCIDENT PROCEDURES

Description and Location
Secondary containment


Spill kits


Emergency shutdown procedures


Process shutdown


Persons to inform


12. WASTE DISPOSAL PROCEDURES (include segregation of acids, bases, halogenated compounds, PPE, collection and transport procedures, proper documentation, regulations, etc.)

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

13. STORAGE (check all that apply)

Ventilated enclosures images

Refrigeration images

Gas cabinet images

Compliance with regulations images

Expiration date:

Inventory:

Other:

14. TRANSPORTATION PROCEDURES:

(e.g., secondary container for transporting between and across laboratories; secondary containment for experimental apparatus)


15. PEER REVIEW

Name (please print)
Signature
Date
Notes


Name (please print)
Signature
Date
Notes


Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

16. VERIFICATION AND REVIEW

By signing below, you acknowledge that you have read, understand, and approve the SOP.

PI name (please print)
PI signature
Safety staff name (please print)
Safety staff signature
Current Date
Date of SOP expiration (e.g., 1 year)

17. LIST OF REFERENCES (include Safety Data Sheets, Globally Harmonized System, any outside personnel consulted in preparation of document, peer reviewers, etc.)

images

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

High Risk Standard Operating Procedure Review and Modifications Log

Were modifications made to current SOP? Yes images No images
If no, then list date of approval
Last reviewed by (please print)
If yes, then list and describe modifications


Were modifications made to current SOP? Yes images No images
If no, then list date of approval
Last reviewed by (please print)
If yes, then list and describe modifications


Were modifications made to current SOP? Yes images No images
If no, then list date of approval
Last reviewed by (please print)
If yes, then list and describe modifications


Laboratory personnel are strongly encouraged to review other resources as thoroughly as possible to inform completion of Step 6 of the High Risk SOP. Physical properties of the chemicals that can change during the experiment (e.g., temperature, pressure, viscosity) should be listed clearly, as well as the ranges of acceptable values. The chemicals and equipment that will be used during the experiment, as well as the scale of the experiment (i.e., size of equipment and quantities of chemicals), should also be described.

Normal operating procedures (Step 6 of the High Risk SOP) must be detailed step by step in column A, “Process Steps.” For many processes, the steps must be followed in precise order. The process steps may include the following:

  • transfer of a chemical between laboratories
  • valve closing and opening
  • product workup
  • preparation of wastes
  • predicted hazard of expected product(s) of a chemical procedure
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

Column B, “Safety Notes,” describes how the risk can be mitigated, for example by

  • controlling flammables
  • securing gas cylinders
  • ensuring that vacuum pump belts are in good condition
  • minimizing clutter

Steps 7 and 8 list the engineering controls and PPE to use during the experiment or procedure. A descriptions of an item that requires more detailed information should be added next to the checkbox. For example, the glove type can be specified (e.g., nitrile, vinyl, butyl) or, if multiple gloves are to be used, the order of wear can be specified.

Steps 9-13 relate to the laboratory setting. This section will include information to assist the worker(s) conducting the experiment and people in the surrounding vicinity in the event of an accident. Although not recommended, if a worker is conducting an experiment alone, then security staff and other on-site laboratory personnel must check on that worker periodically. In case of an emergency, the worker must notify emergency staff. Finally, in Step 14, even though transportation procedures may have been described in Step 6, include them here, with details on items such as secondary containers for transport of chemicals between laboratories as needed.

Senior staff will review the SOP for completeness and accuracy. Each institution will establish what constitutes the need for modifications, which will be recorded in the modifications log. Supervisors, professors, and chemical hygiene officers are all acceptable positions for review and approval of modifications and updates.

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×

This page intentionally left blank.

Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 19
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 20
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 21
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 22
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 23
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 24
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 25
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 26
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 27
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 28
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 29
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 30
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 31
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 32
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 33
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 34
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 35
Suggested Citation:"4 STANDARD OPERATING PROCEDURE FORMS." National Academies of Sciences, Engineering, and Medicine. 2016. Chemical Laboratory Safety and Security: A Guide to Developing Standard Operating Procedures. Washington, DC: The National Academies Press. doi: 10.17226/21918.
×
Page 36
Next: 5 APPLICATION OF THE *GUIDE TO DEVELOPING STANDARD OPERATING PROCEDURES* »
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The U.S. Department of State charged the Academies with the task of producing a protocol for development of standard operating procedures (SOPs) that would serve as a complement to the Chemical Laboratory Safety and Security: A Guide to Prudent Chemical Management and be included with the other materials in the 2010 toolkit. To accomplish this task, a committee with experience and knowledge in good chemical safety and security practices in academic and industrial laboratories with awareness of international standards and regulations was formed. The hope is that this toolkit expansion product will enhance the use of the previous reference book and the accompanying toolkit, especially in developing countries where safety resources are scarce and experience of operators and end-users may be limited.

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