Chemical Communication in a Post-Genomic World (2003) / Chapter Skim
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6 Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans
6 Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans
Pages 19-24
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From page 19... ...
To assess its function in viva, we expressed TRPV4 in Casnorhabditis elegant sensory neurons and examined its ability to generate behavioral responses to sensory stimuli. C elegant ASH neurons function as polymodal sensory neurons that generate a characteristic escape behavior in response to mechanical, osmotic, or olfactory stimuli.
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elegans behavior; this result is consistent with the relatively slow activation of vertebrate osmosensory channels. To confirm that osmotic avoidance ar~d nose touch avoidance were generated by TRPV4 expression in ASH, the ASH neurons were ablated in osm-9 ASH::trpv4 animals by using a laser microbeam.
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From page 21... ...
TRPV4-expressing animals avoided hyperosmotic stimuli, but in heterologous expression systems, TRPV4-expressing cells are activated only in response to hypoosmolar stimuli (22, 234. This difference suggests that the ASH sensory neuron might contain accessory molecules that allow TRPV4 to sense hyperosmotic stimuli.
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Bar graphs depict the combined results for three independent transgenic lines of each mutant. The first three bars represent the same results depicted in Fig.
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From page 23... ...
Our results provide evidence that mammalian TRPV4 can function as a central component of the sensor for osmotic and mechanical stimuli in ASH sensory neurons in C elegans.
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From page 24... ...
12. Bargmann, C
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