Chemical Communication in a Post-Genomic World (2003) / Chapter Skim
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4 Pheromone-mediated gene expression in the honey bee brain
4 Pheromone-mediated gene expression in the honey bee brain
Pages 7-13
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From page 7... ...
We also report here on an ortholog of the Drosophila transcription factor kruppel homolog 1 that was strongly regulated by QMP, especially in the mushroom bodies of the bee brain. These results demonstrate chronic gene regulation by a primer pheromone and illustrate the potential of genomics to trace the actions of a pheromone from perception to action, and thereby provide insights into how pheromones regulate social life.
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From page 8... ...
Notably, the expression of most significantly regulated genes changed by ~10%. For example, of 2,607 eDNAs found to be significantly regulated during the time course, only 1,158 showed differences of >10%, and only 111 showed differences of >20%.
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From page 9... ...
match Similarity score Fold difference QMP+/QMP Possible function Nurse/forager Q9H2Y7 (human) CG14168 cit Traf l CG7474 poe frizzled 2 kr-h 1 2E-08 1E-13 5E-23 1 E-24 4E-85 9E-34 5E-96 1E-100 1.11 1.12 1.16 1.16 1.17 0.86 0.70 0.59 WD-repeat protein PDZ domain Carboxylesterase Tumor necrosis factor Forager receptor-associated factor Tubulinyl-tyrosine ligase Calmodulin binding, synpatogenesis G protein-coupled receptor Zn-finger transcription factor Nurse Nurse Forager Forager Genes found to be chronically regulated by QMP with high sequence similarity to known Drosophila or human genes are listed below.
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From page 10... ...
Because transcription factors regulate the expression of other genes, they may serve as markers for regulation of pheromone-mediated "transcriptional programs" that control downstream behavioral effects. A total of 39 of the 129 cDNAs annotated as transcription factors (16)
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From page 11... ...
· — Discussion This study has demonstrated that QMP causes changes in expression levels of many genes in the brain of adult honey bees, and that these changes correlate with some of the downstream behavioral effects of the pheromone. QMP effects on gene expression were detected both in a controlled laboratory environment and in bee colonies in the field, which represent a more natural environment.
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From page 12... ...
To aid in the future identification of specific transcriptional programs, we catalogued the transcription factors whose expression levels were significantly regulated by QMP. Only one transcription factor was found to be chronically regulated; most showed significant changes in expression on only 1 day.
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