Designing Nanostructures at the Interface between Biomedical and Physical Systems Conference Focus Group Summaries (2005) / Chapter Skim
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Sequence a Single Molecule of Protein
Sequence a Single Molecule of Protein
Pages 39-44
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From page 39... ...
The most basic level, determining the primary structure, involves sequencing the polypeptide chain. Even state-of-the-art commercial sequencing techniques require picomolar samples, equivalent to micrograms of protein or ~1013 molecules.
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From page 40... ...
7:715-718. FOCUS GROUP SUMMARY Summary written by: Maureen McDonough, Graduate Student, Science Writing Program, Massachusetts Institute of Technology Focus group members: · David Auston, President, Kavli Foundation · Mark Hersam, Assistant Professor, Department of Materials Science and Engineering, Northwestern University · Abraham Lee, Professor, Department of Biomedical Engineering, University of California, Irvine · Luke Lee, Professor, Department of Bioengineering, University of California, Berkeley
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From page 41... ...
However, many proteins exist naturally in extremely small quantities: an individual cell may only have one or two copies of specific hormones and transcription factors. The ability to sequence these proteins would help in determining their structure; and, by combining sequence and structure information, large amounts of a specific protein could be produced and used in therapies.
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From page 42... ...
Someone else continued to stress the importance of using information available from the sequence of the human genome, by checking the determined amino acid sequence against known DNA sequences. The differences in opinion regarding the ideal solution led to a tendency for individuals to interject with statements or questions that would pull the discussion toward the idea in which they were most interested.
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From page 43... ...
The sample chamber needs to be scaled down to a single molecule. The SERS detection chamber needs an optimized surface substrate, and Raman signature spectra for each of the 20 amino acids need to be determined.
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From page 44... ...
Another solution involved the riboswitch model. If DNA could be released instead of a quantum dot, then each piece of DNA representing an amino acid could be ligated to the previous piece, creating a sequence of DNA that corresponded to the amino acid sequence.
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