Frontiers of Engineering Reports on Leading-Edge Engineering from the 2009 Symposium (2010) / Chapter Skim
Currently Skimming:
Digital Holographic Microscopy for3D Imaging of Complex Fluids and Biological Systems--Vinothan N. Manoharan
Digital Holographic Microscopy for3D Imaging of Complex Fluids and Biological Systems--Vinothan N. Manoharan
Pages 5-12
The Chapter Skim interface presents what we've algorithmically identified as the most significant single chunk of text within every page in the chapter.
Select key terms on the right to highlight them within pages of the chapter.
From page 5... ...
Confocal microscopy and related tech niques provide quantitative characterizations of both the structure and dynamics of systems of many interacting microscopic components. In materials science and physics, these real-space imaging techniques have revealed in enormous detail the mechanisms of phase transitions and nonequilibrium phenomena such as glass formation -- mechanisms that cannot in general be resolved using ensembleaveraging techniques such as scattering (van Blaaderen and Wiltzius, 1995; Weeks et al., 2000; Yethiraj and van Blaaderen, 2003)
|
From page 6... ...
FIGURE 1 Top left: Hologram formation from a point source showing a plane reference wave interfering with a spherical scattered wave to produce (center, calculated) a Gabor zone plate pattern.
|
From page 7... ...
If the object consists of many point particles distributed through space, then the interference pattern is simply the coherent superposition of Gabor zone plates. As Gabor first noted, when the scattering from the particles is weak, one can shine a plane wave back through the hologram (the photograph of the interference pattern)
|
From page 8... ...
HOLOGRAPHIC MICROSCOPY FOR STUDYING SELF-ASSEMBLY IN COMPLEX FLUIDS We use DHM primarily for studying the self-assembly of complex fluids, and in particular colloidal and nanoparticle suspensions; the particles in these systems have diameters of 10 to 1,000 nm. Interactions among these particles can drive them to self-assemble into ordered structures at equilibrium, such as col loidal crystals, which can serve as the basis for advanced functional materials like photonic crystals (Vlasov et al., 2001)
|
From page 9... ...
Thus processing a typical time series of holograms takes several days on a personal workstation, and for each 1 gigabyte holographic "movie," we can generate nearly a terabyte of volumetric reconstructions. In the meantime, although work remains to be done before DHM can replace confocal microscopy as a general tool, it can be used to probe specific systems and important scientific questions.
|
From page 10... ...
coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation.
|
From page 11... ...
2005. Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantita tive visualization of living cells with subwavelength axial accuracy.
|
Key Terms
This material may be derived from roughly machine-read images, and so is provided only to facilitate research.
More
information on Chapter Skim is available.