Agricultural Development and Environmental Research American and Czechoslovak Perspectives: Proceedings of a Bilateral Workshop (1987) / Chapter Skim
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'GENE ENGINEERING AND TRANSFORMATION OF THE PLANT GENOME BY AGROBACTERIUM VECTORS'
'GENE ENGINEERING AND TRANSFORMATION OF THE PLANT GENOME BY AGROBACTERIUM VECTORS'
Pages 165-169
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From page 165... ...
Among the several methods of introducing foreign genes, the most developed is the use of vectors derived from the Ti plasmid of Agrobacterium tumefaciens. This naturally occurring soil bacterium causes tumor growth in dicotyledenous plant tissues due to the integration of a particular segment of the Ti plasmid of A
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From page 166... ...
There is no doubt that this technology will change the concept of dicotyledonous cultivated plants for future plant breeding. The Department of Plant Breeding Theory of the CSAV Institute of Experimental Botany is studying the effects of integration of TDNA into the genome of different dicotyledonous plant species from three points of view: • Introduction of the whole, unmodified T-DNA of different Ti plasmids of A
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From page 167... ...
In still the next generation, opine synthesis was shown only by a small proportion of plants, but most opine negative plants showed the presence of T-DNA by the Southern blotting test. DNA methylation was therefore suspected as the probable cause of the disappearance of opine synthesizing activity.
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From page 168... ...
At present, we are interested in vectors which do not integrate oncogens into the plant genome, but only selectable genes for kanamycin resistance together with other cloned genes. We are interested in integrating virus cDNA sequences into the plant genome, which can introduce virus disease resistance to the genomes of cultivated plants by several mechanisms: • By transcription of anti-RNA, which is capable of forming double strands with mRNA tn vivo.
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From page 169... ...
1984. Gametic transmission of mannopine and agropine synthesis in Arabidopiu thaliana hairy root tumor renerants.
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