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From page 119... ... extracellular enzymes. In the latter part of his career Doucloroff and his associates extensively cIarifiec3 taxonomic and phylogenetic relationships in the genus Pseudomonas and certain other aerobic bacteria. Read the entire page →
From page 120... ... 120 BIOGRAPHICAL MEMOIRS sequently was appointee! naval attache to the Russian embassy in Japan. Read the entire page →
From page 121... ... saccharophila catalyze a reaction between sucrose and inorganic phosphate to form glucose I-phosphate and fructose. Since the reaction proved to be readily reversible, it was used to synthesize sucrose, a sugar not previously synthesized by either chemical or enzymatic Read the entire page →
From page 122... ... Attempts by Weimberg and Doudoroff to demonstrate directly the formation of a glycosyl-enzyme complex in the sucrose phosphorylase reaction were unsuccessful because of insufficient purification of the enzyme by the methods then available and because of an intrinsic hydrolytic activity of the enzyme. After better purification techniques were Read the entire page →
From page 123... ... A second type of phosphorolytic enzyme, maltose phosphorylase, was found in Neisseria meningatidis by Doudoroff and Fitting (1952,21. Extracts of this organism had been shown previously to catalyze a reaction between the disaccharide maltose and orthophosphate to form glucose and a phosphate ester with properties similar to those of glu Read the entire page →
From page 124... ... The mechanism of action of maltose phosphorylase was shown to stiffer from that of sucrose phosphorylase. The former, unlike the latter, is unable to catalyze a direct exchange between 13-D-glucose T-phosphate anct orthophosphate or arsenate and is unable to cause an exchange between maltose and glucose in the absence of the phosphate ester. Read the entire page →
From page 125... ... M I C H AE L D O U D O RO F F 125 phosphate. Doudoroff and O'Neal (1945,3) Read the entire page →
From page 126... ... 126 BIOGRAPHICAL MEMOIRS of the keto acid, ketodeoxyphosphogluconate aldolase, was later purified and crystallized by Doudoroff and Shuster (1967~. Several other sugars were shown to be metabolized by P Read the entire page →
From page 127... ... were stimulated to develop a more general explanation of oxidative assimilation by the observation of their colleague, Germaine Cohen-Bazire, that purple bacteria accumulate massive amounts of polyp-hyclroxybutyric acid during growth on certain organic acids. Poly-~-hydroxybutyric acid (PHB) Read the entire page →
From page 128... ... 128 BIOGRAPHICAL MEMOIRS similation from acetate and butyrate in Rhodospirillum rubrum and found with all those substrates that a major fraction (60 to 90 percent) of the assimilated carbon initially accumulatec! Read the entire page →
From page 129... ... MICHAEL DOUDOROFF 129 granules, derived from cells that synthesize the polymer, can be hydrolyzed. Even the granules proved to be very labile. Read the entire page →
From page 130... ... in c olIaboration with Stanie r, Norberto Palleroni , and several graduate students and postdoctoral fellows macle extensive contributions to knowledge of the taxonomy of pseuclomonacis and certain other aerobic bacteria. The first publication resulting from this investigation was a massive survey of 169 phenotypic characters of 267 strains of Pseudomonas (Doudoroff et al., ~ 966, ~ ~ . Read the entire page →
From page 131... ... MICHAEL DOUDOROFF 13 of a much larger number of nutritionally diverse characters than had previously been used for this purpose. Although the initial classification of pseudomonads was based entirely on phenot,Ypic characters, Palleroni, Dou~doroff' and associates later used the newer techniques of DNADNA and ribosomal RNA-DNA hybridization to investigate the genotypic and phylogenetic relations among species. Read the entire page →
From page 132... ... 132 BIOGRAPHICAL MEMOIRS supported separation of these organisms into two genera, Moraxella and Acinetobacter, on the basis of a cytochrome cdependent oxidase reaction, and recognized one species of Acinetobacter and several species of Moraxella on the basis of correlated phenotypic properties. Doudoroff exerted a profound influence on the teaching of bacteriology at Berkeley. Read the entire page →
From page 133... ... at the Pasteur Institute. In 1960-62 he held a Miller Research Professorship at the University of California, Berkeley, and in 1963 he was awarded a National Institutes of Health Special Postdoctoral Fellowship for studies with Professor Georges N Read the entire page →

From page 119...

... extracellular enzymes. In the latter part of his career Doucloroff and his associates extensively cIarifiec3 taxonomic and phylogenetic relationships in the genus Pseudomonas and certain other aerobic bacteria.