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STATEMENT OF THE PROBLEM This chapter develops methods to predict the strand separation and helix destabilization experienced by a specified DNA sequence when superhelically stressed. We will focus specifically on predictions regarding several plasmids (that is, circular DNA molecules)
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From page 185... ...
This yields a total of 2N distinct states of strand separation. This precludes the use of exact methocis, in which all states are enumerated, to analyze molecules of biological interest, as these commonly have lengths exceeding 1,000 base pairs.
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