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Calculating the Secrets of Life Contributions of the Mathematical Sciences to Molecular Biology (1995) / Chapter Skim
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Pages 13-15

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From page 13...
... PCR allows one to (Erectly amplify a specific DNA sequence without resort to cloning. To perform PCR, one uses short DNA molecules called primers (typically about 20 bases long)
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From page 14...
... 14 CALCULATING THE SECRETS OF LIFE Foreign D NA to be insured + '(at | Joining / Recombinant DNA molecule PIasmid vector Introduction into host cells by transformation or viral infection ich romosome | Selection for cells contain ing a recombinant DNA molecule \ / ,|, Cloning ~(05 FIGURE 1.8 By cloning a foreign DNA molecule in a plasmid vector, it is possible to propagate the DNA in a bacterial or other host cell.
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From page 15...
... The method involves successive rounds of copying (using the enzyme DNA polymerase) between two synthetic primers corresponding to nearby DNA sequences.
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Key Terms

  • dna molecules
  • dna molecule
  • dna sequence
  • dna sequences
  • million base

  • successive rounds


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