Calculating the Secrets of Life Contributions of the Mathematical Sciences to Molecular Biology (1995) / Chapter Skim
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Pages 195-198
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From page 195... ...
This demonstrates that these analytical methods provide highly precise predictions of the details of strand separation in superhelical molecules. APPLYING THE METHOD TO STUDY INTERESTING GENES Having developed a method and confirmed its accuracy on test molecules, we can now apply it to study any DNA sequence of interest.
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From page 196... ...
This result suggests that genes in bacterial DNA can be partitioned into two categories, depending on whether or not they are bracketed by superhelically destabilized regulatory regions. In a similar vein, we have analyzed the DNA sequences of two mammalian viruses, the polyoma and papilloma viruses, each of which can cause cancer.
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From page 197... ...
push a wave of positive supercoiTs ahead and leave a wake of negative supercoils behind (Wu et al., 1988~. A region of strand separation constitutes a localized concentration of negative superhelicity, due to the large decrease in twist that occurs.
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From page 198... ...
If one role of DNA superhelicity is to promote strand separation, one would expect to find highly destabilized sites abutting the origin of replication. In fact, the calculations show precisely this, providing strong support for the assumption.
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