Calculating the Secrets of Life Contributions of the Mathematical Sciences to Molecular Biology (1995) / Chapter Skim
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Pages 203-206
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The topological approach to enzymology is an experimental protocol in which the descriptive and analytical powers of topology and geometry are employed in an indirect effort to determine the enzyme mechanism and the structure of active enzyme-DNA complexes in vitro (in a test tube) (Wasserman and Cozzarelli, 1986; Sumners, 1 987a)
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From page 204... ...
In the classical Crick-Watson double helix mode} for DNA, the ladder is twisted in a right-hand helical fashion, with an average and nearly constant pitch of approximately 10.5 base pairs per full helical twist. The local helical pitch of duplex DNA is a function of both the local base pair sequence and the cellular environment in which the DNA lives; if a DNA molecule is under stress, or constrained to live on the surface of a protein, or is being acted upon by an enzyme, the helical pitch can change.
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From page 205... ...
These DNA knots and links are observed by gel electrophoresis and electron microscopy of the reaction product DNA molecules. By observing the changes in geometry (supercoiling)
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From page 206... ...
figure eight catenane (Krasnow et al., 1983) , and Figure 8.lc shows a micrograph of the DNA knot 6~*
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